Nrf2 gene promoter polymorphism is associated with ulcerative colitis in a Japanese population

Tomiyasu Arisawa, Tomomitsu Tahara, Tomoyuki Shibata, Mitsuo Nagasaka, Masakatsu Nakamura, Yoshio Kamiya, Hiroshi Fujita, Daisuke Yoshioka, Masaaki Okubo, Mikiju Sakata, Fang Yu Wang, Ichiro Hirata, Hiroshi Nakano

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background/Aims: Transcription factor Nrf2 regulates the expression of detoxifying and antioxidant genes. Three promoter polymorphisms of this gene have been identified. This study attempted to clarify the relationship between Nrf2 gene polymorphism and ulcerative colitis in a Japanese population. Methodology: The study was performed in 89 patients with ulcerative colitis (mean age: 40.2 years, M:F=47:42) and 141 healthy volunteers (mean age: 38.7 years, M:F=75:66). The PCR-SSCP method was employed to detect Nrf2 polymorphisms using DNA extracted from peripheral blood cells. Results: Comparison of genotype frequency, -686·-684 A·G/A·G genotype was significantly lower in the UC group than those in the HC group (OR=0.45, 95%CI:0.22-0.93, p=0.029) and G·G carrier was higher in the UC group, especially in female subjects. Furthermore, -686·-684 G·G carrier was more closely associated with chronic continuous phenotype (OR=2.57, 95%CI:1.01-6.60, p=0.043). On the other hand, no association between -650 genotype and ulcerative colitis was found. Conclusions: The -686·-684 genotype of Nrf2 gene may be associated with the development of ulcerative colitis.

Original languageEnglish
Pages (from-to)394-397
Number of pages4
JournalHepato-gastroenterology
Volume55
Issue number82-83
Publication statusPublished - 01-03-2008

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Ulcerative Colitis
Genotype
Population
Genes
Single-Stranded Conformational Polymorphism
Blood Cells
Healthy Volunteers
Transcription Factors
Antioxidants
Phenotype
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Arisawa, T., Tahara, T., Shibata, T., Nagasaka, M., Nakamura, M., Kamiya, Y., ... Nakano, H. (2008). Nrf2 gene promoter polymorphism is associated with ulcerative colitis in a Japanese population. Hepato-gastroenterology, 55(82-83), 394-397.
Arisawa, Tomiyasu ; Tahara, Tomomitsu ; Shibata, Tomoyuki ; Nagasaka, Mitsuo ; Nakamura, Masakatsu ; Kamiya, Yoshio ; Fujita, Hiroshi ; Yoshioka, Daisuke ; Okubo, Masaaki ; Sakata, Mikiju ; Wang, Fang Yu ; Hirata, Ichiro ; Nakano, Hiroshi. / Nrf2 gene promoter polymorphism is associated with ulcerative colitis in a Japanese population. In: Hepato-gastroenterology. 2008 ; Vol. 55, No. 82-83. pp. 394-397.
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title = "Nrf2 gene promoter polymorphism is associated with ulcerative colitis in a Japanese population",
abstract = "Background/Aims: Transcription factor Nrf2 regulates the expression of detoxifying and antioxidant genes. Three promoter polymorphisms of this gene have been identified. This study attempted to clarify the relationship between Nrf2 gene polymorphism and ulcerative colitis in a Japanese population. Methodology: The study was performed in 89 patients with ulcerative colitis (mean age: 40.2 years, M:F=47:42) and 141 healthy volunteers (mean age: 38.7 years, M:F=75:66). The PCR-SSCP method was employed to detect Nrf2 polymorphisms using DNA extracted from peripheral blood cells. Results: Comparison of genotype frequency, -686·-684 A·G/A·G genotype was significantly lower in the UC group than those in the HC group (OR=0.45, 95{\%}CI:0.22-0.93, p=0.029) and G·G carrier was higher in the UC group, especially in female subjects. Furthermore, -686·-684 G·G carrier was more closely associated with chronic continuous phenotype (OR=2.57, 95{\%}CI:1.01-6.60, p=0.043). On the other hand, no association between -650 genotype and ulcerative colitis was found. Conclusions: The -686·-684 genotype of Nrf2 gene may be associated with the development of ulcerative colitis.",
author = "Tomiyasu Arisawa and Tomomitsu Tahara and Tomoyuki Shibata and Mitsuo Nagasaka and Masakatsu Nakamura and Yoshio Kamiya and Hiroshi Fujita and Daisuke Yoshioka and Masaaki Okubo and Mikiju Sakata and Wang, {Fang Yu} and Ichiro Hirata and Hiroshi Nakano",
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Arisawa, T, Tahara, T, Shibata, T, Nagasaka, M, Nakamura, M, Kamiya, Y, Fujita, H, Yoshioka, D, Okubo, M, Sakata, M, Wang, FY, Hirata, I & Nakano, H 2008, 'Nrf2 gene promoter polymorphism is associated with ulcerative colitis in a Japanese population', Hepato-gastroenterology, vol. 55, no. 82-83, pp. 394-397.

Nrf2 gene promoter polymorphism is associated with ulcerative colitis in a Japanese population. / Arisawa, Tomiyasu; Tahara, Tomomitsu; Shibata, Tomoyuki; Nagasaka, Mitsuo; Nakamura, Masakatsu; Kamiya, Yoshio; Fujita, Hiroshi; Yoshioka, Daisuke; Okubo, Masaaki; Sakata, Mikiju; Wang, Fang Yu; Hirata, Ichiro; Nakano, Hiroshi.

In: Hepato-gastroenterology, Vol. 55, No. 82-83, 01.03.2008, p. 394-397.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nrf2 gene promoter polymorphism is associated with ulcerative colitis in a Japanese population

AU - Arisawa, Tomiyasu

AU - Tahara, Tomomitsu

AU - Shibata, Tomoyuki

AU - Nagasaka, Mitsuo

AU - Nakamura, Masakatsu

AU - Kamiya, Yoshio

AU - Fujita, Hiroshi

AU - Yoshioka, Daisuke

AU - Okubo, Masaaki

AU - Sakata, Mikiju

AU - Wang, Fang Yu

AU - Hirata, Ichiro

AU - Nakano, Hiroshi

PY - 2008/3/1

Y1 - 2008/3/1

N2 - Background/Aims: Transcription factor Nrf2 regulates the expression of detoxifying and antioxidant genes. Three promoter polymorphisms of this gene have been identified. This study attempted to clarify the relationship between Nrf2 gene polymorphism and ulcerative colitis in a Japanese population. Methodology: The study was performed in 89 patients with ulcerative colitis (mean age: 40.2 years, M:F=47:42) and 141 healthy volunteers (mean age: 38.7 years, M:F=75:66). The PCR-SSCP method was employed to detect Nrf2 polymorphisms using DNA extracted from peripheral blood cells. Results: Comparison of genotype frequency, -686·-684 A·G/A·G genotype was significantly lower in the UC group than those in the HC group (OR=0.45, 95%CI:0.22-0.93, p=0.029) and G·G carrier was higher in the UC group, especially in female subjects. Furthermore, -686·-684 G·G carrier was more closely associated with chronic continuous phenotype (OR=2.57, 95%CI:1.01-6.60, p=0.043). On the other hand, no association between -650 genotype and ulcerative colitis was found. Conclusions: The -686·-684 genotype of Nrf2 gene may be associated with the development of ulcerative colitis.

AB - Background/Aims: Transcription factor Nrf2 regulates the expression of detoxifying and antioxidant genes. Three promoter polymorphisms of this gene have been identified. This study attempted to clarify the relationship between Nrf2 gene polymorphism and ulcerative colitis in a Japanese population. Methodology: The study was performed in 89 patients with ulcerative colitis (mean age: 40.2 years, M:F=47:42) and 141 healthy volunteers (mean age: 38.7 years, M:F=75:66). The PCR-SSCP method was employed to detect Nrf2 polymorphisms using DNA extracted from peripheral blood cells. Results: Comparison of genotype frequency, -686·-684 A·G/A·G genotype was significantly lower in the UC group than those in the HC group (OR=0.45, 95%CI:0.22-0.93, p=0.029) and G·G carrier was higher in the UC group, especially in female subjects. Furthermore, -686·-684 G·G carrier was more closely associated with chronic continuous phenotype (OR=2.57, 95%CI:1.01-6.60, p=0.043). On the other hand, no association between -650 genotype and ulcerative colitis was found. Conclusions: The -686·-684 genotype of Nrf2 gene may be associated with the development of ulcerative colitis.

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Arisawa T, Tahara T, Shibata T, Nagasaka M, Nakamura M, Kamiya Y et al. Nrf2 gene promoter polymorphism is associated with ulcerative colitis in a Japanese population. Hepato-gastroenterology. 2008 Mar 1;55(82-83):394-397.