TY - JOUR
T1 - Observation of 100 nm periodic fibrils in mouse corneal stroma by cryoultramicrotomy
AU - Nakamura, Makoto
AU - Kobayashi, Miya
AU - Hanaichi, Takamasa
AU - Hirano, Koji
AU - Kobayashi, Kunihiko
AU - Hoshino, Takeshi
AU - Awaya, Shinobu
N1 - Funding Information:
Acknowledgments. This study was supported in part by a grant from the Ministry of Education, Science and Culture, Japan. No. 03-77-121 7.
PY - 1993/6
Y1 - 1993/6
N2 - We observed mouse comeal stroma after treatment with 20 mM adenosine 5'-triphosphate (ATP), thin-sectioned by cryoultramicrotomy, with a transmission electron microscope. A wide array of 100 nm periodic fibrils (100 nm PFs) was observed among D-periodic collagen fibrils. By indirect immuno-cryoultramicrotomy with anti-type VI collagen antibody, immunogold particles were located on lOOnm PFs, indicating that lOOnm PFs were composed of type VI collagen.Cryoultramicrotomy is.useful for studying the ultrastructure of the extracellular matrix of the corneal stroma.
AB - We observed mouse comeal stroma after treatment with 20 mM adenosine 5'-triphosphate (ATP), thin-sectioned by cryoultramicrotomy, with a transmission electron microscope. A wide array of 100 nm periodic fibrils (100 nm PFs) was observed among D-periodic collagen fibrils. By indirect immuno-cryoultramicrotomy with anti-type VI collagen antibody, immunogold particles were located on lOOnm PFs, indicating that lOOnm PFs were composed of type VI collagen.Cryoultramicrotomy is.useful for studying the ultrastructure of the extracellular matrix of the corneal stroma.
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U2 - 10.1093/oxfordjournals.jmicro.a051029
DO - 10.1093/oxfordjournals.jmicro.a051029
M3 - Article
C2 - 8376924
AN - SCOPUS:0027616927
SN - 2050-5698
VL - 42
SP - 185
EP - 188
JO - Microscopy
JF - Microscopy
IS - 3
ER -