Observation of 100 nm periodic fibrils in mouse corneal stroma by cryoultramicrotomy

Makoto Nakamura, Miya Kobayashi, Takamasa Hanaichi, Koji Hirano, Kunihiko Kobayashi, Takeshi Hoshino, Shinobu Awaya

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

We observed mouse comeal stroma after treatment with 20 mM adenosine 5'-triphosphate (ATP), thin-sectioned by cryoultramicrotomy, with a transmission electron microscope. A wide array of 100 nm periodic fibrils (100 nm PFs) was observed among D-periodic collagen fibrils. By indirect immuno-cryoultramicrotomy with anti-type VI collagen antibody, immunogold particles were located on lOOnm PFs, indicating that lOOnm PFs were composed of type VI collagen.Cryoultramicrotomy is.useful for studying the ultrastructure of the extracellular matrix of the corneal stroma.

Original languageEnglish
Pages (from-to)185-188
Number of pages4
JournalMicroscopy
Volume42
Issue number3
DOIs
Publication statusPublished - 06-1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Instrumentation
  • Radiology Nuclear Medicine and imaging

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