Observation of 100 nm periodic fibrils in mouse corneal stroma by cryoultramicrotomy

Makoto Nakamura; Miya Kobayashi; Takamasa Hanaichi; Koji Hirano; Kunihiko Kobayashi; Takeshi Hoshino; Shinobu Awaya

doi:10.1093/oxfordjournals.jmicro.a051029

Observation of 100 nm periodic fibrils in mouse corneal stroma by cryoultramicrotomy

Makoto Nakamura
, Miya Kobayashi
, Takamasa Hanaichi
, Koji Hirano
, Kunihiko Kobayashi
, Takeshi Hoshino
, Shinobu Awaya

ophthalmology

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We observed mouse comeal stroma after treatment with 20 mM adenosine 5'-triphosphate (ATP), thin-sectioned by cryoultramicrotomy, with a transmission electron microscope. A wide array of 100 nm periodic fibrils (100 nm PFs) was observed among D-periodic collagen fibrils. By indirect immuno-cryoultramicrotomy with anti-type VI collagen antibody, immunogold particles were located on lOOnm PFs, indicating that lOOnm PFs were composed of type VI collagen.Cryoultramicrotomy is.useful for studying the ultrastructure of the extracellular matrix of the corneal stroma.

Original language	English
Pages (from-to)	185-188
Number of pages	4
Journal	Microscopy
Volume	42
Issue number	3
DOIs	https://doi.org/10.1093/oxfordjournals.jmicro.a051029
Publication status	Published - 06-1993

All Science Journal Classification (ASJC) codes

General Medicine

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10.1093/oxfordjournals.jmicro.a051029

Cite this

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title = "Observation of 100 nm periodic fibrils in mouse corneal stroma by cryoultramicrotomy",

abstract = "We observed mouse comeal stroma after treatment with 20 mM adenosine 5'-triphosphate (ATP), thin-sectioned by cryoultramicrotomy, with a transmission electron microscope. A wide array of 100 nm periodic fibrils (100 nm PFs) was observed among D-periodic collagen fibrils. By indirect immuno-cryoultramicrotomy with anti-type VI collagen antibody, immunogold particles were located on lOOnm PFs, indicating that lOOnm PFs were composed of type VI collagen.Cryoultramicrotomy is.useful for studying the ultrastructure of the extracellular matrix of the corneal stroma.",

author = "Makoto Nakamura and Miya Kobayashi and Takamasa Hanaichi and Koji Hirano and Kunihiko Kobayashi and Takeshi Hoshino and Shinobu Awaya",

note = "Funding Information: Acknowledgments. This study was supported in part by a grant from the Ministry of Education, Science and Culture, Japan. No. 03-77-121 7.",

year = "1993",

month = jun,

doi = "10.1093/oxfordjournals.jmicro.a051029",

language = "English",

volume = "42",

pages = "185--188",

journal = "Microscopy",

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TY - JOUR

T1 - Observation of 100 nm periodic fibrils in mouse corneal stroma by cryoultramicrotomy

AU - Nakamura, Makoto

AU - Kobayashi, Miya

AU - Hanaichi, Takamasa

AU - Hirano, Koji

AU - Kobayashi, Kunihiko

AU - Hoshino, Takeshi

AU - Awaya, Shinobu

N1 - Funding Information: Acknowledgments. This study was supported in part by a grant from the Ministry of Education, Science and Culture, Japan. No. 03-77-121 7.

PY - 1993/6

Y1 - 1993/6

N2 - We observed mouse comeal stroma after treatment with 20 mM adenosine 5'-triphosphate (ATP), thin-sectioned by cryoultramicrotomy, with a transmission electron microscope. A wide array of 100 nm periodic fibrils (100 nm PFs) was observed among D-periodic collagen fibrils. By indirect immuno-cryoultramicrotomy with anti-type VI collagen antibody, immunogold particles were located on lOOnm PFs, indicating that lOOnm PFs were composed of type VI collagen.Cryoultramicrotomy is.useful for studying the ultrastructure of the extracellular matrix of the corneal stroma.

AB - We observed mouse comeal stroma after treatment with 20 mM adenosine 5'-triphosphate (ATP), thin-sectioned by cryoultramicrotomy, with a transmission electron microscope. A wide array of 100 nm periodic fibrils (100 nm PFs) was observed among D-periodic collagen fibrils. By indirect immuno-cryoultramicrotomy with anti-type VI collagen antibody, immunogold particles were located on lOOnm PFs, indicating that lOOnm PFs were composed of type VI collagen.Cryoultramicrotomy is.useful for studying the ultrastructure of the extracellular matrix of the corneal stroma.

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DO - 10.1093/oxfordjournals.jmicro.a051029

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SN - 2050-5698

VL - 42

SP - 185

EP - 188

JO - Microscopy

JF - Microscopy

IS - 3

ER -

Observation of 100 nm periodic fibrils in mouse corneal stroma by cryoultramicrotomy

Abstract

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