Okadaic acid reverses the inhibitory effect of protein kinase C on alkaline phosphatase activity in osteoblast-like cells

Yasuko Watanabe, Osamu Kozawa, Atsushi Suzuki, Jun Kotoyori, Yoshiaki Ito, Yutaka Oiso

Research output: Contribution to journalArticle

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Abstract

We previously reported that fetal calf serum-induced alkaline phosphatase activity is suppressed due to the activation of protein kinase C in osteoblast-like MC3T3-E1 cells (Miwa et al. (1991) Bone Miner. 14, 15-25; Kotoyori et al. (1994) Horm. Metab. Res. 26, 116-118). In the present study, we examined the effect of okadaic acid, a potent and specific inhibitor of protein phosphatase type 1 and 2A, on fetal calf serum-induced alkaline phosphatase activity in MC3T3-E1 cells. The pretreatment with okadaic acid enhanced the fetal calf serum-induced alkaline phosphatase activity in a dose-dependent manner in the range between 0.1 and 5 nM. 1-Norokadaone, a less potent analogue of okadaic acid, had little effect on the fetal calf serum-induced alkaline phosphatase activity. Okadaic acid partially reversed the suppression of fetal calf serum-induced alkaline phosphatase activity by 12-O-tetradecanoylphorbol-13-acetate, a protein kinase C activator. The effect of okadaic acid was dose-dependent in the range between 0.1 and 5 nM. The patterns of the dosedependency of both okadaic acid effects on fetal calf serum-induced alkaline phosphatase activity and on the suppression by 12-O-tetradecanoylphorbol-13-acetate were similar. These results strongly suggest that protein phosphatase type 1 and/or 2A act as a regulator of alkaline phosphatase activity at a point downstream from protein kinase C in osteoblast-like cells.

Original languageEnglish
Pages (from-to)115-118
Number of pages4
JournalMolecular and Cellular Endocrinology
Volume103
Issue number1-2
DOIs
Publication statusPublished - 01-01-1994

Fingerprint

Okadaic Acid
Osteoblasts
Protein Kinase C
Alkaline Phosphatase
Serum
Protein Phosphatase 2
Tetradecanoylphorbol Acetate
Acetates
Protein Phosphatase 1
Miners
Bone
Chemical activation
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Watanabe, Yasuko ; Kozawa, Osamu ; Suzuki, Atsushi ; Kotoyori, Jun ; Ito, Yoshiaki ; Oiso, Yutaka. / Okadaic acid reverses the inhibitory effect of protein kinase C on alkaline phosphatase activity in osteoblast-like cells. In: Molecular and Cellular Endocrinology. 1994 ; Vol. 103, No. 1-2. pp. 115-118.
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Okadaic acid reverses the inhibitory effect of protein kinase C on alkaline phosphatase activity in osteoblast-like cells. / Watanabe, Yasuko; Kozawa, Osamu; Suzuki, Atsushi; Kotoyori, Jun; Ito, Yoshiaki; Oiso, Yutaka.

In: Molecular and Cellular Endocrinology, Vol. 103, No. 1-2, 01.01.1994, p. 115-118.

Research output: Contribution to journalArticle

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