Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection

Yoshinori Ito; Yukiko Shibata-Watanabe; Yoko Ushijima; Jun Ichi Kawada; Yukihiro Nishiyama; Seiji Kojima; Hiroshi Kimura

doi:10.1086/527330

Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection

Yoshinori Ito, Yukiko Shibata-Watanabe, Yoko Ushijima, Jun Ichi Kawada, Yukihiro Nishiyama, Seiji Kojima, Hiroshi Kimura

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Chronic active Epstein-Barr virus infection (CAEBV) is characterized by recurrent infectious mononucleosis-like symptoms and has high mortality and morbidity. To clarify the mechanisms of CAEBV, the gene-expression profiles of peripheral blood obtained from patients with CAEBV were investigated. Twenty genes were differentially expressed in 4 patients with CAEBV. This microarray result was verified using a real-time reverse-transcriptase polymerase chain reaction assay in a larger group of patients with CAEBV. Eventually, 3 genes were found to be significantly upregulated: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5. These genes may be associated with the inflammatory reaction or with cell proliferation.

Original language	English
Pages (from-to)	663-666
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	197
Issue number	5
DOIs	https://doi.org/10.1086/527330
Publication status	Published - 01-03-2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Infectious Diseases

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10.1086/527330

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Ito, Y., Shibata-Watanabe, Y., Ushijima, Y., Kawada, J. I., Nishiyama, Y., Kojima, S., & Kimura, H. (2008). Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection. Journal of Infectious Diseases, 197(5), 663-666. https://doi.org/10.1086/527330

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title = "Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection",

abstract = "Chronic active Epstein-Barr virus infection (CAEBV) is characterized by recurrent infectious mononucleosis-like symptoms and has high mortality and morbidity. To clarify the mechanisms of CAEBV, the gene-expression profiles of peripheral blood obtained from patients with CAEBV were investigated. Twenty genes were differentially expressed in 4 patients with CAEBV. This microarray result was verified using a real-time reverse-transcriptase polymerase chain reaction assay in a larger group of patients with CAEBV. Eventually, 3 genes were found to be significantly upregulated: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5. These genes may be associated with the inflammatory reaction or with cell proliferation.",

author = "Yoshinori Ito and Yukiko Shibata-Watanabe and Yoko Ushijima and Kawada, {Jun Ichi} and Yukihiro Nishiyama and Seiji Kojima and Hiroshi Kimura",

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Ito, Y, Shibata-Watanabe, Y, Ushijima, Y, Kawada, JI, Nishiyama, Y, Kojima, S & Kimura, H 2008, 'Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection', Journal of Infectious Diseases, vol. 197, no. 5, pp. 663-666. https://doi.org/10.1086/527330

Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection. / Ito, Yoshinori; Shibata-Watanabe, Yukiko; Ushijima, Yoko et al.
In: Journal of Infectious Diseases, Vol. 197, No. 5, 01.03.2008, p. 663-666.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection

AU - Ito, Yoshinori

AU - Shibata-Watanabe, Yukiko

AU - Ushijima, Yoko

AU - Kawada, Jun Ichi

AU - Nishiyama, Yukihiro

AU - Kojima, Seiji

AU - Kimura, Hiroshi

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AB - Chronic active Epstein-Barr virus infection (CAEBV) is characterized by recurrent infectious mononucleosis-like symptoms and has high mortality and morbidity. To clarify the mechanisms of CAEBV, the gene-expression profiles of peripheral blood obtained from patients with CAEBV were investigated. Twenty genes were differentially expressed in 4 patients with CAEBV. This microarray result was verified using a real-time reverse-transcriptase polymerase chain reaction assay in a larger group of patients with CAEBV. Eventually, 3 genes were found to be significantly upregulated: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5. These genes may be associated with the inflammatory reaction or with cell proliferation.

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Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection

Abstract

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