Oncogenicity of the ret transforming gene in MMTV/ret transgenic mice

Takashi Iwamoto; Masahide Takahashi; Masafumi Ito; Michinari Hamaguchi; Ken Ichi Isobe; Nobuaki Misawa; Jun Pei Asai; Tomoaki Yoshida; Izumi Nakashima

Oncogenicity of the ret transforming gene in MMTV/ret transgenic mice

Takashi Iwamoto, Masahide Takahashi, Masafumi Ito, Michinari Hamaguchi, Ken Ichi Isobe, Nobuaki Misawa, Jun Pei Asai, Tomoaki Yoshida, Izumi Nakashima

Medical Biology

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44 Citations (Scopus)

Abstract

We have successfully produced transgenic mice that carry the ret oncogene driven by a mouse mammary tumor virus promoter/enhancer. Mammary and salivary gland adenocarcinomas were developed in a stochastic fashion in these mice. Moreover, premalignant tumors with hyperplastic and dysplastic lesions of Harderian glands and male reproductive tracts frequently occurred at young ages. High expression of the transgene was closely associated with the development of these tumors although the levels of the transgene expression were variable among individuals. In addition, large amounts of phosphotyrosine-containing proteins were detected in cell lysales from mammary and salivary adenocarcinomas by immunoblotting with the anti-phosphotyrosine antibody.

Original language	English
Pages (from-to)	535-542
Number of pages	8
Journal	Oncogene
Volume	5
Issue number	4
Publication status	Published - 04-1990

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics
Cancer Research

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Cite this

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title = "Oncogenicity of the ret transforming gene in MMTV/ret transgenic mice",

abstract = "We have successfully produced transgenic mice that carry the ret oncogene driven by a mouse mammary tumor virus promoter/enhancer. Mammary and salivary gland adenocarcinomas were developed in a stochastic fashion in these mice. Moreover, premalignant tumors with hyperplastic and dysplastic lesions of Harderian glands and male reproductive tracts frequently occurred at young ages. High expression of the transgene was closely associated with the development of these tumors although the levels of the transgene expression were variable among individuals. In addition, large amounts of phosphotyrosine-containing proteins were detected in cell lysales from mammary and salivary adenocarcinomas by immunoblotting with the anti-phosphotyrosine antibody.",

author = "Takashi Iwamoto and Masahide Takahashi and Masafumi Ito and Michinari Hamaguchi and Isobe, {Ken Ichi} and Nobuaki Misawa and Asai, {Jun Pei} and Tomoaki Yoshida and Izumi Nakashima",

year = "1990",

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language = "English",

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pages = "535--542",

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T1 - Oncogenicity of the ret transforming gene in MMTV/ret transgenic mice

AU - Iwamoto, Takashi

AU - Takahashi, Masahide

AU - Ito, Masafumi

AU - Hamaguchi, Michinari

AU - Isobe, Ken Ichi

AU - Misawa, Nobuaki

AU - Asai, Jun Pei

AU - Yoshida, Tomoaki

AU - Nakashima, Izumi

PY - 1990/4

Y1 - 1990/4

N2 - We have successfully produced transgenic mice that carry the ret oncogene driven by a mouse mammary tumor virus promoter/enhancer. Mammary and salivary gland adenocarcinomas were developed in a stochastic fashion in these mice. Moreover, premalignant tumors with hyperplastic and dysplastic lesions of Harderian glands and male reproductive tracts frequently occurred at young ages. High expression of the transgene was closely associated with the development of these tumors although the levels of the transgene expression were variable among individuals. In addition, large amounts of phosphotyrosine-containing proteins were detected in cell lysales from mammary and salivary adenocarcinomas by immunoblotting with the anti-phosphotyrosine antibody.

AB - We have successfully produced transgenic mice that carry the ret oncogene driven by a mouse mammary tumor virus promoter/enhancer. Mammary and salivary gland adenocarcinomas were developed in a stochastic fashion in these mice. Moreover, premalignant tumors with hyperplastic and dysplastic lesions of Harderian glands and male reproductive tracts frequently occurred at young ages. High expression of the transgene was closely associated with the development of these tumors although the levels of the transgene expression were variable among individuals. In addition, large amounts of phosphotyrosine-containing proteins were detected in cell lysales from mammary and salivary adenocarcinomas by immunoblotting with the anti-phosphotyrosine antibody.

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AN - SCOPUS:0025259593

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Oncogenicity of the ret transforming gene in MMTV/ret transgenic mice

Abstract

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