ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE

Yecan Wang; Kohji Murakami; Toshihiro Kasama; Satoru Shinkawa; Shigenobu Mitsuzawa; Ryo Miyake; Madoka Takai

ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE

Yecan Wang
, Kohji Murakami
, Toshihiro Kasama
, Satoru Shinkawa
, Shigenobu Mitsuzawa
, Ryo Miyake
, Madoka Takai

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Conventional competitive enzyme-linked immunosorbent assay (ELISA) measuring the cortisol level in body fluid consumes considerable time over 1 hour, resulting from sophisticated procedures and precise control of reagent addition. The decrease in the detection time is the significant for the point of care testing and health monitoring. In order to eliminate the time limitations, we developed an automatic one-click microfluidic system on the basis of competitive ELISA and electrospun polystyrene microfiber-based substrates.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1535-1536
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 2021
Externally published	Yes
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 10-10-2021 → 14-10-2021

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10-10-21 → 14-10-21

All Science Journal Classification (ASJC) codes

Bioengineering
Chemical Engineering (miscellaneous)

Cite this

Wang, Y., Murakami, K., Kasama, T., Shinkawa, S., Mitsuzawa, S., Miyake, R., & Takai, M. (2021). ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1535-1536). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Wang, Yecan ; Murakami, Kohji ; Kasama, Toshihiro et al. / ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 1535-1536 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

@inproceedings{2ccabdfc46fb49c08dd433c97e9b78e3,

title = "ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE",

abstract = "Conventional competitive enzyme-linked immunosorbent assay (ELISA) measuring the cortisol level in body fluid consumes considerable time over 1 hour, resulting from sophisticated procedures and precise control of reagent addition. The decrease in the detection time is the significant for the point of care testing and health monitoring. In order to eliminate the time limitations, we developed an automatic one-click microfluidic system on the basis of competitive ELISA and electrospun polystyrene microfiber-based substrates.",

keywords = "Competitive ELISA, Cortisol, Microfibrous substrate, Microfluidic system, Rapid detection",

author = "Yecan Wang and Kohji Murakami and Toshihiro Kasama and Satoru Shinkawa and Shigenobu Mitsuzawa and Ryo Miyake and Madoka Takai",

note = "Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

language = "English",

series = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "1535--1536",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

}

Wang, Y, Murakami, K, Kasama, T, Shinkawa, S, Mitsuzawa, S, Miyake, R & Takai, M 2021, ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 1535-1536, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10-10-21.

ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE. / Wang, Yecan; Murakami, Kohji; Kasama, Toshihiro et al.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 1535-1536 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE

AU - Wang, Yecan

AU - Murakami, Kohji

AU - Kasama, Toshihiro

AU - Shinkawa, Satoru

AU - Mitsuzawa, Shigenobu

AU - Miyake, Ryo

AU - Takai, Madoka

PY - 2021

Y1 - 2021

N2 - Conventional competitive enzyme-linked immunosorbent assay (ELISA) measuring the cortisol level in body fluid consumes considerable time over 1 hour, resulting from sophisticated procedures and precise control of reagent addition. The decrease in the detection time is the significant for the point of care testing and health monitoring. In order to eliminate the time limitations, we developed an automatic one-click microfluidic system on the basis of competitive ELISA and electrospun polystyrene microfiber-based substrates.

AB - Conventional competitive enzyme-linked immunosorbent assay (ELISA) measuring the cortisol level in body fluid consumes considerable time over 1 hour, resulting from sophisticated procedures and precise control of reagent addition. The decrease in the detection time is the significant for the point of care testing and health monitoring. In order to eliminate the time limitations, we developed an automatic one-click microfluidic system on the basis of competitive ELISA and electrospun polystyrene microfiber-based substrates.

KW - Competitive ELISA

KW - Cortisol

KW - Microfibrous substrate

KW - Microfluidic system

KW - Rapid detection

UR - https://www.scopus.com/pages/publications/85136954703

UR - https://www.scopus.com/pages/publications/85136954703#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:85136954703

T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 1535

EP - 1536

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Wang Y, Murakami K, Kasama T, Shinkawa S, Mitsuzawa S, Miyake R et al. ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 1535-1536. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

ONE-CLICK MICROFLUDIC SYSTEM FOR RAPID DETECTION OF CORTISOL BY COMPETITIVE ELISA WITH ELECTROSPUN MICROFIBER SUBSTRATE

Abstract

Publication series

Conference

All Science Journal Classification (ASJC) codes

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