TY - JOUR
T1 - Ontogeny and Multipotency of Neural Crest-Derived Stem Cells in Mouse Bone Marrow, Dorsal Root Ganglia, and Whisker Pad
AU - Nagoshi, Narihito
AU - Shibata, Shinsuke
AU - Kubota, Yoshiaki
AU - Nakamura, Masaya
AU - Nagai, Yasuo
AU - Satoh, Etsuko
AU - Morikawa, Satoru
AU - Okada, Yohei
AU - Mabuchi, Yo
AU - Katoh, Hiroyuki
AU - Okada, Seiji
AU - Fukuda, Keiichi
AU - Suda, Toshio
AU - Matsuzaki, Yumi
AU - Toyama, Yoshiaki
AU - Okano, Hideyuki
PY - 2008/4/10
Y1 - 2008/4/10
N2 - Although recent reports have described multipotent, self-renewing, neural crest-derived stem cells (NCSCs), the NCSCs in various adult rodent tissues have not been well characterized or compared. Here we identified NCSCs in the bone marrow (BM), dorsal root ganglia, and whisker pad and prospectively isolated them from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. Cultured EGFP-positive cells formed neurosphere-like structures that expressed NCSC genes and could differentiate into neurons, glial cells, and myofibroblasts, but the frequency of the cell types was tissue source dependent. Interestingly, we observed NCSCs in the aorta-gonad-mesonephros region, circulating blood, and liver at the embryonic stage, suggesting that NCSCs migrate through the bloodstream to the BM and providing an explanation for how neural cells are generated from the BM. The identification of NCSCs in accessible adult tissue provides a new potential source for autologous cell therapy after nerve injury or disease.
AB - Although recent reports have described multipotent, self-renewing, neural crest-derived stem cells (NCSCs), the NCSCs in various adult rodent tissues have not been well characterized or compared. Here we identified NCSCs in the bone marrow (BM), dorsal root ganglia, and whisker pad and prospectively isolated them from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. Cultured EGFP-positive cells formed neurosphere-like structures that expressed NCSC genes and could differentiate into neurons, glial cells, and myofibroblasts, but the frequency of the cell types was tissue source dependent. Interestingly, we observed NCSCs in the aorta-gonad-mesonephros region, circulating blood, and liver at the embryonic stage, suggesting that NCSCs migrate through the bloodstream to the BM and providing an explanation for how neural cells are generated from the BM. The identification of NCSCs in accessible adult tissue provides a new potential source for autologous cell therapy after nerve injury or disease.
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U2 - 10.1016/j.stem.2008.03.005
DO - 10.1016/j.stem.2008.03.005
M3 - Article
C2 - 18397758
AN - SCOPUS:41449116604
SN - 1934-5909
VL - 2
SP - 392
EP - 403
JO - Cell Stem Cell
JF - Cell Stem Cell
IS - 4
ER -