Optical measurement of glutamate in slice preparations of the mouse retina

M. Ohkuma, M. Kaneda, S. Yoshida, A. Fukuda, E. Miyachi

Research output: Contribution to journalArticle

Abstract

Signaling by glutamatergic synapses plays an important role in visual processing in the retina. In this study, we used an enzyme-linked fluorescence assay system to monitor the dynamics of extracellular glutamate in a slice preparation from the mouse retina. High K stimulation induced an elevation of fluorescence in the inner plexiform layer (IPL) of the retina when glutamate transporters were inhibited by DL-threo-β-benzyloxyaspartic acid (TBOA). The high K-induced fluorescence signals in the IPL were inhibited by the calcium channel blocker Cd2+. Blockade of GABAergic and glycinergic circuits by picrotoxin and strychnine also elevated the fluorescence signals in the IPL. Thus, the enzyme-linked fluorescence assay system might be useful for monitoring the bulk concentration of extracellular glutamate released by synapses in the inner retina.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalNeuroscience Research
Volume137
DOIs
Publication statusPublished - 12-2018

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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