TY - JOUR
T1 - Optimal patch application time in the evaluation of skin irritation
AU - Kanto, Hiromi
AU - Washizaki, Kumiko
AU - Ito, Masatoshi
AU - Matsunaga, Kayoko
AU - Akamatsu, Hirohiko
AU - Kawai, Keiichi
AU - Katoh, Norito
AU - Natsuaki, Masaru
AU - Yoshimura, Isao
AU - Kojima, Hajime
AU - Okamoto, Yuko
AU - Okuda, Minehiro
AU - Kuwahara, Hirofumi
AU - Sugiyama, Mariko
AU - Kinoshita, Shigemi
AU - Mori, Fukuyoshi
PY - 2013/5
Y1 - 2013/5
N2 - We investigated the optimum application for evaluating skin irritation response by using samples of irritants commonly used as additives in cosmetics and other common household products. We studied 47 volunteers (16 men and 31 women). We selected three types of surfactant, one moisturizer, one anti-infective agent and one oil solution. Using Finn chambers on Scanpor tape, we performed the patch test. A total of 0.015 mL of each sample was applied to the Finn chamber. For liquids, circular filter paper was soaked in 0.015 mL of the sample. Samples were placed on the upper back of participants, and closed for 4, 24 or 48 h. A patch application time of 24 h is sufficient to detect primary skin irritation from irritants in cosmetics and other common household products. In addition, we found that skin irritation reactions were strongest at 24 h after patch removal and that the reaction tended to be weaker at 48 h after patch removal. Patch testing to evaluate irritants should be performed by means of a 24-h patch test with a follow-up reading at 24 h after patch removal. An application time of 24 h places less of a burden on patients than a 48-h patch test.
AB - We investigated the optimum application for evaluating skin irritation response by using samples of irritants commonly used as additives in cosmetics and other common household products. We studied 47 volunteers (16 men and 31 women). We selected three types of surfactant, one moisturizer, one anti-infective agent and one oil solution. Using Finn chambers on Scanpor tape, we performed the patch test. A total of 0.015 mL of each sample was applied to the Finn chamber. For liquids, circular filter paper was soaked in 0.015 mL of the sample. Samples were placed on the upper back of participants, and closed for 4, 24 or 48 h. A patch application time of 24 h is sufficient to detect primary skin irritation from irritants in cosmetics and other common household products. In addition, we found that skin irritation reactions were strongest at 24 h after patch removal and that the reaction tended to be weaker at 48 h after patch removal. Patch testing to evaluate irritants should be performed by means of a 24-h patch test with a follow-up reading at 24 h after patch removal. An application time of 24 h places less of a burden on patients than a 48-h patch test.
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U2 - 10.1111/1346-8138.12004
DO - 10.1111/1346-8138.12004
M3 - Article
C2 - 23414058
AN - SCOPUS:84877740482
SN - 0385-2407
VL - 40
SP - 363
EP - 369
JO - Journal of Dermatology
JF - Journal of Dermatology
IS - 5
ER -