Oral L-citrulline supplementation improves erectile function and penile structure in castrated rats

Yuji Hotta, Arufumi Shiota, Tomoya Kataoka, Marina Motonari, Yasuhiro Maeda, Masahiko Morita, Kazunori Kimura

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9 Citations (Scopus)

Abstract

Objectives: To investigate the efficacy of oral l-citrulline for erectile dysfunction and penile structure disruption in a rat model. Methods: Male Wistar-ST rats aged 15 weeks were randomly divided into three groups as follows: sham-operated rats (control group), surgically castrated rats (castrated group) and surgically castrated rats subsequently treated with 2% l-citrulline water (castrated+l-citrulline). At 4 weeks postoperative, erectile function was assessed based on intracavernous pressure changes, followed by electrostimulation of cavernous nerves and calculation of maximum intracavernous pressure/mean arterial pressure. Penile structure was evaluated by Masson's trichrome staining and the smooth muscle-to-collagen ratio was calculated. The serum bioavailable testosterone, l-arginine, l-citrulline, NG,NG-dimethylarginine and nitrogen oxide levels were evaluated. Results: The bioavailable testosterone concentrations were decreased in the castrated and castrated+l-citrulline groups compared with the control group at 4 weeks after surgery. The intracavernous pressure-to-mean arterial pressure and smooth muscle-to-collagen ratios were significantly decreased in the castrated group compared with the control group, but significantly increased in the castrated+l-citrulline group compared with the castrated group. The serum l-citrulline, l-arginine and NG,NG-dimethylarginine levels, and the l-arginine-to-NG,NG-dimethylarginine ratios were significantly increased in the castrated+l-citrulline group compared with the castrated group. The serum nitrogen oxide levels were increased in the castrated+l-citrulline group compared with the castrated group. Conclusions: Oral l-citrulline can improve the erectile response to electric stimulation of cavernous nerve and penile structure in castrated rats.

Original languageEnglish
Pages (from-to)608-612
Number of pages5
JournalInternational Journal of Urology
Volume21
Issue number6
DOIs
Publication statusPublished - 06-2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Urology

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    Hotta, Y., Shiota, A., Kataoka, T., Motonari, M., Maeda, Y., Morita, M., & Kimura, K. (2014). Oral L-citrulline supplementation improves erectile function and penile structure in castrated rats. International Journal of Urology, 21(6), 608-612. https://doi.org/10.1111/iju.12362