@inbook{623b22cf1cdc41babca4d2fd6f9371b7,
title = "Organoid culture of human cancer stem cells",
abstract = "Organoid culture is a three-dimensional culture method that enables ex vivo analysis of stem cell behavior and differentiation. This method is also applicable to the studies on stem cell characters of human cancer stem cells. The components of organoid culture include Matrigel{\textregistered} and a culture medium containing growth factor cocktails that mimic the microenvironments of organ stem cell niches. Here, we describe the basic methods for the organoid culture of dissociated or FACS-sorted human cancer stem cells. Then, we introduce a method to dissociate the organoids for serial passage and propagation.",
author = "Yohei Shimono and Junko Mukohyama and Taichi Isobe and Johnston, {Darius M.} and Piero Dalerba and Akira Suzuki",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2019",
doi = "10.1007/7651_2016_13",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "23--31",
booktitle = "Methods in Molecular Biology",
}
