Orthotopic lung cancer murine model by nonoperative transbronchial approach

Takahiro Nakajima, Takashi Anayama, Yasushi Matsuda, David M. Hwang, Patrick Z. McVeigh, Brian C. Wilson, Gang Zheng, Shaf Keshavjee, Kazuhiro Yasufuku

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose The aim of this work was to establish a novel orthotopic human non-small cell lung cancer (NSCLC) murine xenograft model by a nonsurgical, transbronchial approach. Description Male athymic nude mice and human NSCLC cell lines, including A549, H460, and H520 were used. Under direct visualization of the vocal cords, a 23-gauge blunt-tip slightly curved metal catheter was introduced into the trachea to the bronchus, and 2.5 × 105 tumor cells mixed with Matrigel (BD Biosciences, Mississauga, Ontario, Canada) were administered into the lung. Mice were monitored using weekly microcomputed tomography scans for tumor formation. Evaluation When the tumor size reached more than 4 mm in diameter, the animals were euthanized, and the tumor tissue was evaluated histopathologically. Of 37 mice studied, 34 were confirmed to have tumor formation: 29 developed solitary tumors and 5 had multifocal lesions. There was no evidence of extrapleural dissemination or effusion. Conclusions Transbronchial delivery of tumor cells enabled the establishment of a novel orthotopic human NSCLC murine xenograft model. This clinically relevant preclinical model bearing a solitary nodule is of value for a variety of in vivo research studies.

Original languageEnglish
Pages (from-to)1771-1775
Number of pages5
JournalAnnals of Thoracic Surgery
Volume97
Issue number5
DOIs
Publication statusPublished - 05-2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Nakajima, T., Anayama, T., Matsuda, Y., Hwang, D. M., McVeigh, P. Z., Wilson, B. C., Zheng, G., Keshavjee, S., & Yasufuku, K. (2014). Orthotopic lung cancer murine model by nonoperative transbronchial approach. Annals of Thoracic Surgery, 97(5), 1771-1775. https://doi.org/10.1016/j.athoracsur.2014.01.048