TY - JOUR
T1 - Orthotopic lung cancer murine model by nonoperative transbronchial approach
AU - Nakajima, Takahiro
AU - Anayama, Takashi
AU - Matsuda, Yasushi
AU - Hwang, David M.
AU - McVeigh, Patrick Z.
AU - Wilson, Brian C.
AU - Zheng, Gang
AU - Keshavjee, Shaf
AU - Yasufuku, Kazuhiro
N1 - Funding Information:
This work was supported by Canadian Institutes of Health Research (CIHR No. 111623 and No. 119597). T.N. received a research fellowship from the Uehara Memorial Foundation and Kanae Foundation for the Promotion of Medical Science. These sponsors had no role in the study design, conduct of the study, data collection, data management and interpretation, preparation of the report, review of the report, or approval of the report. Infrastructure support was provided by the Ontario Ministry of Health and Long-Term Care (OMOHLTC), which does not necessarily endorse the views expressed in this paper.
PY - 2014/5
Y1 - 2014/5
N2 - Purpose The aim of this work was to establish a novel orthotopic human non-small cell lung cancer (NSCLC) murine xenograft model by a nonsurgical, transbronchial approach. Description Male athymic nude mice and human NSCLC cell lines, including A549, H460, and H520 were used. Under direct visualization of the vocal cords, a 23-gauge blunt-tip slightly curved metal catheter was introduced into the trachea to the bronchus, and 2.5 × 105 tumor cells mixed with Matrigel (BD Biosciences, Mississauga, Ontario, Canada) were administered into the lung. Mice were monitored using weekly microcomputed tomography scans for tumor formation. Evaluation When the tumor size reached more than 4 mm in diameter, the animals were euthanized, and the tumor tissue was evaluated histopathologically. Of 37 mice studied, 34 were confirmed to have tumor formation: 29 developed solitary tumors and 5 had multifocal lesions. There was no evidence of extrapleural dissemination or effusion. Conclusions Transbronchial delivery of tumor cells enabled the establishment of a novel orthotopic human NSCLC murine xenograft model. This clinically relevant preclinical model bearing a solitary nodule is of value for a variety of in vivo research studies.
AB - Purpose The aim of this work was to establish a novel orthotopic human non-small cell lung cancer (NSCLC) murine xenograft model by a nonsurgical, transbronchial approach. Description Male athymic nude mice and human NSCLC cell lines, including A549, H460, and H520 were used. Under direct visualization of the vocal cords, a 23-gauge blunt-tip slightly curved metal catheter was introduced into the trachea to the bronchus, and 2.5 × 105 tumor cells mixed with Matrigel (BD Biosciences, Mississauga, Ontario, Canada) were administered into the lung. Mice were monitored using weekly microcomputed tomography scans for tumor formation. Evaluation When the tumor size reached more than 4 mm in diameter, the animals were euthanized, and the tumor tissue was evaluated histopathologically. Of 37 mice studied, 34 were confirmed to have tumor formation: 29 developed solitary tumors and 5 had multifocal lesions. There was no evidence of extrapleural dissemination or effusion. Conclusions Transbronchial delivery of tumor cells enabled the establishment of a novel orthotopic human NSCLC murine xenograft model. This clinically relevant preclinical model bearing a solitary nodule is of value for a variety of in vivo research studies.
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U2 - 10.1016/j.athoracsur.2014.01.048
DO - 10.1016/j.athoracsur.2014.01.048
M3 - Article
C2 - 24792261
AN - SCOPUS:84899869256
SN - 0003-4975
VL - 97
SP - 1771
EP - 1775
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 5
ER -
