Osteoclastic activity induces osteomodulin expression in osteoblasts

Ken Ninomiya, Takeshi Miyamoto, Jun ichi Imai, Nobuyuki Fujita, Toru Suzuki, Ryotaro Iwasaki, Mitsuru Yagi, Shinya Watanabe, Yoshiaki Toyama, Toshio Suda

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27 Citations (Scopus)


Bone resorption by osteoclasts stimulates bone formation by osteoblasts. To isolate osteoblastic factors coupled with osteoclast activity, we performed microarray and cluster analysis of 8 tissues including bone, and found that among 10,490 genes, osteomodulin (OMD), an extracellular matrix keratan sulfate proteoglycan, was simultaneously induced with osteoclast-specific markers such as MMP9 and Acp5. OMD expression was detected in osteoblasts and upregulated during osteoblast maturation. OMD expression in osteoblasts was also detected immunohistochemically using a specific antibody against OMD. The immunoreactivity against OMD decreased in op/op mice, which lack functional macrophage colony stimulating factor (M-CSF) and are therefore defective in osteoclast formation, when compared to wild-type littermates. OMD expression in op/op mice was upregulated by M-CSF treatment. Since the M-CSF receptor c-Fms was not expressed in osteoblasts, it is likely that OMD is an osteoblast maturation marker that is induced by osteoclast activity.

Original languageEnglish
Pages (from-to)460-466
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 19-10-2007

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Ninomiya, K., Miyamoto, T., Imai, J. I., Fujita, N., Suzuki, T., Iwasaki, R., Yagi, M., Watanabe, S., Toyama, Y., & Suda, T. (2007). Osteoclastic activity induces osteomodulin expression in osteoblasts. Biochemical and Biophysical Research Communications, 362(2), 460-466. https://doi.org/10.1016/j.bbrc.2007.07.193