Osteoclastic activity induces osteomodulin expression in osteoblasts

Ken Ninomiya, Takeshi Miyamoto, Jun ichi Imai, Nobuyuki Fujita, Toru Suzuki, Ryotaro Iwasaki, Mitsuru Yagi, Shinya Watanabe, Yoshiaki Toyama, Toshio Suda

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Bone resorption by osteoclasts stimulates bone formation by osteoblasts. To isolate osteoblastic factors coupled with osteoclast activity, we performed microarray and cluster analysis of 8 tissues including bone, and found that among 10,490 genes, osteomodulin (OMD), an extracellular matrix keratan sulfate proteoglycan, was simultaneously induced with osteoclast-specific markers such as MMP9 and Acp5. OMD expression was detected in osteoblasts and upregulated during osteoblast maturation. OMD expression in osteoblasts was also detected immunohistochemically using a specific antibody against OMD. The immunoreactivity against OMD decreased in op/op mice, which lack functional macrophage colony stimulating factor (M-CSF) and are therefore defective in osteoclast formation, when compared to wild-type littermates. OMD expression in op/op mice was upregulated by M-CSF treatment. Since the M-CSF receptor c-Fms was not expressed in osteoblasts, it is likely that OMD is an osteoblast maturation marker that is induced by osteoclast activity.

Original languageEnglish
Pages (from-to)460-466
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume362
Issue number2
DOIs
Publication statusPublished - 19-10-2007

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Ninomiya, K., Miyamoto, T., Imai, J. I., Fujita, N., Suzuki, T., Iwasaki, R., Yagi, M., Watanabe, S., Toyama, Y., & Suda, T. (2007). Osteoclastic activity induces osteomodulin expression in osteoblasts. Biochemical and Biophysical Research Communications, 362(2), 460-466. https://doi.org/10.1016/j.bbrc.2007.07.193