Overexpression of IgLON cell adhesion molecules changes proliferation and cell size of cortical astrocytes

Chiaki Sugimoto; Shoko Morita; Seiji Miyata

doi:10.1002/cbf.2813

Overexpression of IgLON cell adhesion molecules changes proliferation and cell size of cortical astrocytes

Chiaki Sugimoto
, Shoko Morita
, Seiji Miyata

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

IgLON family is a subgroup of the immunoglobulin superfamily cell adhesion molecules and composed of limbic system-associated protein (LAMP), opioid binding cell adhesion molecule (OBCAM), neurotrimin (Ntm) and Kilon. In the present study, we investigated the overexpression of LAMP, OBCAM, Ntm and Kilon on the proliferation and cell size of type-1 astrocytes in vitro. Quantitative analysis using bromodeoxyuridine immunocytochemistry revealed that the expression of OBCAM had greater inhibitory effect on astrocytic proliferation as compared with LAMP, Ntm and Kilon ones. OBCAM overexpression increased the cell size of astrocytes as compared with the control. The treatment of FGF-2 had greater proliferative effect on OBCAM-transfected astrocytes as compared with the control. These results suggest that OBCAM is more potent regulator for controlling the proliferation and cell size of astrocytes as compared with other IgLON proteins possibly through FGF-2 receptor-mediated pathway.

Original language	English
Pages (from-to)	400-405
Number of pages	6
Journal	Cell Biochemistry and Function
Volume	30
Issue number	5
DOIs	https://doi.org/10.1002/cbf.2813
Publication status	Published - 07-2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Clinical Biochemistry
Cell Biology

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10.1002/cbf.2813

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title = "Overexpression of IgLON cell adhesion molecules changes proliferation and cell size of cortical astrocytes",

abstract = "IgLON family is a subgroup of the immunoglobulin superfamily cell adhesion molecules and composed of limbic system-associated protein (LAMP), opioid binding cell adhesion molecule (OBCAM), neurotrimin (Ntm) and Kilon. In the present study, we investigated the overexpression of LAMP, OBCAM, Ntm and Kilon on the proliferation and cell size of type-1 astrocytes in vitro. Quantitative analysis using bromodeoxyuridine immunocytochemistry revealed that the expression of OBCAM had greater inhibitory effect on astrocytic proliferation as compared with LAMP, Ntm and Kilon ones. OBCAM overexpression increased the cell size of astrocytes as compared with the control. The treatment of FGF-2 had greater proliferative effect on OBCAM-transfected astrocytes as compared with the control. These results suggest that OBCAM is more potent regulator for controlling the proliferation and cell size of astrocytes as compared with other IgLON proteins possibly through FGF-2 receptor-mediated pathway.",

keywords = "Astrocyte, Cell adhesion molecule, FGF-2, Kilon, LAMP, Neurotrimin, Proliferation",

author = "Chiaki Sugimoto and Shoko Morita and Seiji Miyata",

year = "2012",

month = jul,

doi = "10.1002/cbf.2813",

language = "English",

volume = "30",

pages = "400--405",

journal = "Cell Biochemistry and Function",

issn = "0263-6484",

publisher = "John Wiley and Sons Ltd",

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TY - JOUR

T1 - Overexpression of IgLON cell adhesion molecules changes proliferation and cell size of cortical astrocytes

AU - Sugimoto, Chiaki

AU - Morita, Shoko

AU - Miyata, Seiji

PY - 2012/7

Y1 - 2012/7

N2 - IgLON family is a subgroup of the immunoglobulin superfamily cell adhesion molecules and composed of limbic system-associated protein (LAMP), opioid binding cell adhesion molecule (OBCAM), neurotrimin (Ntm) and Kilon. In the present study, we investigated the overexpression of LAMP, OBCAM, Ntm and Kilon on the proliferation and cell size of type-1 astrocytes in vitro. Quantitative analysis using bromodeoxyuridine immunocytochemistry revealed that the expression of OBCAM had greater inhibitory effect on astrocytic proliferation as compared with LAMP, Ntm and Kilon ones. OBCAM overexpression increased the cell size of astrocytes as compared with the control. The treatment of FGF-2 had greater proliferative effect on OBCAM-transfected astrocytes as compared with the control. These results suggest that OBCAM is more potent regulator for controlling the proliferation and cell size of astrocytes as compared with other IgLON proteins possibly through FGF-2 receptor-mediated pathway.

AB - IgLON family is a subgroup of the immunoglobulin superfamily cell adhesion molecules and composed of limbic system-associated protein (LAMP), opioid binding cell adhesion molecule (OBCAM), neurotrimin (Ntm) and Kilon. In the present study, we investigated the overexpression of LAMP, OBCAM, Ntm and Kilon on the proliferation and cell size of type-1 astrocytes in vitro. Quantitative analysis using bromodeoxyuridine immunocytochemistry revealed that the expression of OBCAM had greater inhibitory effect on astrocytic proliferation as compared with LAMP, Ntm and Kilon ones. OBCAM overexpression increased the cell size of astrocytes as compared with the control. The treatment of FGF-2 had greater proliferative effect on OBCAM-transfected astrocytes as compared with the control. These results suggest that OBCAM is more potent regulator for controlling the proliferation and cell size of astrocytes as compared with other IgLON proteins possibly through FGF-2 receptor-mediated pathway.

KW - Astrocyte

KW - Cell adhesion molecule

KW - FGF-2

KW - Kilon

KW - LAMP

KW - Neurotrimin

KW - Proliferation

UR - https://www.scopus.com/pages/publications/84863316850

UR - https://www.scopus.com/pages/publications/84863316850#tab=citedBy

U2 - 10.1002/cbf.2813

DO - 10.1002/cbf.2813

M3 - Article

C2 - 22374746

AN - SCOPUS:84863316850

SN - 0263-6484

VL - 30

SP - 400

EP - 405

JO - Cell Biochemistry and Function

JF - Cell Biochemistry and Function

IS - 5

ER -

Overexpression of IgLON cell adhesion molecules changes proliferation and cell size of cortical astrocytes

Abstract

All Science Journal Classification (ASJC) codes

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