Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells

Fang Cao, Ryuji Hata, Pengxiang Zhu, Yong Jie Ma, Junya Tanaka, Yasushi Hanakawa, Koji Hashimoto, Michio Niinobe, Kazuaki Yoshikawa, Masahiro Sakanaka

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

To investigate the effects of suppressors of cytokine signaling 3 (SOCS3) on neural stem cell fate, stem cells were infected with an adenoviral vector expressing SOCS3. Three days later, western blot analysis and immunocytochemical analysis revealed that the protein level of MAP2 and the number of MAP2-positive cells were significantly increased in SOCS3-transfected cells, whereas the protein level of GFAP and the number of GFAP-positive cells were significantly decreased. Furthermore, promoter assay revealed a significant reduction in the transcriptional level of signal transducer and activator of transcription 3 (Stat3) in the transfected cells. In addition, the mRNA levels of Notch family member (notch1) and inhibitory basic helix-loop-helix (bHLH) factors (hes5 and id3) were significantly up-regulated 1 day after overexpression of SOCS3. Three days after transfection, the mRNA level of hes5 was significantly decreased, whereas that of notch1 was still up-regulated. Moreover, all of SOCS3-positive cells expressed Nestin protein but did not express MAP2 or GFAP proteins. These data indicate that overexpression of SOCS3 induced neurogenesis and inhibited astrogliogenesis in neural stem cells. Our data also show that SOCS3 promoted maintenance of neural stem cells.

Original languageEnglish
Pages (from-to)459-470
Number of pages12
JournalJournal of Neurochemistry
Volume98
Issue number2
DOIs
Publication statusPublished - 01-07-2006

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Neural Stem Cells
Stem cells
Maintenance
Cytokines
Cells
Nestin
STAT3 Transcription Factor
Messenger RNA
Proteins
Neurogenesis
Transfection
Assays
Stem Cells
Western Blotting

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Cao, Fang ; Hata, Ryuji ; Zhu, Pengxiang ; Ma, Yong Jie ; Tanaka, Junya ; Hanakawa, Yasushi ; Hashimoto, Koji ; Niinobe, Michio ; Yoshikawa, Kazuaki ; Sakanaka, Masahiro. / Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells. In: Journal of Neurochemistry. 2006 ; Vol. 98, No. 2. pp. 459-470.
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abstract = "To investigate the effects of suppressors of cytokine signaling 3 (SOCS3) on neural stem cell fate, stem cells were infected with an adenoviral vector expressing SOCS3. Three days later, western blot analysis and immunocytochemical analysis revealed that the protein level of MAP2 and the number of MAP2-positive cells were significantly increased in SOCS3-transfected cells, whereas the protein level of GFAP and the number of GFAP-positive cells were significantly decreased. Furthermore, promoter assay revealed a significant reduction in the transcriptional level of signal transducer and activator of transcription 3 (Stat3) in the transfected cells. In addition, the mRNA levels of Notch family member (notch1) and inhibitory basic helix-loop-helix (bHLH) factors (hes5 and id3) were significantly up-regulated 1 day after overexpression of SOCS3. Three days after transfection, the mRNA level of hes5 was significantly decreased, whereas that of notch1 was still up-regulated. Moreover, all of SOCS3-positive cells expressed Nestin protein but did not express MAP2 or GFAP proteins. These data indicate that overexpression of SOCS3 induced neurogenesis and inhibited astrogliogenesis in neural stem cells. Our data also show that SOCS3 promoted maintenance of neural stem cells.",
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Cao, F, Hata, R, Zhu, P, Ma, YJ, Tanaka, J, Hanakawa, Y, Hashimoto, K, Niinobe, M, Yoshikawa, K & Sakanaka, M 2006, 'Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells', Journal of Neurochemistry, vol. 98, no. 2, pp. 459-470. https://doi.org/10.1111/j.1471-4159.2006.03890.x

Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells. / Cao, Fang; Hata, Ryuji; Zhu, Pengxiang; Ma, Yong Jie; Tanaka, Junya; Hanakawa, Yasushi; Hashimoto, Koji; Niinobe, Michio; Yoshikawa, Kazuaki; Sakanaka, Masahiro.

In: Journal of Neurochemistry, Vol. 98, No. 2, 01.07.2006, p. 459-470.

Research output: Contribution to journalArticle

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T1 - Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells

AU - Cao, Fang

AU - Hata, Ryuji

AU - Zhu, Pengxiang

AU - Ma, Yong Jie

AU - Tanaka, Junya

AU - Hanakawa, Yasushi

AU - Hashimoto, Koji

AU - Niinobe, Michio

AU - Yoshikawa, Kazuaki

AU - Sakanaka, Masahiro

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