OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois

Janak Koirala; Isha Tyagi; Lohitha Guntupalli; Sameena Koirala; Udita Chapagain; Christopher Quarshie; Sami Akram; Vidya Sundareshan; Sajan Koirala; Jerry Lawhorn; Yohei Doi; Michael Olson

doi:10.1016/j.diagmicrobio.2020.114999

OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois

Janak Koirala, Isha Tyagi, Lohitha Guntupalli, Sameena Koirala, Udita Chapagain, Christopher Quarshie, Sami Akram, Vidya Sundareshan, Sajan Koirala, Jerry Lawhorn, Yohei Doi, Michael Olson

Research output: Contribution to journal › Article › peer-review

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Abstract

We reviewed susceptibility of 840 A. baumannii complex isolates at two academic medical centers and explored their mechanism of carbapenem resistance. Carbapenem resistance rates among A. baumannii increased from <5% before 2005 to 55% in 2011 and declined thereafter. We subjected 86 isolates for further antibiotic susceptibility testing using E-test, screened for MBL and carbapenemase production, and performed PCR for bla_OXA genes. Statistical analyses included correlation of resistance genes with susceptibility. Sixty-one isolates were non-susceptible to carbapenems (MIC >2 μg/mL). Phenotypic screening showed carbapenemase production in 50 isolates, but none was positive for MBL. Among carbapenem non-susceptible isolates, the CHDL (group D carbapenemase) encoding genes bla_OXA-23 (52%) and bla_OXA-40 (28%) were the most frequent genes. In conclusion, carbapenem resistance rates in A. baumannii peaked in 2011 and have since declined in our region. Carbapenem resistance among A. baumannii was primarily associated with production of acquired CHDLs including OXA-23 and OXA-40.

Original language	English
Article number	114999
Journal	Diagnostic Microbiology and Infectious Disease
Volume	97
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2020.114999
Publication status	Published - 05-2020
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2020.114999

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Koirala, J., Tyagi, I., Guntupalli, L., Koirala, S., Chapagain, U., Quarshie, C., Akram, S., Sundareshan, V., Koirala, S., Lawhorn, J., Doi, Y., & Olson, M. (2020). OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois. Diagnostic Microbiology and Infectious Disease, 97(1), Article 114999. https://doi.org/10.1016/j.diagmicrobio.2020.114999

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title = "OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois",

abstract = "We reviewed susceptibility of 840 A. baumannii complex isolates at two academic medical centers and explored their mechanism of carbapenem resistance. Carbapenem resistance rates among A. baumannii increased from <5% before 2005 to 55% in 2011 and declined thereafter. We subjected 86 isolates for further antibiotic susceptibility testing using E-test, screened for MBL and carbapenemase production, and performed PCR for blaOXA genes. Statistical analyses included correlation of resistance genes with susceptibility. Sixty-one isolates were non-susceptible to carbapenems (MIC >2 μg/mL). Phenotypic screening showed carbapenemase production in 50 isolates, but none was positive for MBL. Among carbapenem non-susceptible isolates, the CHDL (group D carbapenemase) encoding genes blaOXA-23 (52%) and blaOXA-40 (28%) were the most frequent genes. In conclusion, carbapenem resistance rates in A. baumannii peaked in 2011 and have since declined in our region. Carbapenem resistance among A. baumannii was primarily associated with production of acquired CHDLs including OXA-23 and OXA-40.",

keywords = "Acinetobacter baumannii, CHDL, Carbapenem resistance, OXA-23, OXA-40, Resistance mechanism",

author = "Janak Koirala and Isha Tyagi and Lohitha Guntupalli and Sameena Koirala and Udita Chapagain and Christopher Quarshie and Sami Akram and Vidya Sundareshan and Sajan Koirala and Jerry Lawhorn and Yohei Doi and Michael Olson",

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doi = "10.1016/j.diagmicrobio.2020.114999",

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T1 - OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois

AU - Koirala, Janak

AU - Tyagi, Isha

AU - Guntupalli, Lohitha

AU - Koirala, Sameena

AU - Chapagain, Udita

AU - Quarshie, Christopher

AU - Akram, Sami

AU - Sundareshan, Vidya

AU - Koirala, Sajan

AU - Lawhorn, Jerry

AU - Doi, Yohei

AU - Olson, Michael

PY - 2020/5

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N2 - We reviewed susceptibility of 840 A. baumannii complex isolates at two academic medical centers and explored their mechanism of carbapenem resistance. Carbapenem resistance rates among A. baumannii increased from <5% before 2005 to 55% in 2011 and declined thereafter. We subjected 86 isolates for further antibiotic susceptibility testing using E-test, screened for MBL and carbapenemase production, and performed PCR for blaOXA genes. Statistical analyses included correlation of resistance genes with susceptibility. Sixty-one isolates were non-susceptible to carbapenems (MIC >2 μg/mL). Phenotypic screening showed carbapenemase production in 50 isolates, but none was positive for MBL. Among carbapenem non-susceptible isolates, the CHDL (group D carbapenemase) encoding genes blaOXA-23 (52%) and blaOXA-40 (28%) were the most frequent genes. In conclusion, carbapenem resistance rates in A. baumannii peaked in 2011 and have since declined in our region. Carbapenem resistance among A. baumannii was primarily associated with production of acquired CHDLs including OXA-23 and OXA-40.

AB - We reviewed susceptibility of 840 A. baumannii complex isolates at two academic medical centers and explored their mechanism of carbapenem resistance. Carbapenem resistance rates among A. baumannii increased from <5% before 2005 to 55% in 2011 and declined thereafter. We subjected 86 isolates for further antibiotic susceptibility testing using E-test, screened for MBL and carbapenemase production, and performed PCR for blaOXA genes. Statistical analyses included correlation of resistance genes with susceptibility. Sixty-one isolates were non-susceptible to carbapenems (MIC >2 μg/mL). Phenotypic screening showed carbapenemase production in 50 isolates, but none was positive for MBL. Among carbapenem non-susceptible isolates, the CHDL (group D carbapenemase) encoding genes blaOXA-23 (52%) and blaOXA-40 (28%) were the most frequent genes. In conclusion, carbapenem resistance rates in A. baumannii peaked in 2011 and have since declined in our region. Carbapenem resistance among A. baumannii was primarily associated with production of acquired CHDLs including OXA-23 and OXA-40.

KW - Acinetobacter baumannii

KW - CHDL

KW - Carbapenem resistance

KW - OXA-23

KW - OXA-40

KW - Resistance mechanism

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OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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