Oxiradical-dependent photoemission induced by a phacoemulsification probe

S. Shimmura, K. Tsubota, Y. Oguchi, D. Fukumura, M. Suematsu, M. Tsuchiya

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58 Citations (Scopus)


Oxygen free radical formation by conventional phacoemulsification devices has been postulated as a possible mechanism of corneal endothelial damage during surgery. To test this hypothesis, phacoemulsification probe-induced free radical production was visualized using a single photon-counting camera and an O2--sensitive luciferin derivative, 2-methyl-6-[p-methoxyphenyl-3,7- dihydroimidazo [1,2-a]pyrazin-3-one (MCLA), which allows the visualization of spatial and temporal alterations in free radical production. Within 1 min after starting ultrasound emission, MCLA-dependent chemiluminescence was increased significantly, the intensity of which was maximal at the tip of the probe and tapered along a gradient toward distal portions. The chemiluminescence was suppressed significantly by adding either superoxide dismutase (300 U/ml) or sodium azide (20 mmol/l). By adding deuterium to the medium, MCLA-dependent chemiluminescence significantly increased, suggesting the involvement of singlet oxygen in the reaction.

Original languageEnglish
Pages (from-to)2904-2907
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Issue number10
Publication statusPublished - 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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