P-selectin-dependent macrophage migration into the tubulointerstitium in unilateral ureteral obstruction

Tomohiko Naruse, Yukio Yuzawa, Toshiyuki Akahori, Masashi Mizuno, Shoji Maruyama, Reiji Kannagi, Nigishi Hotta, Seiichi Matsuo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background. Interstitial infiltration of macrophages (Mø) is one of the main causal factors for the tubulointerstitial injury. However, precise mechanisms of Mø infiltration into tubulointerstitium have not been fully explored. The purposes of this study were to assess the role of selectins in the acute infiltration of Mø in rats with unilateral ureteral obstruction (UUO) and to evaluate the role of vasa recta, that is, whether they facilitate massive influx of Mø into the interstitium by functioning as specialized vessels. Methods. To evaluate the role of selectins in Mø infiltration into tubulointerstitium, the expression of selectins and L-selectin ligands was examined by immunohistochemistry and immunoelectron microscopy. The functional role of P-selectin in vasa recta was studied by Stamper-Woodruff assay, in vivo p-Mø migration assay and in vivo blocking experiments with the monoclonal antibody (mAb) ARP2-4. Results. Selective expression of P-selectin was detected in vasa recta as early as one hour after UUO, and the expression increased thereafter for 96 hours. In contrast, endothelial expression of L-selectin ligands and E-selectin were not detectable. In the Stamper-Woodruff assay on kidney sections of rats with UUO, the adhesion of isolated rat peritoneal Mø (p-Mø) to vasa recta was significantly inhibited by the mAb ARP2-4 (P-selectin blocker; P < 0.01), but not by mAb ARE-5 (E-selectin blocker) or rLECIg (rat L-selectin chimeric protein). In the in vivo transfer experiments with fluorescein-labeled p-Mø into rats 48 hours after UUO, labeled p-Mø had accumulated around vasa recta at three minutes and had infiltrated predominantly into the outer medulla at 180 minutes. The number of labeled p-Mø was reduced when the rats were pretreated with ARP2-4 (P < 0.01). Finally, ARP2-4 (10 mg/kg), injected 15 minutes before UUO, reduced the number of infiltrated Mø (P < 0.01). Conclusion. The results suggest that vasa recta, which express P-selectin, contribute to massive infiltration of Mø into the interstitium by functioning as specialized post-capillary venules.

Original languageEnglish
Pages (from-to)94-105
Number of pages12
JournalKidney International
Volume62
Issue number1
DOIs
Publication statusPublished - 01-01-2002
Externally publishedYes

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Ureteral Obstruction
P-Selectin
Rectum
Macrophages
L-Selectin
Selectins
E-Selectin
Monoclonal Antibodies
Ligands
Venules
Immunoelectron Microscopy
Fluorescein
Immunohistochemistry
Kidney
Wounds and Injuries
Proteins

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Naruse, Tomohiko ; Yuzawa, Yukio ; Akahori, Toshiyuki ; Mizuno, Masashi ; Maruyama, Shoji ; Kannagi, Reiji ; Hotta, Nigishi ; Matsuo, Seiichi. / P-selectin-dependent macrophage migration into the tubulointerstitium in unilateral ureteral obstruction. In: Kidney International. 2002 ; Vol. 62, No. 1. pp. 94-105.
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title = "P-selectin-dependent macrophage migration into the tubulointerstitium in unilateral ureteral obstruction",
abstract = "Background. Interstitial infiltration of macrophages (M{\o}) is one of the main causal factors for the tubulointerstitial injury. However, precise mechanisms of M{\o} infiltration into tubulointerstitium have not been fully explored. The purposes of this study were to assess the role of selectins in the acute infiltration of M{\o} in rats with unilateral ureteral obstruction (UUO) and to evaluate the role of vasa recta, that is, whether they facilitate massive influx of M{\o} into the interstitium by functioning as specialized vessels. Methods. To evaluate the role of selectins in M{\o} infiltration into tubulointerstitium, the expression of selectins and L-selectin ligands was examined by immunohistochemistry and immunoelectron microscopy. The functional role of P-selectin in vasa recta was studied by Stamper-Woodruff assay, in vivo p-M{\o} migration assay and in vivo blocking experiments with the monoclonal antibody (mAb) ARP2-4. Results. Selective expression of P-selectin was detected in vasa recta as early as one hour after UUO, and the expression increased thereafter for 96 hours. In contrast, endothelial expression of L-selectin ligands and E-selectin were not detectable. In the Stamper-Woodruff assay on kidney sections of rats with UUO, the adhesion of isolated rat peritoneal M{\o} (p-M{\o}) to vasa recta was significantly inhibited by the mAb ARP2-4 (P-selectin blocker; P < 0.01), but not by mAb ARE-5 (E-selectin blocker) or rLECIg (rat L-selectin chimeric protein). In the in vivo transfer experiments with fluorescein-labeled p-M{\o} into rats 48 hours after UUO, labeled p-M{\o} had accumulated around vasa recta at three minutes and had infiltrated predominantly into the outer medulla at 180 minutes. The number of labeled p-M{\o} was reduced when the rats were pretreated with ARP2-4 (P < 0.01). Finally, ARP2-4 (10 mg/kg), injected 15 minutes before UUO, reduced the number of infiltrated M{\o} (P < 0.01). Conclusion. The results suggest that vasa recta, which express P-selectin, contribute to massive infiltration of M{\o} into the interstitium by functioning as specialized post-capillary venules.",
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P-selectin-dependent macrophage migration into the tubulointerstitium in unilateral ureteral obstruction. / Naruse, Tomohiko; Yuzawa, Yukio; Akahori, Toshiyuki; Mizuno, Masashi; Maruyama, Shoji; Kannagi, Reiji; Hotta, Nigishi; Matsuo, Seiichi.

In: Kidney International, Vol. 62, No. 1, 01.01.2002, p. 94-105.

Research output: Contribution to journalArticle

TY - JOUR

T1 - P-selectin-dependent macrophage migration into the tubulointerstitium in unilateral ureteral obstruction

AU - Naruse, Tomohiko

AU - Yuzawa, Yukio

AU - Akahori, Toshiyuki

AU - Mizuno, Masashi

AU - Maruyama, Shoji

AU - Kannagi, Reiji

AU - Hotta, Nigishi

AU - Matsuo, Seiichi

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Background. Interstitial infiltration of macrophages (Mø) is one of the main causal factors for the tubulointerstitial injury. However, precise mechanisms of Mø infiltration into tubulointerstitium have not been fully explored. The purposes of this study were to assess the role of selectins in the acute infiltration of Mø in rats with unilateral ureteral obstruction (UUO) and to evaluate the role of vasa recta, that is, whether they facilitate massive influx of Mø into the interstitium by functioning as specialized vessels. Methods. To evaluate the role of selectins in Mø infiltration into tubulointerstitium, the expression of selectins and L-selectin ligands was examined by immunohistochemistry and immunoelectron microscopy. The functional role of P-selectin in vasa recta was studied by Stamper-Woodruff assay, in vivo p-Mø migration assay and in vivo blocking experiments with the monoclonal antibody (mAb) ARP2-4. Results. Selective expression of P-selectin was detected in vasa recta as early as one hour after UUO, and the expression increased thereafter for 96 hours. In contrast, endothelial expression of L-selectin ligands and E-selectin were not detectable. In the Stamper-Woodruff assay on kidney sections of rats with UUO, the adhesion of isolated rat peritoneal Mø (p-Mø) to vasa recta was significantly inhibited by the mAb ARP2-4 (P-selectin blocker; P < 0.01), but not by mAb ARE-5 (E-selectin blocker) or rLECIg (rat L-selectin chimeric protein). In the in vivo transfer experiments with fluorescein-labeled p-Mø into rats 48 hours after UUO, labeled p-Mø had accumulated around vasa recta at three minutes and had infiltrated predominantly into the outer medulla at 180 minutes. The number of labeled p-Mø was reduced when the rats were pretreated with ARP2-4 (P < 0.01). Finally, ARP2-4 (10 mg/kg), injected 15 minutes before UUO, reduced the number of infiltrated Mø (P < 0.01). Conclusion. The results suggest that vasa recta, which express P-selectin, contribute to massive infiltration of Mø into the interstitium by functioning as specialized post-capillary venules.

AB - Background. Interstitial infiltration of macrophages (Mø) is one of the main causal factors for the tubulointerstitial injury. However, precise mechanisms of Mø infiltration into tubulointerstitium have not been fully explored. The purposes of this study were to assess the role of selectins in the acute infiltration of Mø in rats with unilateral ureteral obstruction (UUO) and to evaluate the role of vasa recta, that is, whether they facilitate massive influx of Mø into the interstitium by functioning as specialized vessels. Methods. To evaluate the role of selectins in Mø infiltration into tubulointerstitium, the expression of selectins and L-selectin ligands was examined by immunohistochemistry and immunoelectron microscopy. The functional role of P-selectin in vasa recta was studied by Stamper-Woodruff assay, in vivo p-Mø migration assay and in vivo blocking experiments with the monoclonal antibody (mAb) ARP2-4. Results. Selective expression of P-selectin was detected in vasa recta as early as one hour after UUO, and the expression increased thereafter for 96 hours. In contrast, endothelial expression of L-selectin ligands and E-selectin were not detectable. In the Stamper-Woodruff assay on kidney sections of rats with UUO, the adhesion of isolated rat peritoneal Mø (p-Mø) to vasa recta was significantly inhibited by the mAb ARP2-4 (P-selectin blocker; P < 0.01), but not by mAb ARE-5 (E-selectin blocker) or rLECIg (rat L-selectin chimeric protein). In the in vivo transfer experiments with fluorescein-labeled p-Mø into rats 48 hours after UUO, labeled p-Mø had accumulated around vasa recta at three minutes and had infiltrated predominantly into the outer medulla at 180 minutes. The number of labeled p-Mø was reduced when the rats were pretreated with ARP2-4 (P < 0.01). Finally, ARP2-4 (10 mg/kg), injected 15 minutes before UUO, reduced the number of infiltrated Mø (P < 0.01). Conclusion. The results suggest that vasa recta, which express P-selectin, contribute to massive infiltration of Mø into the interstitium by functioning as specialized post-capillary venules.

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