Paeonol attenuates TNBS-induced colitis by inhibiting NF-κB and STAT1 transactivation

Kazuhiro Ishiguro, Takafumi Ando, Osamu Maeda, Motofusa Hasegawa, Kenji Kadomatsu, Naoki Omiya, Yasumasa Niwa, Ramnik Xavier, Hidemi Goto

Research output: Contribution to journalArticle

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Abstract

Paeonol, a major phenolic component of Moutan Cortex, is known to have anti-inflammatory activity. However, the effect of Paeonol on colitis has not been evaluated and the molecular mechanism of its anti-inflammatory action remains unknown. The aim of this study was to determine if Paeonol enema attenuates trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. We also investigated the effects of Paeonol in colon cancer-derived CW-2 cells and T cell leukemia-derived Jurkat cells treated with tumor necrosis factor α (TNFα) and/or interferon γ (IFNγ), which play critical roles in TNBS-induced colitis. Paeonol enema attenuated TNBS-induced colitis judging by body weigh reduction, colon length and histological score. Myeloperoxidase activity and inducible nitric oxide synthase (iNOS) production in the colon were also reduced with Paeonol enema. In CW-2 cells, Paeonol inhibited iNOS protein and mRNA expression induced by costimulation of TNFα and IFNγ. Furthermore, Paeonol reduced TNFα-induced NF-κB transactivation and IFNγ-induced STAT1 transactivation in CW-2 cells and also in Jurkat cells. These findings suggest that Paeonol enema may be useful for the treatment of colitis.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalToxicology and Applied Pharmacology
Volume217
Issue number1
DOIs
Publication statusPublished - 15-11-2006
Externally publishedYes

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Trinitrobenzenes
Sulfonic Acids
Colitis
Transcriptional Activation
Enema
Interferons
Jurkat Cells
Tumor Necrosis Factor-alpha
Nitric Oxide Synthase Type II
Colon
Anti-Inflammatory Agents
T-Cell Leukemia
paeonol
T-cells
Colonic Neoplasms
Peroxidase

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Toxicology

Cite this

Ishiguro, Kazuhiro ; Ando, Takafumi ; Maeda, Osamu ; Hasegawa, Motofusa ; Kadomatsu, Kenji ; Omiya, Naoki ; Niwa, Yasumasa ; Xavier, Ramnik ; Goto, Hidemi. / Paeonol attenuates TNBS-induced colitis by inhibiting NF-κB and STAT1 transactivation. In: Toxicology and Applied Pharmacology. 2006 ; Vol. 217, No. 1. pp. 35-42.
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Ishiguro, K, Ando, T, Maeda, O, Hasegawa, M, Kadomatsu, K, Omiya, N, Niwa, Y, Xavier, R & Goto, H 2006, 'Paeonol attenuates TNBS-induced colitis by inhibiting NF-κB and STAT1 transactivation', Toxicology and Applied Pharmacology, vol. 217, no. 1, pp. 35-42. https://doi.org/10.1016/j.taap.2006.07.002

Paeonol attenuates TNBS-induced colitis by inhibiting NF-κB and STAT1 transactivation. / Ishiguro, Kazuhiro; Ando, Takafumi; Maeda, Osamu; Hasegawa, Motofusa; Kadomatsu, Kenji; Omiya, Naoki; Niwa, Yasumasa; Xavier, Ramnik; Goto, Hidemi.

In: Toxicology and Applied Pharmacology, Vol. 217, No. 1, 15.11.2006, p. 35-42.

Research output: Contribution to journalArticle

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T1 - Paeonol attenuates TNBS-induced colitis by inhibiting NF-κB and STAT1 transactivation

AU - Ishiguro, Kazuhiro

AU - Ando, Takafumi

AU - Maeda, Osamu

AU - Hasegawa, Motofusa

AU - Kadomatsu, Kenji

AU - Omiya, Naoki

AU - Niwa, Yasumasa

AU - Xavier, Ramnik

AU - Goto, Hidemi

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N2 - Paeonol, a major phenolic component of Moutan Cortex, is known to have anti-inflammatory activity. However, the effect of Paeonol on colitis has not been evaluated and the molecular mechanism of its anti-inflammatory action remains unknown. The aim of this study was to determine if Paeonol enema attenuates trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. We also investigated the effects of Paeonol in colon cancer-derived CW-2 cells and T cell leukemia-derived Jurkat cells treated with tumor necrosis factor α (TNFα) and/or interferon γ (IFNγ), which play critical roles in TNBS-induced colitis. Paeonol enema attenuated TNBS-induced colitis judging by body weigh reduction, colon length and histological score. Myeloperoxidase activity and inducible nitric oxide synthase (iNOS) production in the colon were also reduced with Paeonol enema. In CW-2 cells, Paeonol inhibited iNOS protein and mRNA expression induced by costimulation of TNFα and IFNγ. Furthermore, Paeonol reduced TNFα-induced NF-κB transactivation and IFNγ-induced STAT1 transactivation in CW-2 cells and also in Jurkat cells. These findings suggest that Paeonol enema may be useful for the treatment of colitis.

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