Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women

N. Masumoto, Takuma Fujii, M. Ishikawa, M. Mukai, M. Saito, T. Iwata, T. Fukuchi, K. Kubushiro, K. Tsukazaki, S. Nozawa

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A fluid-based Papanicolaou test has been established to improve sample collection and preparation. This study was the first largescale investigation in Japan to examine the feasibility of using fluid-based Papanicolaou specimens to detect human papillomavirus (HPV) using Hybrid Capture II and polymerase chain reaction (PCR). Three thousand patients who visited Keio University Hospital between October 2000 and February 2001 were enrolled in the study. The results of the fluid-based Papanicolaou tests corresponded well with those of conventional Papanicolaou smears (96.8% concordance). The sensitivities of cervical neoplasia detection using the fluid-based Papanicolaou test (73.9%) and Hybrid Capture II (76.3%, P=0.55) were not significantly different. Among the cervical intraepithelial neoplasia 3 and squamous cell carcinoma specimens, HPV 16 and HPV 52 were predominantly detected using the PCR method. Although some DNA samples extracted from the fluid-based specimens were degradaded, PCR and direct sequencing could be performed without difficulty even after 1 year of specimen storage. We conclude that fluid-based Papanicolaou specimens can be applied to investigate HPV infection.

Original languageEnglish
Pages (from-to)1883-1888
Number of pages6
JournalBritish Journal of Cancer
Volume88
Issue number12
DOIs
Publication statusPublished - 16-06-2003
Externally publishedYes

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Specimen Handling
Papanicolaou Test
Technology
Polymerase Chain Reaction
Cervical Intraepithelial Neoplasia
Papillomavirus Infections
Human papillomavirus 16
Squamous Cell Carcinoma
Japan
DNA
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Masumoto, N. ; Fujii, Takuma ; Ishikawa, M. ; Mukai, M. ; Saito, M. ; Iwata, T. ; Fukuchi, T. ; Kubushiro, K. ; Tsukazaki, K. ; Nozawa, S. / Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women. In: British Journal of Cancer. 2003 ; Vol. 88, No. 12. pp. 1883-1888.
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Masumoto, N, Fujii, T, Ishikawa, M, Mukai, M, Saito, M, Iwata, T, Fukuchi, T, Kubushiro, K, Tsukazaki, K & Nozawa, S 2003, 'Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women', British Journal of Cancer, vol. 88, no. 12, pp. 1883-1888. https://doi.org/10.1038/sj.bjc.6601023

Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women. / Masumoto, N.; Fujii, Takuma; Ishikawa, M.; Mukai, M.; Saito, M.; Iwata, T.; Fukuchi, T.; Kubushiro, K.; Tsukazaki, K.; Nozawa, S.

In: British Journal of Cancer, Vol. 88, No. 12, 16.06.2003, p. 1883-1888.

Research output: Contribution to journalArticle

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AU - Iwata, T.

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AU - Kubushiro, K.

AU - Tsukazaki, K.

AU - Nozawa, S.

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