TY - JOUR
T1 - Pattern of Expression of MicroRNA in Patients with Radiation-Induced Bladder Injury
AU - Nakamura, Ko
AU - Ohno, Takaya
AU - Inamoto, Teruo
AU - Takai, Tomoaki
AU - Uchimoto, Taizo
AU - Fukushima, Tatsuo
AU - Nishimura, Kazuki
AU - Yano, Yusuke
AU - Nishio, Kyosuke
AU - Kinoshita, Shoko
AU - Matsunaga, Tomohisa
AU - Nakamori, Keita
AU - Tsutsumi, Takeshi
AU - Tsujino, Takuya
AU - Uehara, Hirofumi
AU - Komura, Kazumasa
AU - Takahara, Kiyoshi
AU - Azuma, Haruhito
N1 - Publisher Copyright:
© 2024 S. Karger AG. All rights reserved.
PY - 2024/7/1
Y1 - 2024/7/1
N2 - Introduction: Bladder cancer (BC) is sensitive to radiation treatment and a subset of patients experience radiationinduced injuries including shrinkage of bladder due to bladder fibrosis. Methods: This study is a retrospective cohort study. Three Japanese BC patients were randomly selected. Using a microRNA (miRNA) array, comparing their samples with or without radiation-induced injuries, we have checked the clustering of miRNA expression. Results: Hsa-miR-130a, hsa-miR-200c, hsa-miR-141, and hsa-miR-96 were found to be highly expressed (>50 times) in patients with fibrotic bladder shrinkage (FBS) compared to those with intact bladder (IB) function. In patients with FBS, hsa-miR-6835, hsa-miR-4675, hsa-miR- 371a, and hsa-miR-6885 were detected to have lesser than half expression to IB patients. We have analyzed the significance of these genes in relation to overall survival of 409 BC patients retrieved from the Cancer Genome Atlas data set. All available cutoff values between the lower and upper quartiles of expression are used for the selected genes, and false discovery rate using the Benjamini-Hochberg method is computed to correct for multiple hypothesis testing. We have run combined survival analysis of the mean expression of these four miRNAs highly expressed in FBS patients. 175 patients with high expression had a longer median survival of 98.47 months than 23.73 months in 233 patients with low expression (hazard ratio [HR]: 0.53; 0.39-0.72, log-rank p value: 7.3e-0.5). Combination analysis of all 8 genes including hsa-miR-6835, hsa-miR-4675, hsa-miR-371a, and hsa-miR-6885 showed the same HR for OS. Target scanning for these miRNAs matched specific cytokines known as an early biomarker to develop radiationinduced fibrosis. Conclusions: BC patients with fibrotic radiation injury have specific miRNA expression profile targeting profibrotic cytokines and these miRNAs possibly render to favorable survival. 2023 S. Karger AG, Basel.
AB - Introduction: Bladder cancer (BC) is sensitive to radiation treatment and a subset of patients experience radiationinduced injuries including shrinkage of bladder due to bladder fibrosis. Methods: This study is a retrospective cohort study. Three Japanese BC patients were randomly selected. Using a microRNA (miRNA) array, comparing their samples with or without radiation-induced injuries, we have checked the clustering of miRNA expression. Results: Hsa-miR-130a, hsa-miR-200c, hsa-miR-141, and hsa-miR-96 were found to be highly expressed (>50 times) in patients with fibrotic bladder shrinkage (FBS) compared to those with intact bladder (IB) function. In patients with FBS, hsa-miR-6835, hsa-miR-4675, hsa-miR- 371a, and hsa-miR-6885 were detected to have lesser than half expression to IB patients. We have analyzed the significance of these genes in relation to overall survival of 409 BC patients retrieved from the Cancer Genome Atlas data set. All available cutoff values between the lower and upper quartiles of expression are used for the selected genes, and false discovery rate using the Benjamini-Hochberg method is computed to correct for multiple hypothesis testing. We have run combined survival analysis of the mean expression of these four miRNAs highly expressed in FBS patients. 175 patients with high expression had a longer median survival of 98.47 months than 23.73 months in 233 patients with low expression (hazard ratio [HR]: 0.53; 0.39-0.72, log-rank p value: 7.3e-0.5). Combination analysis of all 8 genes including hsa-miR-6835, hsa-miR-4675, hsa-miR-371a, and hsa-miR-6885 showed the same HR for OS. Target scanning for these miRNAs matched specific cytokines known as an early biomarker to develop radiationinduced fibrosis. Conclusions: BC patients with fibrotic radiation injury have specific miRNA expression profile targeting profibrotic cytokines and these miRNAs possibly render to favorable survival. 2023 S. Karger AG, Basel.
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U2 - 10.1159/000535993
DO - 10.1159/000535993
M3 - Article
C2 - 38160665
AN - SCOPUS:85194776844
SN - 0030-2414
VL - 102
SP - 585
EP - 592
JO - Oncology (Switzerland)
JF - Oncology (Switzerland)
IS - 7
ER -