TY - JOUR
T1 - PCR detection of metallo-β-lactamase gene (bla(IMP)) in gram-negative rods resistant to broad-spectrum β-lactams
AU - Senda, Kazuyoshi
AU - Arakawa, Yoshichika
AU - Ichiyama, Satoshi
AU - Nakashima, Kazumitsu
AU - Ito, Hideo
AU - Ohsuka, Shinji
AU - Shimokata, Kaoru
AU - Kato, Nobuo
AU - Ohta, Michio
PY - 1996/12
Y1 - 1996/12
N2 - We applied PCR to the rapid detection of the metallo-β-lactamase gene, bla(IMP) in clinically isolated gram-negative rods. A total of 54 high-level ceftazidime-resistant strains (MICs, > 128 μg/ml) were subjected to PCR analyses with the bla(IMP)-specific primers, since the bla(IMP)-bearing clinical isolates tested in our previous study always demonstrated high- level resistance to ceftazidime. Twenty-two bla(IMP)-positive strains including 9 Pseudomonas aeruginosa, 9 Serratia marcescens, 2 Alcaligenes xylosoxidans, 1 Pseudomonas putida, and 1 Klebsiella pneumoniae strains were newly identified from 18 different hospitals in Japan. These strains were mostly isolated from urine samples and showed high-level resistance to almost every cephem, while their levels of resistance to carbapenems were diverse. The PCR analyses with novel integrase gene-specific (intI3) and acc(6')-Ib gene-specific primers suggested that the integron structure found in a large plasmid harbored by S. marcescens AK9373 was also well conserved among bla(IMP)-positive strains. These results imply that the bla(IMP) gene cassettes have been dispersing into various gram-negative rods with the help of the newly identified integron element. Thus, the PCR-aided rapid detection will be helpful for the early recognition of emerging bla(IMP)-positive clinical isolates which demonstrate consistent resistance to β-lactams.
AB - We applied PCR to the rapid detection of the metallo-β-lactamase gene, bla(IMP) in clinically isolated gram-negative rods. A total of 54 high-level ceftazidime-resistant strains (MICs, > 128 μg/ml) were subjected to PCR analyses with the bla(IMP)-specific primers, since the bla(IMP)-bearing clinical isolates tested in our previous study always demonstrated high- level resistance to ceftazidime. Twenty-two bla(IMP)-positive strains including 9 Pseudomonas aeruginosa, 9 Serratia marcescens, 2 Alcaligenes xylosoxidans, 1 Pseudomonas putida, and 1 Klebsiella pneumoniae strains were newly identified from 18 different hospitals in Japan. These strains were mostly isolated from urine samples and showed high-level resistance to almost every cephem, while their levels of resistance to carbapenems were diverse. The PCR analyses with novel integrase gene-specific (intI3) and acc(6')-Ib gene-specific primers suggested that the integron structure found in a large plasmid harbored by S. marcescens AK9373 was also well conserved among bla(IMP)-positive strains. These results imply that the bla(IMP) gene cassettes have been dispersing into various gram-negative rods with the help of the newly identified integron element. Thus, the PCR-aided rapid detection will be helpful for the early recognition of emerging bla(IMP)-positive clinical isolates which demonstrate consistent resistance to β-lactams.
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U2 - 10.1128/jcm.34.12.2909-2913.1996
DO - 10.1128/jcm.34.12.2909-2913.1996
M3 - Article
C2 - 8940421
AN - SCOPUS:0029860992
SN - 0095-1137
VL - 34
SP - 2909
EP - 2913
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 12
ER -