PCR Typing of Genetic Determinants for Metallo-β-Lactamases and Integrases Carried by Gram-Negative Bacteria Isolated in Japan, with Focus on the Class 3 Integron

Naohiro Shibata, Yohei Doi, Kunikazu Yamane, Tetsuya Yagi, Hiroshi Kurokawa, Keigo Shibayama, Haru Kato, Kumiko Kai, Yoshichika Arakawa

Research output: Contribution to journalArticle

239 Citations (Scopus)

Abstract

From January 2001 to December 2002, 587 strains of gram-negative bacterial isolates demonstrating resistance to ceftazidime and a combination of sulbactam and cefoperazone were subjected to a disk diffusion screening test using sodium mercaptoacetic acid; 431 strains (73.4%) appeared to produce metallo-β-lactamase (MBL). Of these 431 strains, 357 were found by PCR to carry genes for IMP-1 type MBL (blaIMP-1), while only 7 and 67 strains carried the IMP-2 gene (blaIMP-2) and the VIM-2 gene (blaVIM-2), respectively. Neither VIM-1 nor SPM-1 type MBL genes were found among the strains tested. Of 431 strains, 427 carried the intI1 gene, and 4 strains carrying both the intI1 and intI3 genes were reidentified as Pseudomonas putida harboring blaIMP-1. Of these four P. putida strains, three strains and one strain, respectively, were separately isolated from two hospitals located in the same prefecture, and the three strains showed very similar pulsed-field gel electrophoresis patterns. Of 357 bla IMP-1 carriers, 116, 53, 51, 47, and 30 strains were identified as Pseudomonas aeruginosa, Alcaligenes xylosoxidans, P. putida/fluorescens, Serratia marcescens, and Acinetobacter baumannii, respectively. Four strains carrying blaIMP-2 were reidentified as P. putida. Sixty-three P. aeruginosa strains and four P. putida strains carried blaVIM-2. Of 427 intI1-positive strains, 180, 53, 51, 47, and 35 were identified as P. aeruginosa, A. xylosoxidans, P. putida/fluorescens, S. marcescens, and A. baumannii, respectively. In the present study, it was confirmed that strains carrying blaIMP-1 with a class 1 integron are the most prevalent type in Japan, although several intI3 carriers have also been identified sporadically in this country.

Original languageEnglish
Pages (from-to)5407-5413
Number of pages7
JournalJournal of clinical microbiology
Volume41
Issue number12
DOIs
Publication statusPublished - 01-12-2003

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Integrons
Integrases
Pseudomonas putida
Gram-Negative Bacteria
Japan
Polymerase Chain Reaction
Inosine Monophosphate
Genes
Pseudomonas aeruginosa
Acinetobacter baumannii
Pseudomonas fluorescens
Serratia marcescens
Pseudomonas alcaligenes
Cefoperazone
Sulbactam
Ceftazidime
Pulsed Field Gel Electrophoresis
Sodium

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Shibata, Naohiro ; Doi, Yohei ; Yamane, Kunikazu ; Yagi, Tetsuya ; Kurokawa, Hiroshi ; Shibayama, Keigo ; Kato, Haru ; Kai, Kumiko ; Arakawa, Yoshichika. / PCR Typing of Genetic Determinants for Metallo-β-Lactamases and Integrases Carried by Gram-Negative Bacteria Isolated in Japan, with Focus on the Class 3 Integron. In: Journal of clinical microbiology. 2003 ; Vol. 41, No. 12. pp. 5407-5413.
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PCR Typing of Genetic Determinants for Metallo-β-Lactamases and Integrases Carried by Gram-Negative Bacteria Isolated in Japan, with Focus on the Class 3 Integron. / Shibata, Naohiro; Doi, Yohei; Yamane, Kunikazu; Yagi, Tetsuya; Kurokawa, Hiroshi; Shibayama, Keigo; Kato, Haru; Kai, Kumiko; Arakawa, Yoshichika.

In: Journal of clinical microbiology, Vol. 41, No. 12, 01.12.2003, p. 5407-5413.

Research output: Contribution to journalArticle

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AU - Shibata, Naohiro

AU - Doi, Yohei

AU - Yamane, Kunikazu

AU - Yagi, Tetsuya

AU - Kurokawa, Hiroshi

AU - Shibayama, Keigo

AU - Kato, Haru

AU - Kai, Kumiko

AU - Arakawa, Yoshichika

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