PD-L1 upregulation by lytic induction of Epstein-Barr Virus

Yusuke Yanagi, Yuya Hara, Seiyo Mabuchi, Takahiro Watanabe, Yoshitaka Sato, Hiroshi Kimura, Takayuki Murata

Research output: Contribution to journalArticlepeer-review

Abstract

Epstein-Barr virus (EBV) is an etiologic agent of infectious mononucleosis and several malignancies. Here, we found that reactivation of EBV resulted in increased programmed cell death-ligand 1 (PD-L1) expression in a cell type-dependent manner. Lytic induction in EBV-positive Akata, AGS, MutuI, and Jijoye cell lines increased PD-L1 levels, but cells such as EBV-negative Akata, MutuIII, and P3HR1 did not have increased PD-L1. EBV in the P3HR1 cell line has a deletion in the EBNA2 gene, while EBV in its parental cell line, Jijoye, has the complete EBNA2 gene. PD-L1 expression by lytic induction was reduced when EBNA2 was knocked down. In addition, pharmacological inhibition indicated involvement of nuclear factor kappa B, mitogen-activated protein kinase, and AKT signaling. These results suggest that EBV likely evades immunity by inducing PD-L1 upon reactivation, through the increased expression of EBNA2 and activation of signaling pathways.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalVirology
Volume568
DOIs
Publication statusPublished - 03-2022

All Science Journal Classification (ASJC) codes

  • Virology

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