TY - JOUR
T1 - Pea3 determines the isthmus region at the downstream of Fgf8-Ras-ERK signaling pathway
AU - Harada, Hidekiyo
AU - Omi, Minoru
AU - Sato, Tatsuya
AU - Nakamura, Harukazu
N1 - Funding Information:
This study was supported partly by a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan (17023003); and by a grant from the Japanese Society for the Promotion of Science (22570199 and 06J05334). H.H. was a Research Fellowship for Young Scientists of Japanese Society for the Promotion of Sciences.
Publisher Copyright:
© 2015 Japanese Society of Developmental Biologists.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - It has been shown that strong Fgf8 signal activates Ras-ERK signaling pathway to determine metencephalon, which consists of rhombomere 1 (r1), where the cerebellum differentiates, and isthmus (r0). The present study was undertaken to check if Ets type transcription factor Pea3 functions downstream of Ras-ERK signaling to determine metencephalon. Pea3 misexpression resulted in repression of Otx2 expression in the mesencephalon, induction of Gbx2 and Fgf8 expression in the mesencephalon, and differentiation of the trochlear neurons in the posterior mesencephalon. Fate change of the tectum to the cerebellum did not occur. Repression of Pea3 function by misexpressing the chimeric molecule of Engrailed repressor domain EH1 and Pea3 (eh1-Pea3) resulted in induction of Otx2 expression in the metencephalon, repression of Gbx2 and Fgf8 expression in the metencephalon, and differentiation of the oculomotor neurons in the isthmus. It was concluded that Pea3 plays a pivotal role in determination of the isthmus (r0) property downstream of Fgf8-Ras-ERK signaling.
AB - It has been shown that strong Fgf8 signal activates Ras-ERK signaling pathway to determine metencephalon, which consists of rhombomere 1 (r1), where the cerebellum differentiates, and isthmus (r0). The present study was undertaken to check if Ets type transcription factor Pea3 functions downstream of Ras-ERK signaling to determine metencephalon. Pea3 misexpression resulted in repression of Otx2 expression in the mesencephalon, induction of Gbx2 and Fgf8 expression in the mesencephalon, and differentiation of the trochlear neurons in the posterior mesencephalon. Fate change of the tectum to the cerebellum did not occur. Repression of Pea3 function by misexpressing the chimeric molecule of Engrailed repressor domain EH1 and Pea3 (eh1-Pea3) resulted in induction of Otx2 expression in the metencephalon, repression of Gbx2 and Fgf8 expression in the metencephalon, and differentiation of the oculomotor neurons in the isthmus. It was concluded that Pea3 plays a pivotal role in determination of the isthmus (r0) property downstream of Fgf8-Ras-ERK signaling.
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U2 - 10.1111/dgd.12254
DO - 10.1111/dgd.12254
M3 - Article
C2 - 26691276
AN - SCOPUS:84955188844
SN - 0012-1592
VL - 57
SP - 657
EP - 666
JO - Development Growth and Differentiation
JF - Development Growth and Differentiation
IS - 9
ER -