Periodontal Regeneration by Allogeneic Transplantation of Adipose Tissue Derived Multi-Lineage Progenitor Stem Cells in vivo

Venkata Suresh Venkataiah, Keisuke Handa, Mary M. Njuguna, Tatsuya Hasegawa, Kentaro Maruyama, Eiji Nemoto, Satoru Yamada, Shunji Sugawara, Lu Lu, Masahide Takedachi, Shinya Murakami, Hanayuki Okura, Akifumi Matsuyama, Masahiro Saito

Research output: Contribution to journalArticle

Abstract

The ultimate goal of periodontal disease treatment is the reorganization of functional tissue that can regenerate lost periodontal tissue. Regeneration of periodontal tissues is clinically possible by using autogenic transplantation of MSCs. However, autologous MSC transplantation is limited depending on age, systemic disease and tissue quality, thus precluding their clinical application. Therefore, we evaluated the efficacy of allogeneic transplantation of adipose-derived multi-lineage progenitor cells (ADMPC) in a micro-mini pig periodontal defect model. ADMPC were isolated from the greater omentum of micro-mini pigs, and flow cytometry analysis confirmed that the ADMPC expressed MSC markers, including CD44 and CD73. ADMPC exhibited osteogenic, adipogenic and periodontal ligament differentiation capacities in differentiation medium. ADMPC showed high expression of the immune suppressive factors GBP4 and IL1-RA upon treatment with a cytokine cocktail containing interferon-γ, tumor necrosis factor-α and interleukin-6. Allogeneic transplantation of ADMPC in a micro-mini pig periodontal defect model showed significant bone regeneration ability based on bone-morphometric analysis. Moreover, the regeneration ability of ADMPC by allogeneic transplantation was comparable to those of autologous transplantation by histological analysis. These results indicate that ADMPC have immune-modulation capability that can induce periodontal tissue regeneration by allogeneic transplantation.

Original languageEnglish
Article number921
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 01-12-2019

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Homologous Transplantation
Adipose Tissue
Regeneration
Stem Cells
Swine
Autologous Transplantation
Periodontal Ligament
Omentum
Bone Regeneration
Cell Transplantation
Immunologic Factors
Periodontal Diseases
Interferons
Interleukin-6
Flow Cytometry
Tumor Necrosis Factor-alpha
Transplantation
Cytokines
Bone and Bones
Therapeutics

All Science Journal Classification (ASJC) codes

  • General

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Venkataiah, V. S., Handa, K., Njuguna, M. M., Hasegawa, T., Maruyama, K., Nemoto, E., ... Saito, M. (2019). Periodontal Regeneration by Allogeneic Transplantation of Adipose Tissue Derived Multi-Lineage Progenitor Stem Cells in vivo. Scientific Reports, 9(1), [921]. https://doi.org/10.1038/s41598-018-37528-0
Venkataiah, Venkata Suresh ; Handa, Keisuke ; Njuguna, Mary M. ; Hasegawa, Tatsuya ; Maruyama, Kentaro ; Nemoto, Eiji ; Yamada, Satoru ; Sugawara, Shunji ; Lu, Lu ; Takedachi, Masahide ; Murakami, Shinya ; Okura, Hanayuki ; Matsuyama, Akifumi ; Saito, Masahiro. / Periodontal Regeneration by Allogeneic Transplantation of Adipose Tissue Derived Multi-Lineage Progenitor Stem Cells in vivo. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "The ultimate goal of periodontal disease treatment is the reorganization of functional tissue that can regenerate lost periodontal tissue. Regeneration of periodontal tissues is clinically possible by using autogenic transplantation of MSCs. However, autologous MSC transplantation is limited depending on age, systemic disease and tissue quality, thus precluding their clinical application. Therefore, we evaluated the efficacy of allogeneic transplantation of adipose-derived multi-lineage progenitor cells (ADMPC) in a micro-mini pig periodontal defect model. ADMPC were isolated from the greater omentum of micro-mini pigs, and flow cytometry analysis confirmed that the ADMPC expressed MSC markers, including CD44 and CD73. ADMPC exhibited osteogenic, adipogenic and periodontal ligament differentiation capacities in differentiation medium. ADMPC showed high expression of the immune suppressive factors GBP4 and IL1-RA upon treatment with a cytokine cocktail containing interferon-γ, tumor necrosis factor-α and interleukin-6. Allogeneic transplantation of ADMPC in a micro-mini pig periodontal defect model showed significant bone regeneration ability based on bone-morphometric analysis. Moreover, the regeneration ability of ADMPC by allogeneic transplantation was comparable to those of autologous transplantation by histological analysis. These results indicate that ADMPC have immune-modulation capability that can induce periodontal tissue regeneration by allogeneic transplantation.",
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Venkataiah, VS, Handa, K, Njuguna, MM, Hasegawa, T, Maruyama, K, Nemoto, E, Yamada, S, Sugawara, S, Lu, L, Takedachi, M, Murakami, S, Okura, H, Matsuyama, A & Saito, M 2019, 'Periodontal Regeneration by Allogeneic Transplantation of Adipose Tissue Derived Multi-Lineage Progenitor Stem Cells in vivo', Scientific Reports, vol. 9, no. 1, 921. https://doi.org/10.1038/s41598-018-37528-0

Periodontal Regeneration by Allogeneic Transplantation of Adipose Tissue Derived Multi-Lineage Progenitor Stem Cells in vivo. / Venkataiah, Venkata Suresh; Handa, Keisuke; Njuguna, Mary M.; Hasegawa, Tatsuya; Maruyama, Kentaro; Nemoto, Eiji; Yamada, Satoru; Sugawara, Shunji; Lu, Lu; Takedachi, Masahide; Murakami, Shinya; Okura, Hanayuki; Matsuyama, Akifumi; Saito, Masahiro.

In: Scientific Reports, Vol. 9, No. 1, 921, 01.12.2019.

Research output: Contribution to journalArticle

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T1 - Periodontal Regeneration by Allogeneic Transplantation of Adipose Tissue Derived Multi-Lineage Progenitor Stem Cells in vivo

AU - Venkataiah, Venkata Suresh

AU - Handa, Keisuke

AU - Njuguna, Mary M.

AU - Hasegawa, Tatsuya

AU - Maruyama, Kentaro

AU - Nemoto, Eiji

AU - Yamada, Satoru

AU - Sugawara, Shunji

AU - Lu, Lu

AU - Takedachi, Masahide

AU - Murakami, Shinya

AU - Okura, Hanayuki

AU - Matsuyama, Akifumi

AU - Saito, Masahiro

PY - 2019/12/1

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N2 - The ultimate goal of periodontal disease treatment is the reorganization of functional tissue that can regenerate lost periodontal tissue. Regeneration of periodontal tissues is clinically possible by using autogenic transplantation of MSCs. However, autologous MSC transplantation is limited depending on age, systemic disease and tissue quality, thus precluding their clinical application. Therefore, we evaluated the efficacy of allogeneic transplantation of adipose-derived multi-lineage progenitor cells (ADMPC) in a micro-mini pig periodontal defect model. ADMPC were isolated from the greater omentum of micro-mini pigs, and flow cytometry analysis confirmed that the ADMPC expressed MSC markers, including CD44 and CD73. ADMPC exhibited osteogenic, adipogenic and periodontal ligament differentiation capacities in differentiation medium. ADMPC showed high expression of the immune suppressive factors GBP4 and IL1-RA upon treatment with a cytokine cocktail containing interferon-γ, tumor necrosis factor-α and interleukin-6. Allogeneic transplantation of ADMPC in a micro-mini pig periodontal defect model showed significant bone regeneration ability based on bone-morphometric analysis. Moreover, the regeneration ability of ADMPC by allogeneic transplantation was comparable to those of autologous transplantation by histological analysis. These results indicate that ADMPC have immune-modulation capability that can induce periodontal tissue regeneration by allogeneic transplantation.

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