Peripheral injection of lipopolysaccharide enhances expression of inflammatory cytokines in murine locus coeruleus

Possible role of increased norepinephrine turnover

Yoko S. Kaneko, Keiji Mori, Akira Nakashima, Makoto Sawada, Ikuko Nagatsu, Akira Ota

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cytokines and catecholamines are known to constitute a significant portion of the regulatory neuroimmune networks involved in maintaining homeostasis in the central nervous system (CNS). Although we have already reported an increase in norepinephrine (NE) turnover within the locus coeruleus (LC) at 2 and 4 h after the intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), the implication of this increase remains unclear. In view of evidence that norepinephrine (NE) acts in an anti-inflammatory manner by way of negatively regulating pro-inflammatory cytokine expression, we examined the inflammatory cytokine expression levels in the LC of C3H/HeN mice (male, 8 weeks old) after an i.p. LPS injection. The mRNA expression levels of the genes encoding IL-1β and TNF-α within the LC increased during the first 2 h, and showed two peaks, the first at 4 h and the second lesser one at 15 h after the LPS injection. Microglia, which are one of the major cell types that produce pro-inflammatory cytokines in the CNS, were isolated from mouse neonate brains in order to clarify more precisely the relationship between the changes in NE content and the up-regulation of inflammatory cytokines in the LC. Simultaneous incubation of microglia with LPS and NE enhanced the expression of IL-1β at both mRNA and protein levels, but reduced the mRNA and protein levels of TNF-α. These data support the hypothesis that NE negatively regulates the expression of pro-inflammatory cytokine expression, at least in the case of TNF-α, which action could contribute to the observed anti-inflammatory properties of NE. This report, based on the results of both in vivo and in vitro experiments, is the first to suggest a relationship between NE content and cytokine expression levels in the CNS.

Original languageEnglish
Pages (from-to)393-404
Number of pages12
JournalJournal of Neurochemistry
Volume94
Issue number2
DOIs
Publication statusPublished - 01-07-2005

Fingerprint

Locus Coeruleus
Lipopolysaccharides
Norepinephrine
Cytokines
Injections
Neurology
Central Nervous System
Microglia
Intraperitoneal Injections
Interleukin-1
Messenger RNA
Anti-Inflammatory Agents
Gene encoding
Inbred C3H Mouse
Catecholamines
Brain
Proteins
Homeostasis
Up-Regulation
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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title = "Peripheral injection of lipopolysaccharide enhances expression of inflammatory cytokines in murine locus coeruleus: Possible role of increased norepinephrine turnover",
abstract = "Cytokines and catecholamines are known to constitute a significant portion of the regulatory neuroimmune networks involved in maintaining homeostasis in the central nervous system (CNS). Although we have already reported an increase in norepinephrine (NE) turnover within the locus coeruleus (LC) at 2 and 4 h after the intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), the implication of this increase remains unclear. In view of evidence that norepinephrine (NE) acts in an anti-inflammatory manner by way of negatively regulating pro-inflammatory cytokine expression, we examined the inflammatory cytokine expression levels in the LC of C3H/HeN mice (male, 8 weeks old) after an i.p. LPS injection. The mRNA expression levels of the genes encoding IL-1β and TNF-α within the LC increased during the first 2 h, and showed two peaks, the first at 4 h and the second lesser one at 15 h after the LPS injection. Microglia, which are one of the major cell types that produce pro-inflammatory cytokines in the CNS, were isolated from mouse neonate brains in order to clarify more precisely the relationship between the changes in NE content and the up-regulation of inflammatory cytokines in the LC. Simultaneous incubation of microglia with LPS and NE enhanced the expression of IL-1β at both mRNA and protein levels, but reduced the mRNA and protein levels of TNF-α. These data support the hypothesis that NE negatively regulates the expression of pro-inflammatory cytokine expression, at least in the case of TNF-α, which action could contribute to the observed anti-inflammatory properties of NE. This report, based on the results of both in vivo and in vitro experiments, is the first to suggest a relationship between NE content and cytokine expression levels in the CNS.",
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Peripheral injection of lipopolysaccharide enhances expression of inflammatory cytokines in murine locus coeruleus : Possible role of increased norepinephrine turnover. / Kaneko, Yoko S.; Mori, Keiji; Nakashima, Akira; Sawada, Makoto; Nagatsu, Ikuko; Ota, Akira.

In: Journal of Neurochemistry, Vol. 94, No. 2, 01.07.2005, p. 393-404.

Research output: Contribution to journalArticle

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