Peripheral lipopolysaccharide administration affects the olfactory dopamine system in mice

Akira Ota, Keiji Mori, Yoko S. Kaneko, Akira Nakashima, Ikuko Nagatsu, Toshiharu Nagatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

Peripheral administration of lipopolysaccharide (LPS) in an amount that produces acute stress has been found to affect the catecholamine systems in the brain. Acute peripheral LPS administration activated norepinephrine (NE) metabolism in the locus ceruleus (LC). Approximately 40% of murine LC neurons project to the olfactory bulb (OB) and the anterior olfactory nucleus (AON). Thus, we investigated the effects of a single intra-peritoneal (i.p.) LPS injection on catecholamine biosynthesis in the OB and AON in 8-week-old C3H/HeN male mice. In the AON, the content of dopamine (DA), but not that of NE, was highly increased 6 h after LPS injection. In the OB, the contents of DA and NE did not change; but within 2 h after a single i.p. LPS injection, the mRNA levels of IκB, TNF-α, and TNF-α receptor type 1 were significantly enhanced. Almost all TNF-α-immunoreactive cells in the OB of the LPS-injected mice were located in the granule cell layer, and unexpectedly, they were not microglia but astroglia. The number of TUNEL-positive cells identified exclusively in the granule cell layer was significantly increased at 24 h after LPS injection. Therefore, our data suggest that astroglia activated by peripherally injected LPS may release TNF-α, which may trigger apoptosis in the granule cell layer in the OB. The increase in DA content in the AON and the production of TNF-α and apoptotic cells in the OB by acute peripheral LPS administration are not likely to be related.

Original languageEnglish
Title of host publicationStress, Neurotransmitters, and Hormones Neuroendocrine and Genetic Mechanisms
PublisherBlackwell Publishing Inc.
Pages127-135
Number of pages9
ISBN (Print)9781573316927
DOIs
Publication statusPublished - 01-01-2008

Publication series

NameAnnals of the New York Academy of Sciences
Volume1148
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Lipopolysaccharides
Dopamine
Olfactory Bulb
Cells
Norepinephrine
Injections
Locus Coeruleus
Astrocytes
Catecholamines
Mouse
Tumor Necrosis Factor Receptors
Biosynthesis
In Situ Nick-End Labeling
Microglia
Metabolism
Neurons
Nucleus
Brain
Apoptosis
Layer

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Ota, A., Mori, K., Kaneko, Y. S., Nakashima, A., Nagatsu, I., & Nagatsu, T. (2008). Peripheral lipopolysaccharide administration affects the olfactory dopamine system in mice. In Stress, Neurotransmitters, and Hormones Neuroendocrine and Genetic Mechanisms (pp. 127-135). (Annals of the New York Academy of Sciences; Vol. 1148). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1410.071
Ota, Akira ; Mori, Keiji ; Kaneko, Yoko S. ; Nakashima, Akira ; Nagatsu, Ikuko ; Nagatsu, Toshiharu. / Peripheral lipopolysaccharide administration affects the olfactory dopamine system in mice. Stress, Neurotransmitters, and Hormones Neuroendocrine and Genetic Mechanisms. Blackwell Publishing Inc., 2008. pp. 127-135 (Annals of the New York Academy of Sciences).
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abstract = "Peripheral administration of lipopolysaccharide (LPS) in an amount that produces acute stress has been found to affect the catecholamine systems in the brain. Acute peripheral LPS administration activated norepinephrine (NE) metabolism in the locus ceruleus (LC). Approximately 40{\%} of murine LC neurons project to the olfactory bulb (OB) and the anterior olfactory nucleus (AON). Thus, we investigated the effects of a single intra-peritoneal (i.p.) LPS injection on catecholamine biosynthesis in the OB and AON in 8-week-old C3H/HeN male mice. In the AON, the content of dopamine (DA), but not that of NE, was highly increased 6 h after LPS injection. In the OB, the contents of DA and NE did not change; but within 2 h after a single i.p. LPS injection, the mRNA levels of IκB, TNF-α, and TNF-α receptor type 1 were significantly enhanced. Almost all TNF-α-immunoreactive cells in the OB of the LPS-injected mice were located in the granule cell layer, and unexpectedly, they were not microglia but astroglia. The number of TUNEL-positive cells identified exclusively in the granule cell layer was significantly increased at 24 h after LPS injection. Therefore, our data suggest that astroglia activated by peripherally injected LPS may release TNF-α, which may trigger apoptosis in the granule cell layer in the OB. The increase in DA content in the AON and the production of TNF-α and apoptotic cells in the OB by acute peripheral LPS administration are not likely to be related.",
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Ota, A, Mori, K, Kaneko, YS, Nakashima, A, Nagatsu, I & Nagatsu, T 2008, Peripheral lipopolysaccharide administration affects the olfactory dopamine system in mice. in Stress, Neurotransmitters, and Hormones Neuroendocrine and Genetic Mechanisms. Annals of the New York Academy of Sciences, vol. 1148, Blackwell Publishing Inc., pp. 127-135. https://doi.org/10.1196/annals.1410.071

Peripheral lipopolysaccharide administration affects the olfactory dopamine system in mice. / Ota, Akira; Mori, Keiji; Kaneko, Yoko S.; Nakashima, Akira; Nagatsu, Ikuko; Nagatsu, Toshiharu.

Stress, Neurotransmitters, and Hormones Neuroendocrine and Genetic Mechanisms. Blackwell Publishing Inc., 2008. p. 127-135 (Annals of the New York Academy of Sciences; Vol. 1148).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Ota, Akira

AU - Mori, Keiji

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AU - Nakashima, Akira

AU - Nagatsu, Ikuko

AU - Nagatsu, Toshiharu

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N2 - Peripheral administration of lipopolysaccharide (LPS) in an amount that produces acute stress has been found to affect the catecholamine systems in the brain. Acute peripheral LPS administration activated norepinephrine (NE) metabolism in the locus ceruleus (LC). Approximately 40% of murine LC neurons project to the olfactory bulb (OB) and the anterior olfactory nucleus (AON). Thus, we investigated the effects of a single intra-peritoneal (i.p.) LPS injection on catecholamine biosynthesis in the OB and AON in 8-week-old C3H/HeN male mice. In the AON, the content of dopamine (DA), but not that of NE, was highly increased 6 h after LPS injection. In the OB, the contents of DA and NE did not change; but within 2 h after a single i.p. LPS injection, the mRNA levels of IκB, TNF-α, and TNF-α receptor type 1 were significantly enhanced. Almost all TNF-α-immunoreactive cells in the OB of the LPS-injected mice were located in the granule cell layer, and unexpectedly, they were not microglia but astroglia. The number of TUNEL-positive cells identified exclusively in the granule cell layer was significantly increased at 24 h after LPS injection. Therefore, our data suggest that astroglia activated by peripherally injected LPS may release TNF-α, which may trigger apoptosis in the granule cell layer in the OB. The increase in DA content in the AON and the production of TNF-α and apoptotic cells in the OB by acute peripheral LPS administration are not likely to be related.

AB - Peripheral administration of lipopolysaccharide (LPS) in an amount that produces acute stress has been found to affect the catecholamine systems in the brain. Acute peripheral LPS administration activated norepinephrine (NE) metabolism in the locus ceruleus (LC). Approximately 40% of murine LC neurons project to the olfactory bulb (OB) and the anterior olfactory nucleus (AON). Thus, we investigated the effects of a single intra-peritoneal (i.p.) LPS injection on catecholamine biosynthesis in the OB and AON in 8-week-old C3H/HeN male mice. In the AON, the content of dopamine (DA), but not that of NE, was highly increased 6 h after LPS injection. In the OB, the contents of DA and NE did not change; but within 2 h after a single i.p. LPS injection, the mRNA levels of IκB, TNF-α, and TNF-α receptor type 1 were significantly enhanced. Almost all TNF-α-immunoreactive cells in the OB of the LPS-injected mice were located in the granule cell layer, and unexpectedly, they were not microglia but astroglia. The number of TUNEL-positive cells identified exclusively in the granule cell layer was significantly increased at 24 h after LPS injection. Therefore, our data suggest that astroglia activated by peripherally injected LPS may release TNF-α, which may trigger apoptosis in the granule cell layer in the OB. The increase in DA content in the AON and the production of TNF-α and apoptotic cells in the OB by acute peripheral LPS administration are not likely to be related.

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Ota A, Mori K, Kaneko YS, Nakashima A, Nagatsu I, Nagatsu T. Peripheral lipopolysaccharide administration affects the olfactory dopamine system in mice. In Stress, Neurotransmitters, and Hormones Neuroendocrine and Genetic Mechanisms. Blackwell Publishing Inc. 2008. p. 127-135. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1410.071