Peripheral lipopolysaccharide induces apoptosis in the murine olfactory bulb

Keiji Mori, Yoko S. Kaneko, Akira Nakashima, Ikuko Nagatsu, Hisahide Takahashi, Akira Ota

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The olfactory bulb (OB) is one of the few structures in the adult mammalian CNS that contains a continuous supply of newly generated neurons in the subventricular zone. Therefore, the balance between the supply of new cells and apoptosis in the OB might determine olfactory function. Lipopolysaccharide- induced tumor necrosis factor (TNF)-α triggers the apoptotic cascade mediated by the TNF/TNF receptor (TNFR) pathway. The present study therefore examines the effect of the propagated innate immune reaction triggered by peripheral lipopolysaccharide on the OB of C3H/HeN mice. Within 2 h of an intraperitoneal injection of lipopolysaccharide, mRNA expression levels of the genes encoding IκB, TNF-α, and TNFR type 1 in the mouse OB were significantly enhanced. Double immunofluorescence microscopy confirmed that almost all TNF-α-immunopositive cells in the OB of the TNF-injected mice were located in the subependymal zone and that they overlapped cells immunostained with antibody against glial fibrillary acidic protein, but not with the antibody against F4/80, an antigenic marker of microglia. The number of TUNEL-positive cells identified exclusively in the granule cell layer was significantly increased in mice injected with lipopolysaccharide and sacrificed at 24 h thereafter. These results suggest that peripheral lipopolysaccharide causes disequilibrium between the supply and disappearance of the cells in the OB, which might lead to olfactory dysfunction.

Original languageEnglish
Pages (from-to)116-129
Number of pages14
JournalBrain Research
Volume1039
Issue number1-2
DOIs
Publication statusPublished - 28-03-2005

Fingerprint

Olfactory Bulb
Lipopolysaccharides
Apoptosis
Tumor Necrosis Factor-alpha
Tumor Necrosis Factor Receptors
Receptors, Tumor Necrosis Factor, Type I
Inbred C3H Mouse
Antibodies
Lateral Ventricles
Glial Fibrillary Acidic Protein
In Situ Nick-End Labeling
Microglia
Intraperitoneal Injections
Fluorescence Microscopy
Gene Expression
Neurons
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Mori, Keiji ; Kaneko, Yoko S. ; Nakashima, Akira ; Nagatsu, Ikuko ; Takahashi, Hisahide ; Ota, Akira. / Peripheral lipopolysaccharide induces apoptosis in the murine olfactory bulb. In: Brain Research. 2005 ; Vol. 1039, No. 1-2. pp. 116-129.
@article{b1239a6bf4894b16a9ecb1fe3bc3b4dc,
title = "Peripheral lipopolysaccharide induces apoptosis in the murine olfactory bulb",
abstract = "The olfactory bulb (OB) is one of the few structures in the adult mammalian CNS that contains a continuous supply of newly generated neurons in the subventricular zone. Therefore, the balance between the supply of new cells and apoptosis in the OB might determine olfactory function. Lipopolysaccharide- induced tumor necrosis factor (TNF)-α triggers the apoptotic cascade mediated by the TNF/TNF receptor (TNFR) pathway. The present study therefore examines the effect of the propagated innate immune reaction triggered by peripheral lipopolysaccharide on the OB of C3H/HeN mice. Within 2 h of an intraperitoneal injection of lipopolysaccharide, mRNA expression levels of the genes encoding IκB, TNF-α, and TNFR type 1 in the mouse OB were significantly enhanced. Double immunofluorescence microscopy confirmed that almost all TNF-α-immunopositive cells in the OB of the TNF-injected mice were located in the subependymal zone and that they overlapped cells immunostained with antibody against glial fibrillary acidic protein, but not with the antibody against F4/80, an antigenic marker of microglia. The number of TUNEL-positive cells identified exclusively in the granule cell layer was significantly increased in mice injected with lipopolysaccharide and sacrificed at 24 h thereafter. These results suggest that peripheral lipopolysaccharide causes disequilibrium between the supply and disappearance of the cells in the OB, which might lead to olfactory dysfunction.",
author = "Keiji Mori and Kaneko, {Yoko S.} and Akira Nakashima and Ikuko Nagatsu and Hisahide Takahashi and Akira Ota",
year = "2005",
month = "3",
day = "28",
doi = "10.1016/j.brainres.2005.01.078",
language = "English",
volume = "1039",
pages = "116--129",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

Mori, K, Kaneko, YS, Nakashima, A, Nagatsu, I, Takahashi, H & Ota, A 2005, 'Peripheral lipopolysaccharide induces apoptosis in the murine olfactory bulb', Brain Research, vol. 1039, no. 1-2, pp. 116-129. https://doi.org/10.1016/j.brainres.2005.01.078

Peripheral lipopolysaccharide induces apoptosis in the murine olfactory bulb. / Mori, Keiji; Kaneko, Yoko S.; Nakashima, Akira; Nagatsu, Ikuko; Takahashi, Hisahide; Ota, Akira.

In: Brain Research, Vol. 1039, No. 1-2, 28.03.2005, p. 116-129.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Peripheral lipopolysaccharide induces apoptosis in the murine olfactory bulb

AU - Mori, Keiji

AU - Kaneko, Yoko S.

AU - Nakashima, Akira

AU - Nagatsu, Ikuko

AU - Takahashi, Hisahide

AU - Ota, Akira

PY - 2005/3/28

Y1 - 2005/3/28

N2 - The olfactory bulb (OB) is one of the few structures in the adult mammalian CNS that contains a continuous supply of newly generated neurons in the subventricular zone. Therefore, the balance between the supply of new cells and apoptosis in the OB might determine olfactory function. Lipopolysaccharide- induced tumor necrosis factor (TNF)-α triggers the apoptotic cascade mediated by the TNF/TNF receptor (TNFR) pathway. The present study therefore examines the effect of the propagated innate immune reaction triggered by peripheral lipopolysaccharide on the OB of C3H/HeN mice. Within 2 h of an intraperitoneal injection of lipopolysaccharide, mRNA expression levels of the genes encoding IκB, TNF-α, and TNFR type 1 in the mouse OB were significantly enhanced. Double immunofluorescence microscopy confirmed that almost all TNF-α-immunopositive cells in the OB of the TNF-injected mice were located in the subependymal zone and that they overlapped cells immunostained with antibody against glial fibrillary acidic protein, but not with the antibody against F4/80, an antigenic marker of microglia. The number of TUNEL-positive cells identified exclusively in the granule cell layer was significantly increased in mice injected with lipopolysaccharide and sacrificed at 24 h thereafter. These results suggest that peripheral lipopolysaccharide causes disequilibrium between the supply and disappearance of the cells in the OB, which might lead to olfactory dysfunction.

AB - The olfactory bulb (OB) is one of the few structures in the adult mammalian CNS that contains a continuous supply of newly generated neurons in the subventricular zone. Therefore, the balance between the supply of new cells and apoptosis in the OB might determine olfactory function. Lipopolysaccharide- induced tumor necrosis factor (TNF)-α triggers the apoptotic cascade mediated by the TNF/TNF receptor (TNFR) pathway. The present study therefore examines the effect of the propagated innate immune reaction triggered by peripheral lipopolysaccharide on the OB of C3H/HeN mice. Within 2 h of an intraperitoneal injection of lipopolysaccharide, mRNA expression levels of the genes encoding IκB, TNF-α, and TNFR type 1 in the mouse OB were significantly enhanced. Double immunofluorescence microscopy confirmed that almost all TNF-α-immunopositive cells in the OB of the TNF-injected mice were located in the subependymal zone and that they overlapped cells immunostained with antibody against glial fibrillary acidic protein, but not with the antibody against F4/80, an antigenic marker of microglia. The number of TUNEL-positive cells identified exclusively in the granule cell layer was significantly increased in mice injected with lipopolysaccharide and sacrificed at 24 h thereafter. These results suggest that peripheral lipopolysaccharide causes disequilibrium between the supply and disappearance of the cells in the OB, which might lead to olfactory dysfunction.

UR - http://www.scopus.com/inward/record.url?scp=15244350255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15244350255&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2005.01.078

DO - 10.1016/j.brainres.2005.01.078

M3 - Article

VL - 1039

SP - 116

EP - 129

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -