Peripherally injected lipopolysaccharide induces apoptosis in the subventricular zone of young adult mice

Keiji Mori, Yoko S. Kaneko, Akira Nakashima, Toshiharu Nagatsu, Ikuko Nagatsu, Akira Ota

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Because the subventricular zone (SVZ) constantly supplies newly generated neurons to the olfactory bulb (OB) along the rostral migratory stream (RMS) in adult brain, SVZ-RMS-OB axis has been thought to work as a unit. We previously reported that peripherally injected lipopolysaccharide (LPS) induces apoptosis in the OB in young adult mice. Therefore, this study was undertaken to examine whether peripherally injected LPS induces apoptotic cell death also in the SVZ. Two mouse strains were used: C3H/HeN and Toll-like receptor 4-mutated C3H/HeJ, and wild-type C57BL/6 and TNFR1 -/--2 -/-, in which the genes tumor necrosis factor receptor (TNFR)1 and TNFR2 are knocked out. Immunohistochemical study and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay done on the SVZ-RMS pathway of young adult male mice showed that peripherally injected LPS switches on the apoptotic signal by cleaving pro-caspase-3, thus possibly increasing the number of cells dying from apoptosis in these areas in adult mice. Activation of the tumor necrosis factor (TNF)-α-TNFR system played a critical role in fully inducing apoptosis in this pathway. We suggest that TNF-α was probably released not from microglia but from astrocytes in the SVZ and RMS.

Original languageEnglish
Pages (from-to)126-130
Number of pages5
JournalNeuroscience Letters
Volume481
Issue number2
DOIs
Publication statusPublished - 01-09-2010

Fingerprint

Lateral Ventricles
Lipopolysaccharides
Young Adult
Apoptosis
Olfactory Bulb
Tumor Necrosis Factor Receptors
Tumor Necrosis Factor-alpha
Receptors, Tumor Necrosis Factor, Type II
Receptors, Tumor Necrosis Factor, Type I
Toll-Like Receptor 4
DNA Nucleotidylexotransferase
Microglia
Caspase 3
Astrocytes
Cell Death
Cell Count
Neurons
Brain
Genes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Mori, Keiji ; Kaneko, Yoko S. ; Nakashima, Akira ; Nagatsu, Toshiharu ; Nagatsu, Ikuko ; Ota, Akira. / Peripherally injected lipopolysaccharide induces apoptosis in the subventricular zone of young adult mice. In: Neuroscience Letters. 2010 ; Vol. 481, No. 2. pp. 126-130.
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Peripherally injected lipopolysaccharide induces apoptosis in the subventricular zone of young adult mice. / Mori, Keiji; Kaneko, Yoko S.; Nakashima, Akira; Nagatsu, Toshiharu; Nagatsu, Ikuko; Ota, Akira.

In: Neuroscience Letters, Vol. 481, No. 2, 01.09.2010, p. 126-130.

Research output: Contribution to journalArticle

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