Abstract
Because the subventricular zone (SVZ) constantly supplies newly generated neurons to the olfactory bulb (OB) along the rostral migratory stream (RMS) in adult brain, SVZ-RMS-OB axis has been thought to work as a unit. We previously reported that peripherally injected lipopolysaccharide (LPS) induces apoptosis in the OB in young adult mice. Therefore, this study was undertaken to examine whether peripherally injected LPS induces apoptotic cell death also in the SVZ. Two mouse strains were used: C3H/HeN and Toll-like receptor 4-mutated C3H/HeJ, and wild-type C57BL/6 and TNFR1 -/--2 -/-, in which the genes tumor necrosis factor receptor (TNFR)1 and TNFR2 are knocked out. Immunohistochemical study and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay done on the SVZ-RMS pathway of young adult male mice showed that peripherally injected LPS switches on the apoptotic signal by cleaving pro-caspase-3, thus possibly increasing the number of cells dying from apoptosis in these areas in adult mice. Activation of the tumor necrosis factor (TNF)-α-TNFR system played a critical role in fully inducing apoptosis in this pathway. We suggest that TNF-α was probably released not from microglia but from astrocytes in the SVZ and RMS.
| Original language | English |
|---|---|
| Pages (from-to) | 126-130 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 481 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 01-09-2010 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
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Peripherally injected lipopolysaccharide induces apoptosis in the subventricular zone of young adult mice. / Mori, Keiji; Kaneko, Yoko S.; Nakashima, Akira; Nagatsu, Toshiharu; Nagatsu, Ikuko; Ota, Akira.
In: Neuroscience Letters, Vol. 481, No. 2, 01.09.2010, p. 126-130.Research output: Contribution to journal › Article
TY - JOUR
T1 - Peripherally injected lipopolysaccharide induces apoptosis in the subventricular zone of young adult mice
AU - Mori, Keiji
AU - Kaneko, Yoko S.
AU - Nakashima, Akira
AU - Nagatsu, Toshiharu
AU - Nagatsu, Ikuko
AU - Ota, Akira
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Because the subventricular zone (SVZ) constantly supplies newly generated neurons to the olfactory bulb (OB) along the rostral migratory stream (RMS) in adult brain, SVZ-RMS-OB axis has been thought to work as a unit. We previously reported that peripherally injected lipopolysaccharide (LPS) induces apoptosis in the OB in young adult mice. Therefore, this study was undertaken to examine whether peripherally injected LPS induces apoptotic cell death also in the SVZ. Two mouse strains were used: C3H/HeN and Toll-like receptor 4-mutated C3H/HeJ, and wild-type C57BL/6 and TNFR1 -/--2 -/-, in which the genes tumor necrosis factor receptor (TNFR)1 and TNFR2 are knocked out. Immunohistochemical study and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay done on the SVZ-RMS pathway of young adult male mice showed that peripherally injected LPS switches on the apoptotic signal by cleaving pro-caspase-3, thus possibly increasing the number of cells dying from apoptosis in these areas in adult mice. Activation of the tumor necrosis factor (TNF)-α-TNFR system played a critical role in fully inducing apoptosis in this pathway. We suggest that TNF-α was probably released not from microglia but from astrocytes in the SVZ and RMS.
AB - Because the subventricular zone (SVZ) constantly supplies newly generated neurons to the olfactory bulb (OB) along the rostral migratory stream (RMS) in adult brain, SVZ-RMS-OB axis has been thought to work as a unit. We previously reported that peripherally injected lipopolysaccharide (LPS) induces apoptosis in the OB in young adult mice. Therefore, this study was undertaken to examine whether peripherally injected LPS induces apoptotic cell death also in the SVZ. Two mouse strains were used: C3H/HeN and Toll-like receptor 4-mutated C3H/HeJ, and wild-type C57BL/6 and TNFR1 -/--2 -/-, in which the genes tumor necrosis factor receptor (TNFR)1 and TNFR2 are knocked out. Immunohistochemical study and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay done on the SVZ-RMS pathway of young adult male mice showed that peripherally injected LPS switches on the apoptotic signal by cleaving pro-caspase-3, thus possibly increasing the number of cells dying from apoptosis in these areas in adult mice. Activation of the tumor necrosis factor (TNF)-α-TNFR system played a critical role in fully inducing apoptosis in this pathway. We suggest that TNF-α was probably released not from microglia but from astrocytes in the SVZ and RMS.
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U2 - 10.1016/j.neulet.2010.06.070
DO - 10.1016/j.neulet.2010.06.070
M3 - Article
VL - 481
SP - 126
EP - 130
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -