Persistent systemic rotavirus vaccine infection in a child with X-linked severe combined immunodeficiency

Tetsushi Yoshikawa, Masaru Ihira, Yuki Higashimoto, Fumihiko Hattori, Hiroki Miura, Ken Sugata, Satoshi Komoto, Koki Taniguchi, Akihiro Iguchi, Masafumi Yamada, Tadashi Ariga

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Objective: The main aims of the present study were to elucidate the systemic group A rotavirus (RVA) infection and to clarify the genetic changes of persistent virus in the X-linked severe combined immunodeficiency (SCID) patient. Methods: RotaTeq vaccine (RV5) genotype-specific real-time reverse transcription polymerase chain reaction was used to monitor viral RNA load in serially collected serum and stool samples. Next-generation sequence analysis was used to determine the genotype of the virus by sequencing 11 gene segments. Polyacrylamide gel electrophoresis (PAGE) analysis was used to identify rearrangement of viral genes. The gene rearrangement was examined in NSP5 gene by using Sanger sequence. Results: A 7-month-old boy demonstrated chronic diarrhea following the third administration of RV5 and failure to thrive. He was diagnosed with X-linked SCID and successfully underwent cord blood transplantation. High copy numbers of RV5 genotype G1 RNA were detected in serially collected stool and serum samples and the kinetics of viral RNA loads were correlated with the degree of clinical disease. Next-generation sequence analysis revealed genetic reassortment at least between the strains WI79-9/G1P7[5] and WI79-4/G6P1A[8] in the VP7 gene and the VP4 gene among the vaccine-derived rotavirus strains. In addition, PAGE analysis suggested genetic rearrangements in several genes, and it was confirmed in the NSP5 gene by sequence analysis. Conclusions: The kinetics of RVA RNA load in serum and stool samples was consistent with the clinical course of the patient. Among five genotypes of RV5 vaccine, G1 genotype replicated well in this patient. Reassortment and rearrangements were demonstrated in persistently infected G1 genotype of RV5.

Original languageEnglish
Pages (from-to)1008-1013
Number of pages6
JournalJournal of Medical Virology
Issue number6
Publication statusPublished - 06-2019

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases


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