Personal identification from human remains by mitochondrial DNA sequencing

Hiroyoshi Koyama, Mineo Iwasa, Susumu Ohtani, Hiroshi Ohira, Tsukasa Tsuchimochi, Yoshitaka Maeno, Ichiro Isobe, Tomohiro Matsumoto, Yoshihiro Yamada, Masataka Nagao

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


The authors report four cases in which severely damaged human remains were identified by mitochondrial DNA (mtDNA) sequencing. Degraded DNA was extracted from highly adipoceratous tissues using the phenol-chloroform method and polymerase chain reaction amplified for sequencing of two hypervariable regions, hypervariable region 1 and hypervariable region 2, of mitochondrial DNA. They also sequenced these regions of blood samples that were obtained from the presumptive mother or sister of the human remains. The sequencing results were compared with each other and with the Anderson's sequence. It was concluded from the sequence data that a lower part of a body in case 1 and some organs in case 2 were from the same woman, and a human head in case 3 and a female body in case 4 were from the relative of a presumptive mother and a sister, respectively.

Original languageEnglish
Pages (from-to)272-276
Number of pages5
JournalAmerican Journal of Forensic Medicine and Pathology
Issue number3
Publication statusPublished - 09-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine


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