Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line

Seiji Miyake; Saori Kobayashi; Kazuo Tsubota; Yoko Ozawa

doi:10.1016/j.bbrc.2014.02.135

Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line

Seiji Miyake, Saori Kobayashi, Kazuo Tsubota, Yoko Ozawa

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina.

Original language	English
Pages (from-to)	535-540
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	446
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2014.02.135
Publication status	Published - 04-04-2014
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2014.02.135

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abstract = "The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina.",

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AU - Miyake, Seiji

AU - Kobayashi, Saori

AU - Tsubota, Kazuo

AU - Ozawa, Yoko

PY - 2014/4/4

Y1 - 2014/4/4

N2 - The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina.

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Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line

Abstract

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