Phenotypic changes of AADC-only-immunoreactive cells in the alimentary canal of the laboratory shrew, Suncus murinus, induced by systemic administration of monoamine precursors

Kazuyoshi Sakai, Ryuji Nomura, Yoshimi Hasegawa, Masanori Sinzato, Kazuhiro Nishii, Yoshimitsu Katoh, Koji Yamada

Research output: Contribution to journalArticle

Abstract

In order to elucidate the function of anti-aromatic acid decarboxylase (AADC)-only-positive cells in the alimentary canal, 5-hydroxy-L-tryptophan (5-HTP) or L-3,4-dihydroxyphenylalanine (L-DOPA) was intraperitoneally injected into the laboratory shrew, Suncus murinus, and immunohistochemical studies were conducted on continuous sections of the alimentary canal using specific antisera against tyrosine hydroxylase (TH), AADC, dopamine (DA), and serotonin (5-HT). AADC-only-positive cells localized to the epithelial layer of the alimentary canal from the stomach to the large intestine. These AADC-only-positive cells became DA- and AADC-positive cells after L-DOPA injection, and 5-HT- and AADC-positive cells after 5-HTP injection. These results strongly indicate that the AADC-only-positive cells in the alimentary canal of Suncus murinus are capable of synthesizing DA and 5-HT simultaneously upon administration of L-DOPA and 5-HTP.

Original languageEnglish
Pages (from-to)43-47
Number of pages5
JournalOkajimas Folia Anatomica Japonica
Volume92
Issue number2
DOIs
Publication statusPublished - 01-01-2015

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Shrews
Carboxy-Lyases
Acids
5-Hydroxytryptophan
Serotonin
Levodopa
Tryptophan
Dopamine
Injections
Large Intestine
Tyrosine 3-Monooxygenase
Immune Sera
Stomach

All Science Journal Classification (ASJC) codes

  • Anatomy

Cite this

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title = "Phenotypic changes of AADC-only-immunoreactive cells in the alimentary canal of the laboratory shrew, Suncus murinus, induced by systemic administration of monoamine precursors",
abstract = "In order to elucidate the function of anti-aromatic acid decarboxylase (AADC)-only-positive cells in the alimentary canal, 5-hydroxy-L-tryptophan (5-HTP) or L-3,4-dihydroxyphenylalanine (L-DOPA) was intraperitoneally injected into the laboratory shrew, Suncus murinus, and immunohistochemical studies were conducted on continuous sections of the alimentary canal using specific antisera against tyrosine hydroxylase (TH), AADC, dopamine (DA), and serotonin (5-HT). AADC-only-positive cells localized to the epithelial layer of the alimentary canal from the stomach to the large intestine. These AADC-only-positive cells became DA- and AADC-positive cells after L-DOPA injection, and 5-HT- and AADC-positive cells after 5-HTP injection. These results strongly indicate that the AADC-only-positive cells in the alimentary canal of Suncus murinus are capable of synthesizing DA and 5-HT simultaneously upon administration of L-DOPA and 5-HTP.",
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Phenotypic changes of AADC-only-immunoreactive cells in the alimentary canal of the laboratory shrew, Suncus murinus, induced by systemic administration of monoamine precursors. / Sakai, Kazuyoshi; Nomura, Ryuji; Hasegawa, Yoshimi; Sinzato, Masanori; Nishii, Kazuhiro; Katoh, Yoshimitsu; Yamada, Koji.

In: Okajimas Folia Anatomica Japonica, Vol. 92, No. 2, 01.01.2015, p. 43-47.

Research output: Contribution to journalArticle

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T1 - Phenotypic changes of AADC-only-immunoreactive cells in the alimentary canal of the laboratory shrew, Suncus murinus, induced by systemic administration of monoamine precursors

AU - Sakai, Kazuyoshi

AU - Nomura, Ryuji

AU - Hasegawa, Yoshimi

AU - Sinzato, Masanori

AU - Nishii, Kazuhiro

AU - Katoh, Yoshimitsu

AU - Yamada, Koji

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