Phosphatidylserine synthesis required for the maximal tryptophan transport activity in Saccharomyces cerevisiae

Hidemitsu Nakamura, Keiji Miura, Yu Fukuda, Isao Shibuya, Akinori Ohta, Masamichi Takagi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Saccharomyces cerevisiae cho1/pss mutants, which are severely impaired in phosphatidylserine (PS) synthesis, do not have detectable amounts of PS in their lipid fractions. Their derivatives with mutations that cause defects in tryptophan synthesis grew poorly in a medium containing 5 μg/ml of L-tryptophan, a concentration that met the requirements of tryptophan-auxotrophic CHO1/PSS strains. The rates of tryptophan uptake of trp1 cho1/pss mutants were low at low tryptophan concentrations. This defect in the use of tryptophan was restored either by expression of CHO1/PSS or by introduction of a gene encoding tryptophan transporter, TAT1 or TAT2. These results indicate that PS synthesis is required for the maximal tryptophan-transporting activity of S. cerevisiae at low tryptophan concentrations.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number1
DOIs
Publication statusPublished - 01-2000

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Phosphatidylserine synthesis required for the maximal tryptophan transport activity in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this