Abstract
Ultraviolet (UV)-B radiation acts as an elicitor to enhance the production of secondary metabolites in medicinal plants. To investigate the mechanisms, which lead to secondary metabolites in Catharanthus roseus under UVB radiation, a phosphoproteomic technique was used. ATP content increased in the leaves of C. roseus under UVB radiation. Phosphoproteins related to calcium such as calmodulin, calcium-dependent kinase, and heat shock proteins increased. Phosphoproteins related to protein synthesis/modification/degradation and signaling intensively changed. Metabolomic analysis indicated that the metabolites classified with pentoses, aromatic amino acids, and phenylpropanoids accumulated under UVB radiation. Phosphoproteomic and immunoblot analyses indicated that proteins related to glycolysis and the reactive-oxygen species scavenging system were changed under UVB radiation. These results suggest that UVB radiation activates the calcium-related pathway and reactive-oxygen species scavenging system in C. roseus. These changes lead to the upregulation of proteins, which are responsible for the redox reactions in secondary metabolism and are important for the accumulation of secondary metabolites in C. roseus under UVB radiation.
| Original language | English |
|---|---|
| Pages (from-to) | 3328-3341 |
| Number of pages | 14 |
| Journal | Journal of Proteome Research |
| Volume | 18 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 06-09-2019 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Chemistry(all)
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Phosphoproteomics Reveals the Biosynthesis of Secondary Metabolites in Catharanthus roseus under Ultraviolet-B Radiation. / Zhong, Zhuoheng; Liu, Shengzhi; Zhu, Wei; Ou, Yuting; Yamaguchi, Hisateru; Hitachi, Keisuke; Tsuchida, Kunihiro; Tian, Jingkui; Komatsu, Setsuko.
In: Journal of Proteome Research, Vol. 18, No. 9, 06.09.2019, p. 3328-3341.Research output: Contribution to journal › Article
TY - JOUR
T1 - Phosphoproteomics Reveals the Biosynthesis of Secondary Metabolites in Catharanthus roseus under Ultraviolet-B Radiation
AU - Zhong, Zhuoheng
AU - Liu, Shengzhi
AU - Zhu, Wei
AU - Ou, Yuting
AU - Yamaguchi, Hisateru
AU - Hitachi, Keisuke
AU - Tsuchida, Kunihiro
AU - Tian, Jingkui
AU - Komatsu, Setsuko
PY - 2019/9/6
Y1 - 2019/9/6
N2 - Ultraviolet (UV)-B radiation acts as an elicitor to enhance the production of secondary metabolites in medicinal plants. To investigate the mechanisms, which lead to secondary metabolites in Catharanthus roseus under UVB radiation, a phosphoproteomic technique was used. ATP content increased in the leaves of C. roseus under UVB radiation. Phosphoproteins related to calcium such as calmodulin, calcium-dependent kinase, and heat shock proteins increased. Phosphoproteins related to protein synthesis/modification/degradation and signaling intensively changed. Metabolomic analysis indicated that the metabolites classified with pentoses, aromatic amino acids, and phenylpropanoids accumulated under UVB radiation. Phosphoproteomic and immunoblot analyses indicated that proteins related to glycolysis and the reactive-oxygen species scavenging system were changed under UVB radiation. These results suggest that UVB radiation activates the calcium-related pathway and reactive-oxygen species scavenging system in C. roseus. These changes lead to the upregulation of proteins, which are responsible for the redox reactions in secondary metabolism and are important for the accumulation of secondary metabolites in C. roseus under UVB radiation.
AB - Ultraviolet (UV)-B radiation acts as an elicitor to enhance the production of secondary metabolites in medicinal plants. To investigate the mechanisms, which lead to secondary metabolites in Catharanthus roseus under UVB radiation, a phosphoproteomic technique was used. ATP content increased in the leaves of C. roseus under UVB radiation. Phosphoproteins related to calcium such as calmodulin, calcium-dependent kinase, and heat shock proteins increased. Phosphoproteins related to protein synthesis/modification/degradation and signaling intensively changed. Metabolomic analysis indicated that the metabolites classified with pentoses, aromatic amino acids, and phenylpropanoids accumulated under UVB radiation. Phosphoproteomic and immunoblot analyses indicated that proteins related to glycolysis and the reactive-oxygen species scavenging system were changed under UVB radiation. These results suggest that UVB radiation activates the calcium-related pathway and reactive-oxygen species scavenging system in C. roseus. These changes lead to the upregulation of proteins, which are responsible for the redox reactions in secondary metabolism and are important for the accumulation of secondary metabolites in C. roseus under UVB radiation.
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UR - http://www.scopus.com/inward/citedby.url?scp=85070946987&partnerID=8YFLogxK
U2 - 10.1021/acs.jproteome.9b00267
DO - 10.1021/acs.jproteome.9b00267
M3 - Article
C2 - 31356092
AN - SCOPUS:85070946987
VL - 18
SP - 3328
EP - 3341
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 9
ER -