Phosphoproteomics Reveals the Biosynthesis of Secondary Metabolites in Catharanthus roseus under Ultraviolet-B Radiation

Zhuoheng Zhong, Shengzhi Liu, Wei Zhu, Yuting Ou, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida, Jingkui Tian, Setsuko Komatsu

Research output: Contribution to journalArticle

Abstract

Ultraviolet (UV)-B radiation acts as an elicitor to enhance the production of secondary metabolites in medicinal plants. To investigate the mechanisms, which lead to secondary metabolites in Catharanthus roseus under UVB radiation, a phosphoproteomic technique was used. ATP content increased in the leaves of C. roseus under UVB radiation. Phosphoproteins related to calcium such as calmodulin, calcium-dependent kinase, and heat shock proteins increased. Phosphoproteins related to protein synthesis/modification/degradation and signaling intensively changed. Metabolomic analysis indicated that the metabolites classified with pentoses, aromatic amino acids, and phenylpropanoids accumulated under UVB radiation. Phosphoproteomic and immunoblot analyses indicated that proteins related to glycolysis and the reactive-oxygen species scavenging system were changed under UVB radiation. These results suggest that UVB radiation activates the calcium-related pathway and reactive-oxygen species scavenging system in C. roseus. These changes lead to the upregulation of proteins, which are responsible for the redox reactions in secondary metabolism and are important for the accumulation of secondary metabolites in C. roseus under UVB radiation.

Original languageEnglish
Pages (from-to)3328-3341
Number of pages14
JournalJournal of Proteome Research
Volume18
Issue number9
DOIs
Publication statusPublished - 06-09-2019

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Catharanthus
Biosynthesis
Metabolites
Radiation
Phosphoproteins
Scavenging
Calcium
Reactive Oxygen Species
Secondary Metabolism
Pentoses
Aromatic Amino Acids
Proteins
Metabolomics
Redox reactions
Glycolysis
Calmodulin
Medicinal Plants
Heat-Shock Proteins
Oxidation-Reduction
Phosphotransferases

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

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title = "Phosphoproteomics Reveals the Biosynthesis of Secondary Metabolites in Catharanthus roseus under Ultraviolet-B Radiation",
abstract = "Ultraviolet (UV)-B radiation acts as an elicitor to enhance the production of secondary metabolites in medicinal plants. To investigate the mechanisms, which lead to secondary metabolites in Catharanthus roseus under UVB radiation, a phosphoproteomic technique was used. ATP content increased in the leaves of C. roseus under UVB radiation. Phosphoproteins related to calcium such as calmodulin, calcium-dependent kinase, and heat shock proteins increased. Phosphoproteins related to protein synthesis/modification/degradation and signaling intensively changed. Metabolomic analysis indicated that the metabolites classified with pentoses, aromatic amino acids, and phenylpropanoids accumulated under UVB radiation. Phosphoproteomic and immunoblot analyses indicated that proteins related to glycolysis and the reactive-oxygen species scavenging system were changed under UVB radiation. These results suggest that UVB radiation activates the calcium-related pathway and reactive-oxygen species scavenging system in C. roseus. These changes lead to the upregulation of proteins, which are responsible for the redox reactions in secondary metabolism and are important for the accumulation of secondary metabolites in C. roseus under UVB radiation.",
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Phosphoproteomics Reveals the Biosynthesis of Secondary Metabolites in Catharanthus roseus under Ultraviolet-B Radiation. / Zhong, Zhuoheng; Liu, Shengzhi; Zhu, Wei; Ou, Yuting; Yamaguchi, Hisateru; Hitachi, Keisuke; Tsuchida, Kunihiro; Tian, Jingkui; Komatsu, Setsuko.

In: Journal of Proteome Research, Vol. 18, No. 9, 06.09.2019, p. 3328-3341.

Research output: Contribution to journalArticle

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AU - Zhong, Zhuoheng

AU - Liu, Shengzhi

AU - Zhu, Wei

AU - Ou, Yuting

AU - Yamaguchi, Hisateru

AU - Hitachi, Keisuke

AU - Tsuchida, Kunihiro

AU - Tian, Jingkui

AU - Komatsu, Setsuko

PY - 2019/9/6

Y1 - 2019/9/6

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