Phosphorylation of moesin by Rho-associated kinase (Rho-kinase) plays a crucial role in the formation of microvilli-like structures

Noriko Oshiro, Yuko Fukata, Kozo Kaibuchi

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

Rho-associated kinase (Rho-kinase), which is activated by the small GTPase Rho, phosphorylates moesin at Thr558 in vitro. Here, using a site- and phosphorylation state-specific antibody, we found that the expression of dominant active RhoA in COS7 cells induced moesin phosphorylation and the formation of microvilli-like structures at apical membranes where the Thr558-phosphorylated moesin accumulated, whereas the expression of dominant negative Rho-kinase inhibited both of these processes. The expression of dominant active Rho-kinase also induced moesin phosphorylation. When COS7 cells expressing moesin or moesin(T558A) (substitution of Thr by Ala) were cultured under serum-depleted conditions, there were few microvilli-like structures, whereas microvilli-like structures remained in the cells expressing moesin(T558D) (substitution of Thr by Asp). The expression of moesin(T558A) inhibited the dominant active RhoA-induced formation of microvilli-like structures. These results indicate that Rho- kinase regulates moesin phosphorylation downstream of Rho in vivo and that the phosphorylation of moesin by Rhokinase plays a crucial role in the formation of microvilli-like structures.

Original languageEnglish
Pages (from-to)34663-34666
Number of pages4
JournalJournal of Biological Chemistry
Volume273
Issue number52
DOIs
Publication statusPublished - 25-12-1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Phosphorylation of moesin by Rho-associated kinase (Rho-kinase) plays a crucial role in the formation of microvilli-like structures'. Together they form a unique fingerprint.

Cite this