TY - JOUR
T1 - Phosphorylation of transcriptional regulators in the retinoblastoma protein pathway by UL97, the viral cyclin-dependent kinase encoded by human cytomegalovirus
AU - Iwahori, Satoko
AU - Kalejta, Robert F.
N1 - Funding Information:
We thank our lab managers Phil Balandyk and Diccon Fiore for expert technical assistance, the members of our lab for helpful discussion, and these investigators for providing research materials: Don Coen, Jim DeCapprio, Greg Enders, Ed Harlow, Philip Hinds, Jacqueline Lees, Larisa Litovchick, Joe Nevins, Jonathon Pines, Pradip Raychaudhuri, and Sander van den Heuvel. SI was supported by a Japan Herpesvirus Infections Forum scholarship award in herpesvirus infection research. This work was supported by grants from the NIH to RFK ( R01-AI080675 ) and Paul Lambert ( P01-CA022443 ).
PY - 2017/12
Y1 - 2017/12
N2 - Human cytomegalovirus (HCMV) encodes a viral cyclin-dependent kinase (v-CDK), the UL97 protein. UL97 phosphorylates Rb, p107 and p130, thereby inactivating all three retinoblastoma (Rb) family members. Rb proteins function through regulating the activity of transcription factors to which they bind. Therefore, we examined whether the UL97-mediated regulation of the Rb tumor suppressors also extended to their binding partners. We observed that UL97 phosphorylates LIN52, a component of p107- and p130-assembled transcriptionally repressive DREAM complexes that control transcription during the G0/G1 phases, and the Rb-associated E2F3 protein that activates transcription through G1 and S phases. Intriguingly, we also identified FoxM1B, a transcriptional regulator during the S and G2 phases, as a UL97 substrate. This survey extends the influence of UL97 beyond simply the Rb proteins themselves to their binding partners, as well as past the G1/S transition into later stages of the cell cycle.
AB - Human cytomegalovirus (HCMV) encodes a viral cyclin-dependent kinase (v-CDK), the UL97 protein. UL97 phosphorylates Rb, p107 and p130, thereby inactivating all three retinoblastoma (Rb) family members. Rb proteins function through regulating the activity of transcription factors to which they bind. Therefore, we examined whether the UL97-mediated regulation of the Rb tumor suppressors also extended to their binding partners. We observed that UL97 phosphorylates LIN52, a component of p107- and p130-assembled transcriptionally repressive DREAM complexes that control transcription during the G0/G1 phases, and the Rb-associated E2F3 protein that activates transcription through G1 and S phases. Intriguingly, we also identified FoxM1B, a transcriptional regulator during the S and G2 phases, as a UL97 substrate. This survey extends the influence of UL97 beyond simply the Rb proteins themselves to their binding partners, as well as past the G1/S transition into later stages of the cell cycle.
UR - http://www.scopus.com/inward/record.url?scp=85029708328&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029708328&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2017.09.009
DO - 10.1016/j.virol.2017.09.009
M3 - Article
C2 - 28946006
AN - SCOPUS:85029708328
VL - 512
SP - 95
EP - 103
JO - Virology
JF - Virology
SN - 0042-6822
ER -