Physical interaction between Tbx6 and mespb is indispensable for the activation of bowline expression during Xenopus somitogenesis

Keisuke Hitachi, Hiroki Danno, Akiko Kondow, Kiyoshi Ohnuma, Hideho Uchiyama, Shoichi Ishiura, Akira Kurisaki, Makoto Asashima

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During vertebrate somitogenesis, various transcriptional factors function coordinately to determine the position of the somite boundary. Previously, we reported on the signaling crosstalk that occurs between two major transcription factors involved in somitogenesis, Tbx6 and mespb/mesp2. These factors synergistically activated the expression of a downstream gene, bowline/Ripply2, which is essential for precise formation of the somite boundary. However, the molecular mechanism underlying this synergistic effect remains unclear. In this report, we found that the Tbx6 and mespb proteins interacted physically with each other. Pulldown assays with various deletion mutants of these proteins identified the essential domains for this physical interaction. Finally, we found that interference with the physical interaction by a dominant-negative form of mespb, mespbΔDBD, abrogated the expression of the bowline gene during Xenopus somitogenesis. These results indicate that the appropriate expression of bowline/Ripply2 is regulated by a direct interaction between the Tbx6 and mespb proteins during Xenopus somitogenesis.

Original languageEnglish
Pages (from-to)607-612
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume372
Issue number4
DOIs
Publication statusPublished - 08-08-2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Physical interaction between Tbx6 and mespb is indispensable for the activation of bowline expression during Xenopus somitogenesis'. Together they form a unique fingerprint.

  • Cite this