PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a

Mami Yamaoka, Tomomi Ando, Takeshi Terabayashi, Mitsuhiro Okamoto, Masahiro Takei, Tomoki Nishioka, Kozo Kaibuchi, Kohichi Matsunaga, Ray Ishizaki, Tetsuro Izumi, Ichiro Niki, Toshimasa Ishizaki, Toshihide Kimura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In secretory cells, endocytosis is coupled to exocytosis to enable proper secretion. Although endocytosis is crucial to maintain cellular homeostasis before and after secretion, knowledge about secretagogue-induced endocytosis in secretory cells is still limited. Here, we searched for proteins that interacted with the Rab27a GTPase-activating protein (GAP) EPI64 (also known as TBC1D10A) and identified the Arf6 guanine-nucleotide-exchange factor (GEF) ARNO (also known as CYTH2) in pancreatic β-cells. We found that the insulin secretagogue glucose promotes phosphatidylinositol (3,4,5)-trisphosphate (PIP3) generation through phosphoinositide 3-kinase (PI3K), thereby recruiting ARNO to the intracellular side of the plasma membrane. Peripheral ARNO promotes clathrin assembly through its GEF activity for Arf6 and regulates the early stage of endocytosis. We also found that peripheral ARNO recruits EPI64 to the same area and that the interaction requires glucose-induced endocytosis in pancreatic β-cells. Given that GTP- and GDP-bound Rab27a regulate exocytosis and the late stage of endocytosis, our results indicate that the glucose-induced activation of PI3K plays a pivotal role in exocytosis-endocytosis coupling, and that ARNO and EPI64 regulate endocytosis at distinct stages.

Original languageEnglish
Pages (from-to)637-649
Number of pages13
JournalJournal of cell science
Volume129
Issue number3
DOIs
Publication statusPublished - 01-01-2016

All Science Journal Classification (ASJC) codes

  • Cell Biology

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    Yamaoka, M., Ando, T., Terabayashi, T., Okamoto, M., Takei, M., Nishioka, T., Kaibuchi, K., Matsunaga, K., Ishizaki, R., Izumi, T., Niki, I., Ishizaki, T., & Kimura, T. (2016). PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a. Journal of cell science, 129(3), 637-649. https://doi.org/10.1242/jcs.180141