Pilot study of probe-based confocal laser endomicroscopy with fluorescein-dripping method for liver tumors

Masaki Morimoto; Soichiro Honjo; Teruhisa Sakamoto; Ei Uchinaka; Masataka Amisaki; Yosuke Arai; Manabu Yamamoto; Yoji Fukumoto; Tomohiro Osaki; Naruo Tokuyasu; Keigo Ashida; Hiroaki Saito; Yoshiyuki Fujiwara

doi:10.21873/anticanres.12786

Pilot study of probe-based confocal laser endomicroscopy with fluorescein-dripping method for liver tumors

Masaki Morimoto, Soichiro Honjo, Teruhisa Sakamoto, Ei Uchinaka, Masataka Amisaki, Yosuke Arai, Manabu Yamamoto, Yoji Fukumoto, Tomohiro Osaki, Naruo Tokuyasu, Keigo Ashida, Hiroaki Saito, Yoshiyuki Fujiwara

Gastrointestinal Surgery

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Background/Aim: Probe-based confocal laser endomicroscopy (pCLE) is a novel diagnostic technique that can provide real-time imaging of tissues at the cellular level. In this study, pCLE was applied to liver tumors and its diagnostic value was evaluated. Patients and Methods: Ten patients who underwent hepatectomy for liver tumors were evaluated with pCLE from February to May 2017. Immediately after liver resection, pCLE evaluation was performed in the operating room as an ex vivo study. The newly-adopted "fluorescein-dripping method" was used for the observation. Results: First, the optimal fluorescein exposure time for the surface of liver was defined in the fluorescein-dripping method. Next, the distinctive findings in the cancerous region were investigated. The characteristic appearance of irregular arrangements of concentrated cells under fluorescein dripping was observed in six of seven hepatocellular carcinoma (HCC) tumors. Conclusion: In all HCC specimens, discrimination of the cancerous region from normal liver was possible with pCLE.

Original language	English
Pages (from-to)	4775-4781
Number of pages	7
Journal	Anticancer research
Volume	38
Issue number	8
DOIs	https://doi.org/10.21873/anticanres.12786
Publication status	Published - 08-2018

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Oncology
Cancer Research

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10.21873/anticanres.12786

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title = "Pilot study of probe-based confocal laser endomicroscopy with fluorescein-dripping method for liver tumors",

abstract = "Background/Aim: Probe-based confocal laser endomicroscopy (pCLE) is a novel diagnostic technique that can provide real-time imaging of tissues at the cellular level. In this study, pCLE was applied to liver tumors and its diagnostic value was evaluated. Patients and Methods: Ten patients who underwent hepatectomy for liver tumors were evaluated with pCLE from February to May 2017. Immediately after liver resection, pCLE evaluation was performed in the operating room as an ex vivo study. The newly-adopted {"}fluorescein-dripping method{"} was used for the observation. Results: First, the optimal fluorescein exposure time for the surface of liver was defined in the fluorescein-dripping method. Next, the distinctive findings in the cancerous region were investigated. The characteristic appearance of irregular arrangements of concentrated cells under fluorescein dripping was observed in six of seven hepatocellular carcinoma (HCC) tumors. Conclusion: In all HCC specimens, discrimination of the cancerous region from normal liver was possible with pCLE.",

keywords = "Confocal laser endomicroscopy, Fluorescein, Hepatocellular carcinoma, Liver tumor",

author = "Masaki Morimoto and Soichiro Honjo and Teruhisa Sakamoto and Ei Uchinaka and Masataka Amisaki and Yosuke Arai and Manabu Yamamoto and Yoji Fukumoto and Tomohiro Osaki and Naruo Tokuyasu and Keigo Ashida and Hiroaki Saito and Yoshiyuki Fujiwara",

year = "2018",

month = aug,

doi = "10.21873/anticanres.12786",

language = "English",

volume = "38",

pages = "4775--4781",

journal = "Anticancer research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "8",

}

TY - JOUR

T1 - Pilot study of probe-based confocal laser endomicroscopy with fluorescein-dripping method for liver tumors

AU - Morimoto, Masaki

AU - Honjo, Soichiro

AU - Sakamoto, Teruhisa

AU - Uchinaka, Ei

AU - Amisaki, Masataka

AU - Arai, Yosuke

AU - Yamamoto, Manabu

AU - Fukumoto, Yoji

AU - Osaki, Tomohiro

AU - Tokuyasu, Naruo

AU - Ashida, Keigo

AU - Saito, Hiroaki

AU - Fujiwara, Yoshiyuki

PY - 2018/8

Y1 - 2018/8

N2 - Background/Aim: Probe-based confocal laser endomicroscopy (pCLE) is a novel diagnostic technique that can provide real-time imaging of tissues at the cellular level. In this study, pCLE was applied to liver tumors and its diagnostic value was evaluated. Patients and Methods: Ten patients who underwent hepatectomy for liver tumors were evaluated with pCLE from February to May 2017. Immediately after liver resection, pCLE evaluation was performed in the operating room as an ex vivo study. The newly-adopted "fluorescein-dripping method" was used for the observation. Results: First, the optimal fluorescein exposure time for the surface of liver was defined in the fluorescein-dripping method. Next, the distinctive findings in the cancerous region were investigated. The characteristic appearance of irregular arrangements of concentrated cells under fluorescein dripping was observed in six of seven hepatocellular carcinoma (HCC) tumors. Conclusion: In all HCC specimens, discrimination of the cancerous region from normal liver was possible with pCLE.

AB - Background/Aim: Probe-based confocal laser endomicroscopy (pCLE) is a novel diagnostic technique that can provide real-time imaging of tissues at the cellular level. In this study, pCLE was applied to liver tumors and its diagnostic value was evaluated. Patients and Methods: Ten patients who underwent hepatectomy for liver tumors were evaluated with pCLE from February to May 2017. Immediately after liver resection, pCLE evaluation was performed in the operating room as an ex vivo study. The newly-adopted "fluorescein-dripping method" was used for the observation. Results: First, the optimal fluorescein exposure time for the surface of liver was defined in the fluorescein-dripping method. Next, the distinctive findings in the cancerous region were investigated. The characteristic appearance of irregular arrangements of concentrated cells under fluorescein dripping was observed in six of seven hepatocellular carcinoma (HCC) tumors. Conclusion: In all HCC specimens, discrimination of the cancerous region from normal liver was possible with pCLE.

KW - Confocal laser endomicroscopy

KW - Fluorescein

KW - Hepatocellular carcinoma

KW - Liver tumor

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M3 - Article

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ER -

Pilot study of probe-based confocal laser endomicroscopy with fluorescein-dripping method for liver tumors

Abstract

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