Pin1 Interacts with the Epstein-Barr Virus DNA Polymerase Catalytic Subunit and Regulates Viral DNA Replication

Yohei Narita, Takayuki Murata, Akihide Ryo, Daisuke Kawashima, Atsuko Sugimoto, Teru Kanda, Hiroshi Kimura, Tatsuya Tsurumi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) protein is known as a regulator which recognizes phosphorylated Ser/Thr-Pro motifs and increases the rate of cis and trans amide isomer interconversion, thereby altering the conformation of its substrates. We found that Pin1 knockdown using short hairpin RNA (shRNA) technology resulted in strong suppression of productive Epstein-Barr virus (EBV) DNA replication. We further identified the EBV DNA polymerase catalytic subunit, BALF5, as a Pin1 substrate in glutathione S-transferase (GST) pulldown and immunoprecipitation assays. Lambda protein phosphatase treatment abolished the binding of BALF5 to Pin1, and mutation analysis of BALF5 revealed that replacement of the Thr178 residue by Ala (BALF5 T178A) disrupted the interaction with Pin1. To further test the effects of Pin1 in the context of virus infection, we constructed a BALF5-deficient recombinant virus. Exogenous supply of wild-type BALF5 in HEK293 cells with knockout recombinant EBV allowed efficient synthesis of viral genome DNA, but BALF5 T178A could not provide support as efficiently as wild-type BALF5. In conclusion, we found that EBV DNA polymerase BALF5 subunit interacts with Pin1 through BALF5 Thr178 in a phosphorylation-dependent manner. Pin1 might modulate EBV DNA polymerase conformation for efficient, productive viral DNA replication.

Original languageEnglish
Pages (from-to)2120-2127
Number of pages8
JournalJournal of Virology
Volume87
Issue number4
DOIs
Publication statusPublished - 01-02-2013

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Human herpesvirus 4
Viral DNA
DNA-directed DNA polymerase
DNA replication
protein subunits
DNA Replication
Catalytic Domain
Human Herpesvirus 4
Peptidylprolyl Isomerase
Nucleic Acid Conformation
HEK293 Cells
Viral Genome
Phosphoprotein Phosphatases
Virus Diseases
Virus Replication
Glutathione Transferase
Immunoprecipitation
Amides
Small Interfering RNA
peptidylprolyl isomerase

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Narita, Yohei ; Murata, Takayuki ; Ryo, Akihide ; Kawashima, Daisuke ; Sugimoto, Atsuko ; Kanda, Teru ; Kimura, Hiroshi ; Tsurumi, Tatsuya. / Pin1 Interacts with the Epstein-Barr Virus DNA Polymerase Catalytic Subunit and Regulates Viral DNA Replication. In: Journal of Virology. 2013 ; Vol. 87, No. 4. pp. 2120-2127.
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Narita, Y, Murata, T, Ryo, A, Kawashima, D, Sugimoto, A, Kanda, T, Kimura, H & Tsurumi, T 2013, 'Pin1 Interacts with the Epstein-Barr Virus DNA Polymerase Catalytic Subunit and Regulates Viral DNA Replication', Journal of Virology, vol. 87, no. 4, pp. 2120-2127. https://doi.org/10.1128/JVI.02634-12

Pin1 Interacts with the Epstein-Barr Virus DNA Polymerase Catalytic Subunit and Regulates Viral DNA Replication. / Narita, Yohei; Murata, Takayuki; Ryo, Akihide; Kawashima, Daisuke; Sugimoto, Atsuko; Kanda, Teru; Kimura, Hiroshi; Tsurumi, Tatsuya.

In: Journal of Virology, Vol. 87, No. 4, 01.02.2013, p. 2120-2127.

Research output: Contribution to journalArticle

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