Pituitary adenylate cyclase-activating polypeptide prevents cytokine-induced cytotoxicity via inhibition of inducible nitric oxide synthase expression in βTC cells

Kayo Sekiya, Hiroshi Nagasaki, Nobuaki Ozaki, Atsushi Suzuki, Yoshitaka Miura, Yutaka Oiso

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Type 1 diabetes mellitus is an autoimmune disease resulting from apoptotic destruction of pancreatic β-cells. The activation of inducible nitric oxide synthase (iNOS) by inflammatory cytokines is considered a mediator of destruction in β-cells. Recent findings showed that the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP), whose distribution was identified in pancreatic neurons, inhibited nitric oxide (NO) production in cytokine-activated macrophages. In the present study, we investigated the cytoprotective effect of PACAP in the cytokine-exposed mice β-cell line, βTC cells. 1 x 10 -8 M PACAP inhibited the reduction of cell viability, NO production, expression of iNOS mRNA, and iNOS promoter activity caused by the combination of three proinflammatory cytokines. Selective iNOS inhibitor also showed the cytoprotective effect in βTC cells. These data suggested that PACAP has a cytoprotective effect in cytokine-treated β-cells through inhibition of iNOS transcription. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume278
Issue number1
DOIs
Publication statusPublished - 11-11-2000
Externally publishedYes

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Nitric Oxide Synthase Type II
Cytotoxicity
Cytokines
Nitric Oxide
Cells
Macrophages
Transcription
Medical problems
Neuropeptides
Type 1 Diabetes Mellitus
Autoimmune Diseases
Neurons
Cell Survival
Chemical activation
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Type 1 diabetes mellitus is an autoimmune disease resulting from apoptotic destruction of pancreatic β-cells. The activation of inducible nitric oxide synthase (iNOS) by inflammatory cytokines is considered a mediator of destruction in β-cells. Recent findings showed that the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP), whose distribution was identified in pancreatic neurons, inhibited nitric oxide (NO) production in cytokine-activated macrophages. In the present study, we investigated the cytoprotective effect of PACAP in the cytokine-exposed mice β-cell line, βTC cells. 1 x 10 -8 M PACAP inhibited the reduction of cell viability, NO production, expression of iNOS mRNA, and iNOS promoter activity caused by the combination of three proinflammatory cytokines. Selective iNOS inhibitor also showed the cytoprotective effect in βTC cells. These data suggested that PACAP has a cytoprotective effect in cytokine-treated β-cells through inhibition of iNOS transcription. (C) 2000 Academic Press.",
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Pituitary adenylate cyclase-activating polypeptide prevents cytokine-induced cytotoxicity via inhibition of inducible nitric oxide synthase expression in βTC cells. / Sekiya, Kayo; Nagasaki, Hiroshi; Ozaki, Nobuaki; Suzuki, Atsushi; Miura, Yoshitaka; Oiso, Yutaka.

In: Biochemical and Biophysical Research Communications, Vol. 278, No. 1, 11.11.2000, p. 211-216.

Research output: Contribution to journalArticle

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AU - Sekiya, Kayo

AU - Nagasaki, Hiroshi

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AU - Miura, Yoshitaka

AU - Oiso, Yutaka

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AB - Type 1 diabetes mellitus is an autoimmune disease resulting from apoptotic destruction of pancreatic β-cells. The activation of inducible nitric oxide synthase (iNOS) by inflammatory cytokines is considered a mediator of destruction in β-cells. Recent findings showed that the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP), whose distribution was identified in pancreatic neurons, inhibited nitric oxide (NO) production in cytokine-activated macrophages. In the present study, we investigated the cytoprotective effect of PACAP in the cytokine-exposed mice β-cell line, βTC cells. 1 x 10 -8 M PACAP inhibited the reduction of cell viability, NO production, expression of iNOS mRNA, and iNOS promoter activity caused by the combination of three proinflammatory cytokines. Selective iNOS inhibitor also showed the cytoprotective effect in βTC cells. These data suggested that PACAP has a cytoprotective effect in cytokine-treated β-cells through inhibition of iNOS transcription. (C) 2000 Academic Press.

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