Plasmodium knowlesi and human malaria parasites in Khan Phu, Vietnam

Gametocyte production in humans and frequent co-infection of mosquitoes

Yoshimasa Maeno, R. Culleton, N. T. Quang, S. Kawai, R. P. Marchand, S. Nakazawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Four species of malaria parasite, Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium knowlesi infect humans living in the Khanh Phu commune, Khanh Hoa Province, Vietnam. The latter species also infects wild macaque monkeys in this region. In order to understand the transmission dynamics of the three species, we attempted to detect gametocytes of the three species in the blood of infected individuals, and sporozoites in the salivary glands of mosquitoes from the same region. For the detection of gametocyte-specific mRNA, we targeted region 3 of pfg377, pvs25, pmg and pks25 as indicators of the presence of P. falciparum, P. vivax, P. malariae and P. knowlesi gametocytes, respectively. Gametocyte-specific mRNA was present in 37, 61, 0 and 47% of people infected with P. falciparum (n = 95), P. vivax (n = 69), P. malariae (n = 6) or P. knowlesi (n = 32), respectively. We found that 70% of mosquitoes that had P. knowlesi in their salivary glands also carried human malaria parasites, suggesting that mosquitoes are infected with P. knowlesi from human infections.

Original languageEnglish
Pages (from-to)527-535
Number of pages9
JournalParasitology
Volume144
Issue number4
DOIs
Publication statusPublished - 01-04-2017

Fingerprint

Plasmodium knowlesi
gametocytes
Vietnam
Culicidae
Coinfection
mixed infection
malaria
Plasmodium malariae
Malaria
Parasites
Plasmodium vivax
Plasmodium falciparum
parasites
Vivax Malaria
salivary glands
Salivary Glands
Sporozoites
Messenger RNA
Plasmodium
Falciparum Malaria

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Cite this

Maeno, Yoshimasa ; Culleton, R. ; Quang, N. T. ; Kawai, S. ; Marchand, R. P. ; Nakazawa, S. / Plasmodium knowlesi and human malaria parasites in Khan Phu, Vietnam : Gametocyte production in humans and frequent co-infection of mosquitoes. In: Parasitology. 2017 ; Vol. 144, No. 4. pp. 527-535.
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Plasmodium knowlesi and human malaria parasites in Khan Phu, Vietnam : Gametocyte production in humans and frequent co-infection of mosquitoes. / Maeno, Yoshimasa; Culleton, R.; Quang, N. T.; Kawai, S.; Marchand, R. P.; Nakazawa, S.

In: Parasitology, Vol. 144, No. 4, 01.04.2017, p. 527-535.

Research output: Contribution to journalArticle

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