Podocyte injuries exacerbate mesangial proliferative glomerulonephritis

Yoshio Morioka, Hiroko Koike, Yohei Ikezumi, Yumi Ito, Akihisa Oyanagi, Fumitake Gejyo, Fujio Shimizu, Hiroshi Kawachi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background. From the observations of morphology seen in early phases of the experimental models of the irreversible mesangial proliferative glomerulonephritis, we hypothesized that podocyte injury is one of the important factors in bringing upon irreversible glomerular alterations. To verify this hypothesis, we investigated whether podocyte injury induced by puromycin aminonucleoside (PAN) injection affects the mesangial alterations of anti-Thy 1.1 glomerulonephritis. Methods. Female Wistar rats were injected with 0.5 mg monoclonal antibody (mAb) 1-22-3 five days after the injection of 10 mg or 5 mg/100 g body weight (BW) of puromycin aminonucleoside (PAN), and sacrificed at 7 days or 8 weeks after the mAb 1-22-3 injection. Results. Consecutive injections of 10 mg/100 g BW of PAN and mAb 1-22-3 caused the irreversible mesangial alteration with persistent proteinuria (at week 8, proteinuria 100.3 ± 57.8 mg/24 h, matrix score 1.13 ± 0.52, collagen type I score 2.04 ± 0.53, mRNA for collagen type I 227 ± 79% to the group with a single injection of 1-22-3). Although single injection of 5 mg/100 g BW of PAN was not capable of inducing abnormal proteinuria, consecutive injections of 5 mg/100 g BW of PAN and mAb 1-22-3 also caused irreversible mesangial alteration and persistent proteinuria. Conclusions. Podocyte injury might be an important factor that exacerbates mesangial proliferation and mesangial matrix expansion. The irreversible mesangial alterations caused by consecutive injections of PAN and mAb 1-22-3 may be a novel model that could be used to analyze the mechanism of progressive mesangial alteration.

Original languageEnglish
Pages (from-to)2192-2204
Number of pages13
JournalKidney International
Volume60
Issue number6
DOIs
Publication statusPublished - 01-01-2001

Fingerprint

Podocytes
Puromycin Aminonucleoside
Glomerulonephritis
Injections
Wounds and Injuries
Proteinuria
Monoclonal Antibodies
Body Weight
Collagen Type I
Wistar Rats
Theoretical Models
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Morioka, Y., Koike, H., Ikezumi, Y., Ito, Y., Oyanagi, A., Gejyo, F., ... Kawachi, H. (2001). Podocyte injuries exacerbate mesangial proliferative glomerulonephritis. Kidney International, 60(6), 2192-2204. https://doi.org/10.1046/j.1523-1755.2001.00047.x
Morioka, Yoshio ; Koike, Hiroko ; Ikezumi, Yohei ; Ito, Yumi ; Oyanagi, Akihisa ; Gejyo, Fumitake ; Shimizu, Fujio ; Kawachi, Hiroshi. / Podocyte injuries exacerbate mesangial proliferative glomerulonephritis. In: Kidney International. 2001 ; Vol. 60, No. 6. pp. 2192-2204.
@article{af573869994048a9b90491a7fdd43358,
title = "Podocyte injuries exacerbate mesangial proliferative glomerulonephritis",
abstract = "Background. From the observations of morphology seen in early phases of the experimental models of the irreversible mesangial proliferative glomerulonephritis, we hypothesized that podocyte injury is one of the important factors in bringing upon irreversible glomerular alterations. To verify this hypothesis, we investigated whether podocyte injury induced by puromycin aminonucleoside (PAN) injection affects the mesangial alterations of anti-Thy 1.1 glomerulonephritis. Methods. Female Wistar rats were injected with 0.5 mg monoclonal antibody (mAb) 1-22-3 five days after the injection of 10 mg or 5 mg/100 g body weight (BW) of puromycin aminonucleoside (PAN), and sacrificed at 7 days or 8 weeks after the mAb 1-22-3 injection. Results. Consecutive injections of 10 mg/100 g BW of PAN and mAb 1-22-3 caused the irreversible mesangial alteration with persistent proteinuria (at week 8, proteinuria 100.3 ± 57.8 mg/24 h, matrix score 1.13 ± 0.52, collagen type I score 2.04 ± 0.53, mRNA for collagen type I 227 ± 79{\%} to the group with a single injection of 1-22-3). Although single injection of 5 mg/100 g BW of PAN was not capable of inducing abnormal proteinuria, consecutive injections of 5 mg/100 g BW of PAN and mAb 1-22-3 also caused irreversible mesangial alteration and persistent proteinuria. Conclusions. Podocyte injury might be an important factor that exacerbates mesangial proliferation and mesangial matrix expansion. The irreversible mesangial alterations caused by consecutive injections of PAN and mAb 1-22-3 may be a novel model that could be used to analyze the mechanism of progressive mesangial alteration.",
author = "Yoshio Morioka and Hiroko Koike and Yohei Ikezumi and Yumi Ito and Akihisa Oyanagi and Fumitake Gejyo and Fujio Shimizu and Hiroshi Kawachi",
year = "2001",
month = "1",
day = "1",
doi = "10.1046/j.1523-1755.2001.00047.x",
language = "English",
volume = "60",
pages = "2192--2204",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "6",

}

Morioka, Y, Koike, H, Ikezumi, Y, Ito, Y, Oyanagi, A, Gejyo, F, Shimizu, F & Kawachi, H 2001, 'Podocyte injuries exacerbate mesangial proliferative glomerulonephritis', Kidney International, vol. 60, no. 6, pp. 2192-2204. https://doi.org/10.1046/j.1523-1755.2001.00047.x

Podocyte injuries exacerbate mesangial proliferative glomerulonephritis. / Morioka, Yoshio; Koike, Hiroko; Ikezumi, Yohei; Ito, Yumi; Oyanagi, Akihisa; Gejyo, Fumitake; Shimizu, Fujio; Kawachi, Hiroshi.

In: Kidney International, Vol. 60, No. 6, 01.01.2001, p. 2192-2204.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Podocyte injuries exacerbate mesangial proliferative glomerulonephritis

AU - Morioka, Yoshio

AU - Koike, Hiroko

AU - Ikezumi, Yohei

AU - Ito, Yumi

AU - Oyanagi, Akihisa

AU - Gejyo, Fumitake

AU - Shimizu, Fujio

AU - Kawachi, Hiroshi

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Background. From the observations of morphology seen in early phases of the experimental models of the irreversible mesangial proliferative glomerulonephritis, we hypothesized that podocyte injury is one of the important factors in bringing upon irreversible glomerular alterations. To verify this hypothesis, we investigated whether podocyte injury induced by puromycin aminonucleoside (PAN) injection affects the mesangial alterations of anti-Thy 1.1 glomerulonephritis. Methods. Female Wistar rats were injected with 0.5 mg monoclonal antibody (mAb) 1-22-3 five days after the injection of 10 mg or 5 mg/100 g body weight (BW) of puromycin aminonucleoside (PAN), and sacrificed at 7 days or 8 weeks after the mAb 1-22-3 injection. Results. Consecutive injections of 10 mg/100 g BW of PAN and mAb 1-22-3 caused the irreversible mesangial alteration with persistent proteinuria (at week 8, proteinuria 100.3 ± 57.8 mg/24 h, matrix score 1.13 ± 0.52, collagen type I score 2.04 ± 0.53, mRNA for collagen type I 227 ± 79% to the group with a single injection of 1-22-3). Although single injection of 5 mg/100 g BW of PAN was not capable of inducing abnormal proteinuria, consecutive injections of 5 mg/100 g BW of PAN and mAb 1-22-3 also caused irreversible mesangial alteration and persistent proteinuria. Conclusions. Podocyte injury might be an important factor that exacerbates mesangial proliferation and mesangial matrix expansion. The irreversible mesangial alterations caused by consecutive injections of PAN and mAb 1-22-3 may be a novel model that could be used to analyze the mechanism of progressive mesangial alteration.

AB - Background. From the observations of morphology seen in early phases of the experimental models of the irreversible mesangial proliferative glomerulonephritis, we hypothesized that podocyte injury is one of the important factors in bringing upon irreversible glomerular alterations. To verify this hypothesis, we investigated whether podocyte injury induced by puromycin aminonucleoside (PAN) injection affects the mesangial alterations of anti-Thy 1.1 glomerulonephritis. Methods. Female Wistar rats were injected with 0.5 mg monoclonal antibody (mAb) 1-22-3 five days after the injection of 10 mg or 5 mg/100 g body weight (BW) of puromycin aminonucleoside (PAN), and sacrificed at 7 days or 8 weeks after the mAb 1-22-3 injection. Results. Consecutive injections of 10 mg/100 g BW of PAN and mAb 1-22-3 caused the irreversible mesangial alteration with persistent proteinuria (at week 8, proteinuria 100.3 ± 57.8 mg/24 h, matrix score 1.13 ± 0.52, collagen type I score 2.04 ± 0.53, mRNA for collagen type I 227 ± 79% to the group with a single injection of 1-22-3). Although single injection of 5 mg/100 g BW of PAN was not capable of inducing abnormal proteinuria, consecutive injections of 5 mg/100 g BW of PAN and mAb 1-22-3 also caused irreversible mesangial alteration and persistent proteinuria. Conclusions. Podocyte injury might be an important factor that exacerbates mesangial proliferation and mesangial matrix expansion. The irreversible mesangial alterations caused by consecutive injections of PAN and mAb 1-22-3 may be a novel model that could be used to analyze the mechanism of progressive mesangial alteration.

UR - http://www.scopus.com/inward/record.url?scp=0035185857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035185857&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1755.2001.00047.x

DO - 10.1046/j.1523-1755.2001.00047.x

M3 - Article

VL - 60

SP - 2192

EP - 2204

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 6

ER -