Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP

Yusuke Kimura; Masashi Ikeuchi; Yoshinori Inoue; Koji Ikuta

doi:10.1109/MEMSYS.2019.8870711

Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP

Yusuke Kimura
, Masashi Ikeuchi
, Yoshinori Inoue
, Koji Ikuta

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

Here, we developed a portable microdevice for the simple and early detection of viral infections via the loop-mediated isothermal amplification (LAMP) method, as a point-of-care testing (POCT) method. This microdevice can simultaneously check 16 species of viral infection by observing fluorescence intensity, either by the naked eye or using a smartphone photo sensor. It requires only 1% of conventional sample amounts, and can run using a small battery. We also developed a simple system for simultaneously applying samples to 16 wells. Basic performance was verified using a dengue virus detection experiment, and the specific reaction was successfully confirmed following application of dengue virus RNA samples.

Original language	English
Title of host publication	2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	83-86
Number of pages	4
ISBN (Electronic)	9781728116105
DOIs	https://doi.org/10.1109/MEMSYS.2019.8870711
Publication status	Published - 01-2019
Externally published	Yes
Event	32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 - Seoul, Korea, Republic of Duration: 27-01-2019 → 31-01-2019

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2019-January
ISSN (Print)	1084-6999

Conference

Conference	32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019
Country/Territory	Korea, Republic of
City	Seoul
Period	27-01-19 → 31-01-19

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2019.8870711

Cite this

Kimura, Y., Ikeuchi, M., Inoue, Y., & Ikuta, K. (2019). Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019 (pp. 83-86). Article 8870711 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2019.8870711

Kimura, Yusuke ; Ikeuchi, Masashi ; Inoue, Yoshinori et al. / Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP. 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 83-86 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{01a59eeae1f24b3cb44ae4c262a60bdb,

title = "Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP",

abstract = "Here, we developed a portable microdevice for the simple and early detection of viral infections via the loop-mediated isothermal amplification (LAMP) method, as a point-of-care testing (POCT) method. This microdevice can simultaneously check 16 species of viral infection by observing fluorescence intensity, either by the naked eye or using a smartphone photo sensor. It requires only 1\% of conventional sample amounts, and can run using a small battery. We also developed a simple system for simultaneously applying samples to 16 wells. Basic performance was verified using a dengue virus detection experiment, and the specific reaction was successfully confirmed following application of dengue virus RNA samples.",

author = "Yusuke Kimura and Masashi Ikeuchi and Yoshinori Inoue and Koji Ikuta",

note = "Publisher Copyright: {\textcopyright} 2019 IEEE.; 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 ; Conference date: 27-01-2019 Through 31-01-2019",

year = "2019",

month = jan,

doi = "10.1109/MEMSYS.2019.8870711",

language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "83--86",

booktitle = "2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019",

address = "United States",

}

Kimura, Y, Ikeuchi, M, Inoue, Y & Ikuta, K 2019, Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP. in 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019., 8870711, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2019-January, Institute of Electrical and Electronics Engineers Inc., pp. 83-86, 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019, Seoul, Korea, Republic of, 27-01-19. https://doi.org/10.1109/MEMSYS.2019.8870711

Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP. / Kimura, Yusuke; Ikeuchi, Masashi; Inoue, Yoshinori et al.
2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc., 2019. p. 83-86 8870711 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP

AU - Kimura, Yusuke

AU - Ikeuchi, Masashi

AU - Inoue, Yoshinori

AU - Ikuta, Koji

PY - 2019/1

Y1 - 2019/1

N2 - Here, we developed a portable microdevice for the simple and early detection of viral infections via the loop-mediated isothermal amplification (LAMP) method, as a point-of-care testing (POCT) method. This microdevice can simultaneously check 16 species of viral infection by observing fluorescence intensity, either by the naked eye or using a smartphone photo sensor. It requires only 1% of conventional sample amounts, and can run using a small battery. We also developed a simple system for simultaneously applying samples to 16 wells. Basic performance was verified using a dengue virus detection experiment, and the specific reaction was successfully confirmed following application of dengue virus RNA samples.

AB - Here, we developed a portable microdevice for the simple and early detection of viral infections via the loop-mediated isothermal amplification (LAMP) method, as a point-of-care testing (POCT) method. This microdevice can simultaneously check 16 species of viral infection by observing fluorescence intensity, either by the naked eye or using a smartphone photo sensor. It requires only 1% of conventional sample amounts, and can run using a small battery. We also developed a simple system for simultaneously applying samples to 16 wells. Basic performance was verified using a dengue virus detection experiment, and the specific reaction was successfully confirmed following application of dengue virus RNA samples.

UR - https://www.scopus.com/pages/publications/85074366249

UR - https://www.scopus.com/pages/publications/85074366249#tab=citedBy

U2 - 10.1109/MEMSYS.2019.8870711

DO - 10.1109/MEMSYS.2019.8870711

M3 - Conference contribution

AN - SCOPUS:85074366249

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 83

EP - 86

BT - 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019

Y2 - 27 January 2019 through 31 January 2019

ER -

Kimura Y, Ikeuchi M, Inoue Y, Ikuta K. Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc. 2019. p. 83-86. 8870711. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2019.8870711

Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP

Abstract

Publication series

Conference

UN SDGs

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this