Polymorphism rs7521584 in miR-429 is associated with the severity of atrophic gastritis in patients with Helicobacter pylori infection

Toshimi Otsuka, Tomomitsu Tahara, Masakatsu Nakamura, Wu Jing, Masafumi Ota, Tomoe Nomura, Ranji Hayashi, Takeo Shimasaki, Tomoyuki Shibata, Tomiyasu Arisawa

Research output: Contribution to journalArticle

Abstract

The aim of the present study was to investigate an association of genetic polymorphism (rs7521584) located in miR-200a-200b-429 cluster, which has tumor suppressor and pro-inflammatory function, with the development of gastric mucosal atrophy and metaplasia as a pre-malignant condition. Gastric mucosa samples were obtained from the antrum of 393 patients with no malignancies. The rs7521584 genotype was determined using the polymerase chain reaction-single-strand conformation polymorphism analysis method. The degree of gastritis was assessed histologically in all subjects and serum levels of pepsinogen (PG) I/II were quantified in 123 out of 393 patients. Patients with an atrophy score =1 and metaplasia score =1 were classified into the atrophic gastritis group (AG group). The rs7521584 TT genotype was significantly associated with the development of atrophic gastritis [odds ratio (OR), 2.41; 95% confidence interval (CI), 1.10-5.25; P=0.027), particularly in patients with H. pyloriinfection (OR, 3.31; 95% CI, 1.35-8.12; P=0.0089). In addition, in patients younger than 60 years of age, this genotype was associated with atrophic gastritis (OR, 3.15; 95% CI 1.03-9.61; P=0.044)]. In patients with H. pyloriinfection, the metaplasia score was significantly higher in the TT homozygote compared with the GG+GT genotype. In the rs7521584 TT homozygote, serum PG I/II ratio was significantly reduced with increasing age (P=0.0084). No significant trend was identified between the GG+GT genotype and age. The results of the current study indicated that the rs7521584 minor allele homozygote was associated with the development of chronic gastritis under the influence of H. pylori-induced inflammation, particularly with the severity of metaplastic alterations.

Original languageEnglish
Pages (from-to)2381-2386
Number of pages6
JournalMolecular Medicine Reports
Volume18
Issue number2
DOIs
Publication statusPublished - 01-08-2018

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Pepsinogen A
Atrophic Gastritis
Helicobacter Infections
Polymorphism
Helicobacter pylori
Genotype
Polymerase chain reaction
Metaplasia
Homozygote
Pepsinogen C
Conformations
Tumors
Odds Ratio
Association reactions
Gastritis
Confidence Intervals
Atrophy
Genetic Polymorphisms
Gastric Mucosa
Serum

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

Cite this

Otsuka, Toshimi ; Tahara, Tomomitsu ; Nakamura, Masakatsu ; Jing, Wu ; Ota, Masafumi ; Nomura, Tomoe ; Hayashi, Ranji ; Shimasaki, Takeo ; Shibata, Tomoyuki ; Arisawa, Tomiyasu. / Polymorphism rs7521584 in miR-429 is associated with the severity of atrophic gastritis in patients with Helicobacter pylori infection. In: Molecular Medicine Reports. 2018 ; Vol. 18, No. 2. pp. 2381-2386.
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title = "Polymorphism rs7521584 in miR-429 is associated with the severity of atrophic gastritis in patients with Helicobacter pylori infection",
abstract = "The aim of the present study was to investigate an association of genetic polymorphism (rs7521584) located in miR-200a-200b-429 cluster, which has tumor suppressor and pro-inflammatory function, with the development of gastric mucosal atrophy and metaplasia as a pre-malignant condition. Gastric mucosa samples were obtained from the antrum of 393 patients with no malignancies. The rs7521584 genotype was determined using the polymerase chain reaction-single-strand conformation polymorphism analysis method. The degree of gastritis was assessed histologically in all subjects and serum levels of pepsinogen (PG) I/II were quantified in 123 out of 393 patients. Patients with an atrophy score =1 and metaplasia score =1 were classified into the atrophic gastritis group (AG group). The rs7521584 TT genotype was significantly associated with the development of atrophic gastritis [odds ratio (OR), 2.41; 95{\%} confidence interval (CI), 1.10-5.25; P=0.027), particularly in patients with H. pyloriinfection (OR, 3.31; 95{\%} CI, 1.35-8.12; P=0.0089). In addition, in patients younger than 60 years of age, this genotype was associated with atrophic gastritis (OR, 3.15; 95{\%} CI 1.03-9.61; P=0.044)]. In patients with H. pyloriinfection, the metaplasia score was significantly higher in the TT homozygote compared with the GG+GT genotype. In the rs7521584 TT homozygote, serum PG I/II ratio was significantly reduced with increasing age (P=0.0084). No significant trend was identified between the GG+GT genotype and age. The results of the current study indicated that the rs7521584 minor allele homozygote was associated with the development of chronic gastritis under the influence of H. pylori-induced inflammation, particularly with the severity of metaplastic alterations.",
author = "Toshimi Otsuka and Tomomitsu Tahara and Masakatsu Nakamura and Wu Jing and Masafumi Ota and Tomoe Nomura and Ranji Hayashi and Takeo Shimasaki and Tomoyuki Shibata and Tomiyasu Arisawa",
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Otsuka, T, Tahara, T, Nakamura, M, Jing, W, Ota, M, Nomura, T, Hayashi, R, Shimasaki, T, Shibata, T & Arisawa, T 2018, 'Polymorphism rs7521584 in miR-429 is associated with the severity of atrophic gastritis in patients with Helicobacter pylori infection', Molecular Medicine Reports, vol. 18, no. 2, pp. 2381-2386. https://doi.org/10.3892/mmr.2018.9200

Polymorphism rs7521584 in miR-429 is associated with the severity of atrophic gastritis in patients with Helicobacter pylori infection. / Otsuka, Toshimi; Tahara, Tomomitsu; Nakamura, Masakatsu; Jing, Wu; Ota, Masafumi; Nomura, Tomoe; Hayashi, Ranji; Shimasaki, Takeo; Shibata, Tomoyuki; Arisawa, Tomiyasu.

In: Molecular Medicine Reports, Vol. 18, No. 2, 01.08.2018, p. 2381-2386.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Polymorphism rs7521584 in miR-429 is associated with the severity of atrophic gastritis in patients with Helicobacter pylori infection

AU - Otsuka, Toshimi

AU - Tahara, Tomomitsu

AU - Nakamura, Masakatsu

AU - Jing, Wu

AU - Ota, Masafumi

AU - Nomura, Tomoe

AU - Hayashi, Ranji

AU - Shimasaki, Takeo

AU - Shibata, Tomoyuki

AU - Arisawa, Tomiyasu

PY - 2018/8/1

Y1 - 2018/8/1

N2 - The aim of the present study was to investigate an association of genetic polymorphism (rs7521584) located in miR-200a-200b-429 cluster, which has tumor suppressor and pro-inflammatory function, with the development of gastric mucosal atrophy and metaplasia as a pre-malignant condition. Gastric mucosa samples were obtained from the antrum of 393 patients with no malignancies. The rs7521584 genotype was determined using the polymerase chain reaction-single-strand conformation polymorphism analysis method. The degree of gastritis was assessed histologically in all subjects and serum levels of pepsinogen (PG) I/II were quantified in 123 out of 393 patients. Patients with an atrophy score =1 and metaplasia score =1 were classified into the atrophic gastritis group (AG group). The rs7521584 TT genotype was significantly associated with the development of atrophic gastritis [odds ratio (OR), 2.41; 95% confidence interval (CI), 1.10-5.25; P=0.027), particularly in patients with H. pyloriinfection (OR, 3.31; 95% CI, 1.35-8.12; P=0.0089). In addition, in patients younger than 60 years of age, this genotype was associated with atrophic gastritis (OR, 3.15; 95% CI 1.03-9.61; P=0.044)]. In patients with H. pyloriinfection, the metaplasia score was significantly higher in the TT homozygote compared with the GG+GT genotype. In the rs7521584 TT homozygote, serum PG I/II ratio was significantly reduced with increasing age (P=0.0084). No significant trend was identified between the GG+GT genotype and age. The results of the current study indicated that the rs7521584 minor allele homozygote was associated with the development of chronic gastritis under the influence of H. pylori-induced inflammation, particularly with the severity of metaplastic alterations.

AB - The aim of the present study was to investigate an association of genetic polymorphism (rs7521584) located in miR-200a-200b-429 cluster, which has tumor suppressor and pro-inflammatory function, with the development of gastric mucosal atrophy and metaplasia as a pre-malignant condition. Gastric mucosa samples were obtained from the antrum of 393 patients with no malignancies. The rs7521584 genotype was determined using the polymerase chain reaction-single-strand conformation polymorphism analysis method. The degree of gastritis was assessed histologically in all subjects and serum levels of pepsinogen (PG) I/II were quantified in 123 out of 393 patients. Patients with an atrophy score =1 and metaplasia score =1 were classified into the atrophic gastritis group (AG group). The rs7521584 TT genotype was significantly associated with the development of atrophic gastritis [odds ratio (OR), 2.41; 95% confidence interval (CI), 1.10-5.25; P=0.027), particularly in patients with H. pyloriinfection (OR, 3.31; 95% CI, 1.35-8.12; P=0.0089). In addition, in patients younger than 60 years of age, this genotype was associated with atrophic gastritis (OR, 3.15; 95% CI 1.03-9.61; P=0.044)]. In patients with H. pyloriinfection, the metaplasia score was significantly higher in the TT homozygote compared with the GG+GT genotype. In the rs7521584 TT homozygote, serum PG I/II ratio was significantly reduced with increasing age (P=0.0084). No significant trend was identified between the GG+GT genotype and age. The results of the current study indicated that the rs7521584 minor allele homozygote was associated with the development of chronic gastritis under the influence of H. pylori-induced inflammation, particularly with the severity of metaplastic alterations.

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