Polymorphisms in the lymphotoxin alpha gene and the risk of ischemic stroke in the Japanese population

The Fukuoka Stroke Registry and the Hisayama Study

Noriko Hagiwara, Takanari Kitazono, Masahiro Kamouchi, Junya Kuroda, Tetsuro Ago, Jun Hata, Toshiharu Ninomiya, Hiroaki Ooboshi, Yasuhiro Kumai, Sohei Yoshimura, Kinya Tamaki, Kenichiro Fujii, Tetsuhiko Nagao, Yasushi Okada, Kazunori Toyoda, Hiroshi Nakane, Hiroshi Sugimori, Yoshichika Yamashita, Yoshiyuki Wakugawa, Michiaki Kubo & 4 others Yumihiro Tanizaki, Yutaka Kiyohara, Setsuro Ibayashi, Mitsuo Iida

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background and Purpose: Lymphotoxin α (LTA), one of the tumor necrosis factor family proteins, is an important proinflammatory cytokine and appears to play a putative role in the inflammatory process of atherosclerosis. Recent genetic studies have suggested that variations in the gene encoding LTA, which affect its expression and biological function, may contribute to the development of vascular diseases. We conducted a case-control study to clarify the association of LTA gene polymorphisms with ischemic stroke in a large Japanese population. Methods: Genotyping for LTA A252G and C804A polymorphisms was achieved by a rapid-cycle polymerase chain reaction and melting curve analysis using fluorescent probes in 1,044 incident cases of ischemic stroke recruited from the Fukuoka Stroke Registry and 1,044 age- and sex-matched control subjects recruited from the Hisayama Study. Results: The overall distribution of allele and genotype for each polymorphism was similar between stroke patients and control subjects. The allele frequencies of 252G and 804A were slightly lower in stroke patients than in control subjects; however, conditional logistic regression analysis adjusted for potential risk factors found no association between the risk of ischemic stroke and either polymorphism. In terms of stroke subtype, we also found no association of these polymorphisms with any subtypes of ischemic stroke. Conclusions: Neither the A252G nor C804A polymorphism of the LTA gene was associated with stroke overall and any subtypes of ischemic stroke in the Japanese population.

Original languageEnglish
Pages (from-to)417-422
Number of pages6
JournalCerebrovascular Diseases
Volume25
Issue number5
DOIs
Publication statusPublished - 01-05-2008
Externally publishedYes

Fingerprint

Lymphotoxin-alpha
Registries
Stroke
Population
Genes
Vascular Diseases
Fluorescent Dyes
Gene Frequency
Freezing
Case-Control Studies
Atherosclerosis
Tumor Necrosis Factor-alpha
Logistic Models
Alleles
Genotype
Regression Analysis
Cytokines

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Hagiwara, Noriko ; Kitazono, Takanari ; Kamouchi, Masahiro ; Kuroda, Junya ; Ago, Tetsuro ; Hata, Jun ; Ninomiya, Toshiharu ; Ooboshi, Hiroaki ; Kumai, Yasuhiro ; Yoshimura, Sohei ; Tamaki, Kinya ; Fujii, Kenichiro ; Nagao, Tetsuhiko ; Okada, Yasushi ; Toyoda, Kazunori ; Nakane, Hiroshi ; Sugimori, Hiroshi ; Yamashita, Yoshichika ; Wakugawa, Yoshiyuki ; Kubo, Michiaki ; Tanizaki, Yumihiro ; Kiyohara, Yutaka ; Ibayashi, Setsuro ; Iida, Mitsuo. / Polymorphisms in the lymphotoxin alpha gene and the risk of ischemic stroke in the Japanese population : The Fukuoka Stroke Registry and the Hisayama Study. In: Cerebrovascular Diseases. 2008 ; Vol. 25, No. 5. pp. 417-422.
@article{1e82f993ac174f39b15813fb1cd199d7,
title = "Polymorphisms in the lymphotoxin alpha gene and the risk of ischemic stroke in the Japanese population: The Fukuoka Stroke Registry and the Hisayama Study",
abstract = "Background and Purpose: Lymphotoxin α (LTA), one of the tumor necrosis factor family proteins, is an important proinflammatory cytokine and appears to play a putative role in the inflammatory process of atherosclerosis. Recent genetic studies have suggested that variations in the gene encoding LTA, which affect its expression and biological function, may contribute to the development of vascular diseases. We conducted a case-control study to clarify the association of LTA gene polymorphisms with ischemic stroke in a large Japanese population. Methods: Genotyping for LTA A252G and C804A polymorphisms was achieved by a rapid-cycle polymerase chain reaction and melting curve analysis using fluorescent probes in 1,044 incident cases of ischemic stroke recruited from the Fukuoka Stroke Registry and 1,044 age- and sex-matched control subjects recruited from the Hisayama Study. Results: The overall distribution of allele and genotype for each polymorphism was similar between stroke patients and control subjects. The allele frequencies of 252G and 804A were slightly lower in stroke patients than in control subjects; however, conditional logistic regression analysis adjusted for potential risk factors found no association between the risk of ischemic stroke and either polymorphism. In terms of stroke subtype, we also found no association of these polymorphisms with any subtypes of ischemic stroke. Conclusions: Neither the A252G nor C804A polymorphism of the LTA gene was associated with stroke overall and any subtypes of ischemic stroke in the Japanese population.",
author = "Noriko Hagiwara and Takanari Kitazono and Masahiro Kamouchi and Junya Kuroda and Tetsuro Ago and Jun Hata and Toshiharu Ninomiya and Hiroaki Ooboshi and Yasuhiro Kumai and Sohei Yoshimura and Kinya Tamaki and Kenichiro Fujii and Tetsuhiko Nagao and Yasushi Okada and Kazunori Toyoda and Hiroshi Nakane and Hiroshi Sugimori and Yoshichika Yamashita and Yoshiyuki Wakugawa and Michiaki Kubo and Yumihiro Tanizaki and Yutaka Kiyohara and Setsuro Ibayashi and Mitsuo Iida",
year = "2008",
month = "5",
day = "1",
doi = "10.1159/000121342",
language = "English",
volume = "25",
pages = "417--422",
journal = "Cerebrovascular Diseases",
issn = "1015-9770",
publisher = "S. Karger AG",
number = "5",

}

Hagiwara, N, Kitazono, T, Kamouchi, M, Kuroda, J, Ago, T, Hata, J, Ninomiya, T, Ooboshi, H, Kumai, Y, Yoshimura, S, Tamaki, K, Fujii, K, Nagao, T, Okada, Y, Toyoda, K, Nakane, H, Sugimori, H, Yamashita, Y, Wakugawa, Y, Kubo, M, Tanizaki, Y, Kiyohara, Y, Ibayashi, S & Iida, M 2008, 'Polymorphisms in the lymphotoxin alpha gene and the risk of ischemic stroke in the Japanese population: The Fukuoka Stroke Registry and the Hisayama Study', Cerebrovascular Diseases, vol. 25, no. 5, pp. 417-422. https://doi.org/10.1159/000121342

Polymorphisms in the lymphotoxin alpha gene and the risk of ischemic stroke in the Japanese population : The Fukuoka Stroke Registry and the Hisayama Study. / Hagiwara, Noriko; Kitazono, Takanari; Kamouchi, Masahiro; Kuroda, Junya; Ago, Tetsuro; Hata, Jun; Ninomiya, Toshiharu; Ooboshi, Hiroaki; Kumai, Yasuhiro; Yoshimura, Sohei; Tamaki, Kinya; Fujii, Kenichiro; Nagao, Tetsuhiko; Okada, Yasushi; Toyoda, Kazunori; Nakane, Hiroshi; Sugimori, Hiroshi; Yamashita, Yoshichika; Wakugawa, Yoshiyuki; Kubo, Michiaki; Tanizaki, Yumihiro; Kiyohara, Yutaka; Ibayashi, Setsuro; Iida, Mitsuo.

In: Cerebrovascular Diseases, Vol. 25, No. 5, 01.05.2008, p. 417-422.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Polymorphisms in the lymphotoxin alpha gene and the risk of ischemic stroke in the Japanese population

T2 - The Fukuoka Stroke Registry and the Hisayama Study

AU - Hagiwara, Noriko

AU - Kitazono, Takanari

AU - Kamouchi, Masahiro

AU - Kuroda, Junya

AU - Ago, Tetsuro

AU - Hata, Jun

AU - Ninomiya, Toshiharu

AU - Ooboshi, Hiroaki

AU - Kumai, Yasuhiro

AU - Yoshimura, Sohei

AU - Tamaki, Kinya

AU - Fujii, Kenichiro

AU - Nagao, Tetsuhiko

AU - Okada, Yasushi

AU - Toyoda, Kazunori

AU - Nakane, Hiroshi

AU - Sugimori, Hiroshi

AU - Yamashita, Yoshichika

AU - Wakugawa, Yoshiyuki

AU - Kubo, Michiaki

AU - Tanizaki, Yumihiro

AU - Kiyohara, Yutaka

AU - Ibayashi, Setsuro

AU - Iida, Mitsuo

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Background and Purpose: Lymphotoxin α (LTA), one of the tumor necrosis factor family proteins, is an important proinflammatory cytokine and appears to play a putative role in the inflammatory process of atherosclerosis. Recent genetic studies have suggested that variations in the gene encoding LTA, which affect its expression and biological function, may contribute to the development of vascular diseases. We conducted a case-control study to clarify the association of LTA gene polymorphisms with ischemic stroke in a large Japanese population. Methods: Genotyping for LTA A252G and C804A polymorphisms was achieved by a rapid-cycle polymerase chain reaction and melting curve analysis using fluorescent probes in 1,044 incident cases of ischemic stroke recruited from the Fukuoka Stroke Registry and 1,044 age- and sex-matched control subjects recruited from the Hisayama Study. Results: The overall distribution of allele and genotype for each polymorphism was similar between stroke patients and control subjects. The allele frequencies of 252G and 804A were slightly lower in stroke patients than in control subjects; however, conditional logistic regression analysis adjusted for potential risk factors found no association between the risk of ischemic stroke and either polymorphism. In terms of stroke subtype, we also found no association of these polymorphisms with any subtypes of ischemic stroke. Conclusions: Neither the A252G nor C804A polymorphism of the LTA gene was associated with stroke overall and any subtypes of ischemic stroke in the Japanese population.

AB - Background and Purpose: Lymphotoxin α (LTA), one of the tumor necrosis factor family proteins, is an important proinflammatory cytokine and appears to play a putative role in the inflammatory process of atherosclerosis. Recent genetic studies have suggested that variations in the gene encoding LTA, which affect its expression and biological function, may contribute to the development of vascular diseases. We conducted a case-control study to clarify the association of LTA gene polymorphisms with ischemic stroke in a large Japanese population. Methods: Genotyping for LTA A252G and C804A polymorphisms was achieved by a rapid-cycle polymerase chain reaction and melting curve analysis using fluorescent probes in 1,044 incident cases of ischemic stroke recruited from the Fukuoka Stroke Registry and 1,044 age- and sex-matched control subjects recruited from the Hisayama Study. Results: The overall distribution of allele and genotype for each polymorphism was similar between stroke patients and control subjects. The allele frequencies of 252G and 804A were slightly lower in stroke patients than in control subjects; however, conditional logistic regression analysis adjusted for potential risk factors found no association between the risk of ischemic stroke and either polymorphism. In terms of stroke subtype, we also found no association of these polymorphisms with any subtypes of ischemic stroke. Conclusions: Neither the A252G nor C804A polymorphism of the LTA gene was associated with stroke overall and any subtypes of ischemic stroke in the Japanese population.

UR - http://www.scopus.com/inward/record.url?scp=44449118037&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44449118037&partnerID=8YFLogxK

U2 - 10.1159/000121342

DO - 10.1159/000121342

M3 - Article

VL - 25

SP - 417

EP - 422

JO - Cerebrovascular Diseases

JF - Cerebrovascular Diseases

SN - 1015-9770

IS - 5

ER -