Porphyromonas bronchialis sp. nov. Isolated from intraoperative bronchial fluids of a patient with non-small cell lung cancer

Takuichi Sato, Junko Tomida, Takashi Naka, Nagatoshi Fujiwara, Ayako Hasegawa, Yasushi Hoshikawa, Junko Matsuyama, Naoko Ishida, Takashi Kondo, Kaori Tanaka, Nobuhiro Takahashi, Yoshiaki Kawamura

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Abstract

Porphyromonas strains, including Porphyromonas-like strains, have been isolated from oral and various other systemic infections. The characterization of such strains is a crucial issue, because such information contributes to both the taxonomy of anaerobic bacteria and the clinical aspects of infectious diseases. We previously isolated four Porphyromonas-like strains from intraoperative bronchial fluids of a patient with non-small cell lung cancer. This study aimed to characterize the genetic, biochemical and chemotaxonomic aspects of these isolates. Each strain only grew under anaerobic conditions and their colony morphology was convex, 0.1-1.0 mm in diameter, light gray, and slightly glistening colony, with no black or brown pigmentation on blood agar plates after five-day incubation. The pigmentation was helpful to differentiate the isolates from other Porphyromonas, as most of Porphyromonas species show the pigmentation. In the 16S rRNA gene phylogenetic analysis (98% sequence identity of isolates indicates the same species), the four isolates were closely related to one another (99.7-100.0%), but not related to Porphyromonas (P.) catoniae, the closest species (96.9%). In addition, the DNA-DNA hybridization data revealed less than 16% similarity values between a representative isolate and the P. catoniae, indicating that the strains were genetically independent. Biochemically, the isolates could be differentiated from closely related species, i.e., P. catoniae, P. gingivalis, P. gulae, and P. pogonae, with trypsin activity (negative only in the isolates) and leucine arylamidase activity (positive only in the isolates). We therefore propose a new species to include these isolates: Porphyromonas bronchialis sp. nov.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalTohoku Journal of Experimental Medicine
Volume237
Issue number1
DOIs
Publication statusPublished - 01-01-2015

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Porphyromonas
Non-Small Cell Lung Carcinoma
Cells
Fluids
Pigmentation
DNA
Taxonomies
Anaerobic Bacteria
Leucine
Trypsin
Agar
rRNA Genes
Bacteria
Blood
Genes
Communicable Diseases
Sequence Analysis
Molecular Biology
Light

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sato, Takuichi ; Tomida, Junko ; Naka, Takashi ; Fujiwara, Nagatoshi ; Hasegawa, Ayako ; Hoshikawa, Yasushi ; Matsuyama, Junko ; Ishida, Naoko ; Kondo, Takashi ; Tanaka, Kaori ; Takahashi, Nobuhiro ; Kawamura, Yoshiaki. / Porphyromonas bronchialis sp. nov. Isolated from intraoperative bronchial fluids of a patient with non-small cell lung cancer. In: Tohoku Journal of Experimental Medicine. 2015 ; Vol. 237, No. 1. pp. 31-37.
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Sato, T, Tomida, J, Naka, T, Fujiwara, N, Hasegawa, A, Hoshikawa, Y, Matsuyama, J, Ishida, N, Kondo, T, Tanaka, K, Takahashi, N & Kawamura, Y 2015, 'Porphyromonas bronchialis sp. nov. Isolated from intraoperative bronchial fluids of a patient with non-small cell lung cancer', Tohoku Journal of Experimental Medicine, vol. 237, no. 1, pp. 31-37. https://doi.org/10.1620/tjem.237.31

Porphyromonas bronchialis sp. nov. Isolated from intraoperative bronchial fluids of a patient with non-small cell lung cancer. / Sato, Takuichi; Tomida, Junko; Naka, Takashi; Fujiwara, Nagatoshi; Hasegawa, Ayako; Hoshikawa, Yasushi; Matsuyama, Junko; Ishida, Naoko; Kondo, Takashi; Tanaka, Kaori; Takahashi, Nobuhiro; Kawamura, Yoshiaki.

In: Tohoku Journal of Experimental Medicine, Vol. 237, No. 1, 01.01.2015, p. 31-37.

Research output: Contribution to journalArticle

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T1 - Porphyromonas bronchialis sp. nov. Isolated from intraoperative bronchial fluids of a patient with non-small cell lung cancer

AU - Sato, Takuichi

AU - Tomida, Junko

AU - Naka, Takashi

AU - Fujiwara, Nagatoshi

AU - Hasegawa, Ayako

AU - Hoshikawa, Yasushi

AU - Matsuyama, Junko

AU - Ishida, Naoko

AU - Kondo, Takashi

AU - Tanaka, Kaori

AU - Takahashi, Nobuhiro

AU - Kawamura, Yoshiaki

PY - 2015/1/1

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